Fractal morphology of Beta vulgaris L. cell suspension culture permeabilized with Triton X-100®
In this work, morphology of Beta vulgaris L. cells permeabilized with 0.7mM of Triton X-100® was evaluated using digital image processing and concepts of fractal dimension (perimeter- area relations). Important morphometric changes were found when the contact-time with chemical agent was increased. The size of cells decreased, the cells lost the roundness and their shape was more sinuous; this behaviour was a result of a probable shrinkage caused by the excess of exposure with the permeabilization agent. Morphology of B. vulgaris cells after permeabilization, exhibited a fractal nature since the slope of the ratio of the logarithm of the perimeter vs logarithm of the area was higher than unit. Fractal geometry of the cell morphology was affected as a result of the exposure to Triton X-100®. Those changes can be attributed to the loss of turgor and structure of the cell wall.
Alamilla-Beltrán L., Chanona-Pérez J. J., Jiménez-Aparicio A. R., and Gutiérrez-López G. F., 2005. Description of morphological changes of particles alone spray drying. J. Food Eng., 67, 179-184
Bassetti L., Hagendoorn M., and Tramper J., 1995. Surfactant-induced non lethal release of anthaquinones from suspension cultures of Morinda citrifolia. J. Biotechnol., 39, 149-155.
Boitel-Conti M., Gontier E., Laberche J. C., Ducrocq C., and Sangwan-Norreel B. S., 1996. Inducer effect of Tween 20 permeabilization treatment used for release of stored tropane alkaloids in Datura innoxia Mill hairy root cultures. Plant Cell Reports, 16, 241-244.
Brodelius P., 1988. Permeabilization of plant cells for release of intracellularly stored products: viability studies. Appl. Microbiol. Biotechnol., 27, 561-566.
Buczkowski S., Hildgen P., and Cartilier L., 1998. Measurements of fractal dimension by box counting: a critical analysis of data scatter. Physica A, 252, 23-34.
Chanona J. J., Alamilla B. L., Farrera R. F., Quevedo R., Aguilera J. M., and Gutiérrez L. G., 2003. Description of the convective air-drying of a food model by means of the fractal theory. Int. J. Food Sci. Technol., 9, 207-213.
Gamborg O. L., Miller R. A., and Ojima K., 1968. Nutrient requeriment of suspension cultures of soybean root cells. Exp. Cell Res., 50, 151-158.
Hagiwara T., Wang H., Susuki T., and Takai R., 2002. Fractal analysis of ice crystals in frozen food. J. Agric. Food Chem., 50, 3085-3089
Ibaraki Y. and Kenji K., 2001. Application of image analysis to plant cell cultures. Computers Electronics Agric., 30, 193-203.
Kang M., Zeng Y., and Liu J., 2000. Fractal research on red blood cell aggregation. Clinical Hemorheology Microcirculation, 22, 229-236.
Kubík L. and Nozdrovicky L., 2005. Determination of the influence of tillage on the soil structure by fractal analysis. Int. Agrophysics, 19, 223-230.
Lerner H., Ben-Bassat D., Reinhold L., and Poljakoff-Mayber A., 1978. Induction of "pore" formation in plant cell membranes by toluene. Plant Physiol., 61, 213-217.
Martínez-López F., Cabrerizo M., and Hidalgo R., 2002. A study of the different methods usually employed to compute the fractal dimension. Physica A, 311, 411-428.
Meiners S., Gharyal P. K., and Schindler M., 1991. Permeabilization of the plasmalemma and wall of soybean root cells to macromolecules. Plant, 184, 443-447.
Miyanaga K., Seki M., and Furusaki S., 2000. Analysis of pigment accumulation heterogeneity in plant cell population by image-processing system. Biotechnol. Bioeng., 67, 493-497.
Mukundan U., Bhide V., Singh G., and Curtis W. R., 1998. pH-mediated release of betalains from transformed root cultures of Beta vulgaris L. Appl. Microbiol. Biotechnol., 50, 241-245.
Park Ch-H. and Martinez C., 1992. Enhanced release of rosmarinic acid from Coleus blumei permeabilized by dimethyl sulfoxide (DMSO) while preserving cell viability and growth. Biotechnol. Bioen., 40, 459-464.
Pepin M. F., Smith M. A. L., and Feid J. F., 1999. Application of imaging tools to plant cell culture: relationshipe between plant cell aggregation and flavonoid production. In vitro Cellular Develop. Biology, Plant, 35, 290-295.
Rito-Palomares M., 2008. Bioseparation: The limiting step in bioprocess development. J. Chem. Technol. Biotechnol., 83, 115-116.
Rodríguez M. M., Trejo E. J., Jiménez A. A., Morante M. L., Villarreal M., and Trejo T. G., 2004. Evaluation of morphological properties of Solanum chrysutrichum cell cultures in a shake flasks and fermentor and rheological properties of the broths. Food Technol. Biotechnol., 42, 153-158.
Sowana D. D., Williams D. R. G., O'Nelly B. K., and Dunlop E. H., 2002. Studies of the shear protective effects of pluronic F-68 on wild carrot cell cultures. Biochem. Eng. J., 12, 165-173.
SPSS, 1999. SigmaScan Pro. Users manual, SPSS Sci. Press, New York, USA.
Thimmaraju R., Bhagyalakshmi N., and Ravishankar G. A., 2004. In situ and ex situ adsorption and recovery of betalains from hairy root cultures of Beta vulgaris L. Biotechnol. Progress, 20, 777-785
Trejo-Tapia G., Cuevas-Celis J., Salcedo-Morales G., Trejo-Espino J. L., Arenas-Campo M. L., and Jiménez-Aparicio A., 2007.B. vulgaris L. suspension cultures permeabilized with Triton-100 retain cell viability and betacyanines production ability: A digital images analysis study. Biotechnol. Progress, 23, 359-363.
Verpoorte R., Contin A., and Memelink J., 2002. Biotechnology for the production of plant secondary metabolites. Phytochem., 1, 13-25.
Waliszewski P. and Konarski J., 2001. Tissue as self-organizing system with fractal dynamics. Adv. Space Res., 28, 545-548.
Yokota T., Tutumi N., and Takahashi K., 1999. Growth rate estimation of in vitro primarily induced carrot callus by a fractal based model. Biochem. Eng. J., 3, 231-234.