Stress responses of spring rape plants to soil flooding

Open access

Abstract

Stress responses of spring rape to soil hypoxia were investigated during 8-days flooding. Soil air-filled porosity decreased from 25-30% to 0%, oxygen diffusion rate - from 2.6-3.5 to 0.34 μmol O2 m-2 s-1, and redox potential - from 460 to 150mVwithin few hours. Alcohol dehydrogenase activity in roots increased up to 7-fold after one day of flooding and then decreased to 170% of control. Superoxide dismutase activity in roots increased by 27% during first 3 days and then dropped to 60% of control; in the leaves superoxide dismutase activity increased in average by 44%. Ascorbate peroxidase activity in leaves increased by 37% during first 3 days and then decreased to control value. Glutathione reductase activity increased by 45% in roots of flooded plants but did not change in leaves. Proline concentration in leaves increased up to 4-fold on the 3d day of flooding and then decreased to control value. Thus soil flooding induces increase of alcohol dehydrogenase activity and subsequent increase of superoxide dismutase and glutathione reductase activities in roots while the leaves display a few days increase of free proline concentration and ascorbate peroxidase activity, and a long-term increase of superoxide dismutase activity.

ArbonaV.,HossainZ.,López-ClimentM.F.,Pérez-Clemente R.M., and Gómez-Cadenas A., 2008. Antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus. Physiol. Plant., 132, 452-466.

Asada K., 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol., 141, 391-396.

Bai T.H., Li C.Y., Ma F.W., Shu H.R., and Han M.Y., 2008. Physiological responses and analysis of tolerance of apple rootstocks to root-zone hypoxia stress. Sci. Agric. Sinica, 41, 4140-4148.

Bailey-Serres J. and Voesenek L.A.C.J., 2008. Flooding stress: acclimations and genetic diversity. Ann. Rev. Plant Biol., 59, 313-339.

Balakhnina T., Bennicelli R., Stêpniewska Z., Stêpniewski W., and Fomina I., 2010. Oxidative damage and antioxidant defense system in leaves of Vicia faba major L. cv. Bartom during soil flooding and subsequent drainage. Plant Soil, 327, 293-301.

Balakhnina T.I.,GavrilovA.B.,W³odarczyk T.M., Borkowska A., Nosalewicz M., and Fomina I.R., 2009. Dihydroquercetin protects barley seeds against mould and increases seedling adaptive potential under soil flooding. Plant Growth Regul., 57, 127-135.

Bates L.S., Waldren R.P., and Teare I.D., 1973. Rapid determination of free proline for water-stress studies. Plant Soil, 39, 205-207.

Benz B.R., Rhode J.M., and Cruzan M.B., 2007. Aerenchyma development and elevated alcohol dehydrogenase activity as alternative responses to hypoxic soils in the Piriqueta caroliniana complex. Amer. J. Bot., 94, 542-550.

Blokhina O., Virolainen E., and Fagerstedt K.V., 2002. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals Bot., 91, 179-194.

Chen H. and Qualls R.G., 2003. Anaerobic metabolism in the roots of seedlings of the invasive exotic Lepidium latifolium. Environ. Exp. Bot., 50, 29-40.

Colmer T.D., 2003. Long-distance transport of gases in plants: a perspective on internal aeration and radial oxygen loss from roots. Plant Cell Environ., 26, 17-36.

Devkota A. and Jha P.K., 2011. Influence of water stress on growth and yield of Centella asiatica. Int. Agrophys., 25, 211-214.

Garnczanska M., 2002. Hypoxic induction of alcohol and lactate dehydrogenases in lupine seedlings. Acta Physiol. Plant., 24, 265-272.

Giannopolitis C.N. and Ries S.K., 1977. Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol., 59, 309-314.

Gliñski J. and Stepniewski W., 1985. Soil Aeration and Its Role for Plants. CRC, Boca Raton, FL, USA.

Lucassen E.C.H.E.T., Bobbink R., Smolders A.J.P, van der Ven P.J.M., Lamers L.P.M., and Roelofs J.G.M., 2002. Interactive effects of low pH and high ammonium levels responsible for the decline of Cirsium dissectum (L.) Hill. Plant Ecol., 165, 45-52.

Malicki M. and Walczak R.A., 1983.Agauge of the redox potential and the oxygen diffusion rate in the soil with an automatic regulation of cathode potential (in Polish). Zesz. Probl. Post. Nauk Roln., 220, 447-451.

Pederson O., Rich S.M., and Colmer T.D., 2009. Surviving floods: leaf gas films improve O2 and CO2 exchange, root aeration, and growth of completely submerged rice. Plant J., 58, 147-156.

Preiszner J., Vantoai T.T., Huynh L., Bolla R., and Yen H., 2001. Structure and activity of a soybean ADH promoter in transgenic hairy roots. Plant Cell Reports, 20, 763-769. Radyukina N.L., Shashukova A.V., Shevyakova N.I., and Kuznetsov V.V., 2008. Proline involvement in the common sage antioxidant system in the presence of NaCl and paraquat. Russian J. Plant. Physiol., 55, 649-656.

Rocha M., Licausi F., Araújo W.L., Nunes-Nesi A., Sodek L, Fernie A.R., and van Dongen J.T., 2010. Glycolysis and the tricarboxylic acid cycle are linked by alanine aminotransferase during hypoxia induced by waterlogging of Lotus japonicus. Plant Physiol., 152, 1501-1513.

Shevyakova N.I., Bakulina E.A., and Kuznetsov V.V., 2009. Proline antioxidant role in the common ice plant subjected to salinity and paraquat treatment inducing oxidative stress. Russian J. Plant Physiol., 56, 663-669.

Smith K.A. and Restall S.W.F., 2006. The occurrence of ethylene in anaerobic soil. Eur. J. Soil Sci., 22, 430-443.

Vartapetian B.B., Andreeva I.N.,Generozova I.P., Polyakova L.I., Maslova I.P., Dolgikh Yu.L., and Stepanova A.Yu., 2003. Functional electron microscopy in studies of plant response and adaptation to anaerobic stress. Annals Bot., 91, 155-172.

Wang K. and Jiang Y., 2007. Antioxidant responses of creeping bentgrass roots to waterlogging. Crop Sci., 47, 232-238.

Wignarajah K., Greenway H., and John C.D., 2010. Effect of waterlogging on growth and activity of alcohol dehydrogenase in barley and rice. New Phytol., 77, 585-592.

Yordanova L.Y. and Popova L.P., 2007. Flooding induced changes in photosynthesis and oxidative status in maize plants. Acta Physiol. Plant., 29, 535-541.

International Agrophysics

The Journal of Institute of Agrophysics of Polish Academy of Sciences

Journal Information


IMPACT FACTOR 2017: 1.242
5-year IMPACT FACTOR: 1.267

CiteScore 2017: 1.38

SCImago Journal Rank (SJR) 2017: 0.435
Source Normalized Impact per Paper (SNIP) 2017: 0.849

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 88 88 5
PDF Downloads 59 59 6