The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.
Alef K. and Nannipieri P., (Eds), 1995. Protease activity. In: Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London, UK.
Alkorta I., Aizpurua A., Riga P., Albizu I., Amezaga I., andGarbisu C., 2003. Soil enzyme activities as biological indicators of soil health. Rev. Environ. Health, 18, 65-73.
Beyer A. and Biziuk M., 2007. Methods of determination of residues of pesticides and polychlorinated biphenyls in food samples - a review. Ecol. Chem. Eng., 14, 35-58.
Caldwell B.A., 2005. Enzyme activities as a component of soil biodiversity: A review. Pedobiologia, 49, 637-644.
Chowdhury A., Pradhan S., Saha M., and Sanyal N., 2008. Impact of pesticides on soil microbiological parameters and possible bioremediation strategies. Indian J. Microbiol., 48, 114-127.
Cycoń M. and Piotrowska-Seget Z., 2007. Effect of selected pesticides on soil microflora involved in organic matter and nitrogen transformations: pot experiment. Polish J. Ecol., 55, 207-220.
Das A.C., Debnath A., and Mukherjee D., 2003. Effect of the herbicides oxadiazon and oxyfluorfen on phosphates solubilizing microorganisms and their persistence in rice fields. Chemosphere, 53, 217-221.
Das A.C. and Mukherjee D., 2000. Soil application of insecticides influences microorganisms and plant nutrients. Appl. Soil Ecol., 14, 55-62.
Janvier C., Villeneuve I. F., Alabouvette C., Edel-Hermenn V.,Mateille T., and Steinberg C., 2007. Soil health through soil disease suppression: Which strategy from descriptors to indicators? Soil Biol. Biochem., 39, 1-23.
Kaszubiak H. and Durska G., 2000. Effect of Oxafun T seed dressing on bacteria in rhizosphere and non-rhizosphere soil. Polish J. Environ. Stud., 9, 397-401.
Kucharski J., Baćmaga M., and Wyszkowska J., 2009. Enzymatic activity of soil polluted with herbicide Harpun 500 SC (in Polish). Adv. Agric Sci., 540, 225-236.
Ladd J.N. and Butler J.H.A., 1972. Short-terms assays of soil proteolytic enzyme activities using proteins and dipetide derivaties as substrates. Soil Biol. Biochem., 4, 19-30.
Martin J.P., 1950. Use of acid rose bengal and streptomycin in the plate method for estimating soil fungi. Soil. Sci., 69, 215-232.
Omar S.A. and Abdel-Sater M.A., 2001. Microbial populations and enzyme activities in soil treated with pesticides. Water, Air, Soil Poll., 127, 49-63.
Popova L.,Ananieva E., Hristova V.,Georgieva K.,AlexievaV.,and Stoinova Zh., 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian J. Plant Physiol., 133-152.
Rasool L.N. and Reshi Z., 2010. Effect of the fungicide Mancozeb at different application rates on enzyme activities in a silt loam soil of Kashmir Himalaya, Indian Trop. Ecol., 51, 199-205.
Sebiomo A., Ogundero V.W., and Bankole S.A., 2011. Utilisation and biodegradation of atrazine and primextra. J. Microbiol. Antimicrob., 3, 64-76.
Sławiński C., Cymerman J., Witkowska-Walczak B., andLamorski K., 2012. Impact of diverse tillage on soil moisture dynamics. Int. Agrophys., 26, 301-309.
Szatanik-Kloc A., 2012. Effect of pH and zinc stress on micropore system of rye roots. Int. Agrophys., 26, 311-316.
Tabatabai M.A. and Bremner J.M., 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem., 1, 301-307.
Thalmann A., 1968. Zur Methodik der Bestimmung der dehydrogenaseactivität im boden mittels triphenyltetrazoliumchlorid (TTC). Landwirtsh. Forsch., 21, 249-258.
Trolldenier G., 1995. Bacterial biomass. In: Methods in Soil Biology (Eds F. Schinner, R. Öhlinger, E. Kandeler, R. Margesin). Springer Press, Berlin, Germany.
Wang Q.Y., Zhou D.M., and Cang L., 2009. Microbial and enzyme properties of apple orchard soil as affected by long-term application of copper fungicide. Soil Biol. Biochem., 41, 1504-1509.
Wyszkowska J. and Kucharski J., 2004. Biological properties of soil polluted with Chwastox Trio 540 SL (in Polish). Roczn. Glebozn., 50, 311-319.
Zantua M.J. and Bremner J.M., 1975. Comparison of methods of assaying urease activity in soils. Soil Biol. Biochem., 7, 291-295.