3D-analysis of plant microstructures: advantages and limitations of synchrotron X-ray microtomography

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Abstract

Synchrotron X-ray computer microtomography was used to analyze the microstructure of rose peduncles. Samples from three rose cultivars, differing in anatomy, were scanned to study the relation between tissue structure and peduncles mechanical strength. Additionally, chlorophyll fluorescence imaging and conventional light microscopy was applied to quantify possible irradiation-induced damage to plant physiology and tissue structure. The spatial resolution of synchrotron X-ray computer microtomography was sufficiently high to investigate the complex tissues of intact rose peduncles without the necessity of any preparation. However, synchrotron X-radiation induces two different types of damage on irradiated tissues. First, within a few hours after first X-ray exposure, there is a direct physical destruction of cell walls. In addition, a slow and delayed destruction of chlorophyll and, consequently, of photosynthetic activity occurred within hours/ days after the exposure. The results indicate that synchrotron X-ray computer microtomography is well suited for three-dimensional visualization of the microstructure of rose peduncles. However, in its current technique, synchrotron X-ray computer microtomography is not really non-destructive but induce tissue damage. Hence, this technique needs further optimization before it can be applied for time-series investigations of living plant materials

Abramoff M.D., Magelhaes P.J., and Ram S.J., 2004. Image processing with ImageJ. Biophotonics Int., 11, 36-42.

Banhart J., Borbély A., Dzieciol M., Manke I., Kardjilov N.,Kaysser-Pyzalla A.R., Garcia-Moreno F., Strobl M., andTreimer W., 2010. X-ray and neutron imaging - complementary techniques for materials science and engineering. Int. J. Mat. Res., 9, 1069-1079.

Bonse U. and Hart M., 1965. An X-ray interferometer. Appl. Phys. Lett., 6, 155-156.

Bouchard J., Méthot M., and Jordan B., 2006. The effects of ionizing radiation on the cellulose of woodfree paper. Cellulose, 13, 601-610.

Cherezov V., Riedl K.M., and Caffrey M., 2002. Too hot to handle? Synchrotron X-ray damage of lipid membranes and mesophases. J. Synchrotron Radiat., 9, 333-341.

Chunping Y., Zhiqiang S., Gucose Y., and Jianling W., 2008. Effect and aftereffect of ã radiation pretreatment on enzymatic hydrolysis of wheat straw. Biores. Technol., 99, 6240-6245.

Cloetens P., Mache R., Schlenker M., and Lerbs-Mache S.,2006. Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network. Proc. Nat. Acad. Sci. USA, 103, 14626-14630.

Cloetens P., Pateyron-Salomé M., Buffière J., Peix G.,Baruchel J., Peyrin F., and Schlenker M., 1997. Observation of microstructure and damage in materials by phase sensitive radiography and tomography. J. Appl. Phys., 81, 5878-5886.

Dhondt S., Vanhaeren H., van Loo D., Cnudde V., and Inzé D.,2010. Plant structure visualization by high-resolution X-ray computed tomography. Trends Plant Sci., 15, 419-433.

Driscoll M., Stipanovic A., Winter W., Cheng K., Manning M.,Spiese J., Galloway R.A., and Cleland M.R., 2009.

Electron beam irradiation of cellulose. Rad. Phys. Chem., 78, 539-542.

Garman E.F., 2010. Radiation damage in macromolecular crystallography: what is it and why should we care? Acta Cryst., 66, 339-351.

Graf W., 2010. Einfluss von Funktion und Struktur des Blütenstiels auf die Nacherntephase von Schnittrosen. Dissertation. de - Verlag im Internet GmbH, Berlin, Germany.

Kim H.K. and Lee S.J., 2010. Synchrotron X-ray imaging for nondestructive monitoring of sap flow dynamics through xylem vessel elements in rice leaves. New Phytol., 188, 1085-1098.

Matsushima U., Kardjilov N., Hilger A., Manke I., Shono H.,and Herppich W.B., 2009. Visualization of water usage and photosynthetic activity of street trees exposed to 2 ppm of SO2 - a combined evaluation by cold neutron and chlorophyll fluorescence imaging. Nucl. Instrum. Meth. A, 605, 185-187.

Mendoza F., Verboven P., Mebatsion H.K., Kerckhofs G.,Wevers M., and Nicolaï B., 2007. Three-dimensional pore space quantification of apple tissue using X-ray computed microtomography. Planta, 226, 559-570.

Mirone A., Wilcke R., Hammersley A., and Ferrero C., 2010. PyHST - High Speed Tomographic Reconstruction, http:// www.esrf.eu/UsersAndScience/Experiments/TBS/SciSoft.

Momose A., Takeda T., Itai Y., and Hirano K., 1996. Phasecontrast X-ray computed tomography for observing biological soft tissues. Nature Med., 2, 473-475.

Price L. and Klein W.H., 1962. Chlorophyll synthesis in X-irradiated etiolated bean leaf tissue. Rad. Bot., 1, 269-275.

Rack A., Zabler S., Müller B.R., Riesemeier H., WeidemannG.,Lange A., Goebbels J., Hentschel M., and Görner W.,2008. High resolution synchrotron-based radiography and tomography using hard X-rays at the BAMline (Bessy II).Nucl. Instrum. Meth. A, 586, 327-344.

Schmidt J.A., Rye C.S., and Gurmagui N., 1995. Lignin inhibits autoxidative degradation of cellulose. Polymer Degrad. Stabil., 49, 291-297.

Simonis W. and Seuberling H.B., 1973. Sensitivity of chlorophyll formation and of increase of NADP-dependent GDP-activity in greening Euglena gracilis to X-ray and other inhibitors of protein synthesis. Rad. Bot., 13, 297-300.

von Willert D.J., Matyssek R., and Herppich W.B., 1995. Experimentelle Pflanzenökologie: Grundlagen und Anwendungen. Georg Thieme Verlag, Stuttgart, Germany.

Weik M., Ravelli R.G., Kryger G., McSweeney S., Raves M.L.,Harel M., Gros P., Silman I., Kroon J., and Sussman J.L.,2000. Specific chemical and structural damage to proteins produced by synchrotron radiation. Proc. Nat. Acad. Sci., USA, 97, 623-628.

Zill L.P. and Tolbert N.E., 1958. The effects of ionizing and ultraviolet radiations on photosynthesis. Arch. Biochem. Biophys., 76, 196-203.

International Agrophysics

The Journal of Institute of Agrophysics of Polish Academy of Sciences

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