Nitrogen (N) is an essential macronutrient for wheat production. Soils can supply a considerable amount of N to a wheat crop as a result of mineralisation of N from the soil organic matter, a process that takes place both prior to and during the crop growth period (Sylvester-Bradley et al., 2001). However, under the high yielding conditions experienced in Ireland, application of fertilizer N is normally necessary to supplement available soil N to fulfil the crop’s requirement. For both economic and environmental reasons, efficient use of N is critical and matching N supply with crop demand, in both space and time, is an effective means of achieving efficient use of N (Zebarth and Rosen, 2007; Cui et al., 2010). Soil N is normally sufficient to supply a crop’s needs over the winter period and recovery of fertilizer N applied in the autumn/winter period is generally low; consequently, yield response to these applications is often low (Ellen and Spiertz, 1980; Powlson et al., 1986). As temperatures increase in spring, crop growth, and hence the crop N demand, increases such that soil N is no longer sufficient to supply the crop’s needs. If the soil inorganic N pool is not supplemented with fertilizer N, crop growth and yield will be reduced. The stage at which soil N becomes limiting, and therefore when fertilizer N is required, depends on the amount of N in the soil and, therefore, the optimum timing of the initial application of N is likely to be influenced by the levels of available soil N.
In addition to being available for crop uptake, inorganic N in the soil, originating either from fertilizers or from mineralisation of soil N, is subject to the process of immobilisation. Normally, levels of inorganic N decline relatively quickly (within 2–3 wk) after fertilizer N application as a result of either crop uptake or immobilisation (Recous and Machet, 1999). Therefore, applying fertilizer N in excess of the crop’s immediate requirement can result in a proportion of that N becoming unavailable to the crop through immobilisation, leading to reduced recovery of fertilizer N by a crop (King et al., 2001). This indicates that making the first application of N to a winter wheat crop too early, while the crop can still acquire adequate N from soil reserves, could lead to inefficient recovery of that fertilizer N by the crop.
A number of studies have found reduced recovery of N applied at tillering compared to that applied later in the growth cycle (Recous et al., 1988; Limaux et al., 1999; Blankenau et al.,2002; Howard et al., 2002). Therefore, applying fertilizer N when crop growth rates are such that there is a high demand for N increases the recovery of fertilizer N in the crop and also the grain yield (Fischbeck et al., 1990; Limaux et al., 1999). Consequently, N from fertilizer applications that are applied later in the growth cycle will be more efficiently recovered by a crop than earlier applications (Recous and Machet, 1999). Petersen (2004) concluded that the increased recovery of fertilizer N with delayed application was due to increased crop demand, which was linked to greater rates of leaf area production.
However, high rates of fertilizer N recovery are only advantageous if that N is efficiently converted into yield. Yield is closely related to intercepted solar radiation, which in turn is related to the amount of photosynthetic area of the crop (Gallagher and Biscoe, 1978). The photosynthetic area in turn is related to the N accumulation of the crop, so that N deficiency during the period of green area production can lead to reductions in yield (Sylvester-Bradley et al., 1990; Grindlay et al., 1997). Therefore, although delaying N fertilizer application may improve fertilizer N recovery in the crop, there may be adverse effects on aspects of the crop necessary for production of yield, such as the production of leaf area and number of grains per unit area during the early growth stages (GSs). Deciding on the date to initiate fertilizer N application can involve a trade-off between N recovery and efficiency of use of accumulated N in terms of yield production.
In Ireland, the current recommended timing for the first application of fertilizer N to winter wheat is between late tillering and the onset of stem elongation (Coulter and Lalor, 2008). In practice, many growers apply N in late February/early March, often in advance of significant spring growth, which may be leading to reduced nitrogen use efficiency (NUE). Fischbeck et al. (1990) indicated that excess N applications before the wheat crop began stem extension, particularly in areas where there was high N supply from the soil, could lead to reductions in yield. Relatively little work has been carried out regarding the optimum timing of the initial application of fertilizer N to wheat under Irish conditions. Fewer (2002) reported that there was no significant effect on grain yield when delaying the initial application to winter wheat until GS 31, where the wheat was grown after a grass ley and where N supply from the soil in spring is likely to have been high. However, a significant reduction in yield was observed when the initial N was delayed until GS 31 when the wheat crop was grown after three years of arable cropping, where N supply from the soil was lower than that from the site following a grass ley. This suggests that the level of soil N supply in early spring may be important when deciding the stage at which to initiate fertilizer N application. In Belgian conditions, Bodson et al. (2001) concluded that in many situations, delaying the initial N application until GS30 had no significant effect on grain yield, but in certain situations, particularly in which root growth was restricted, delaying the initial N could cause yield reduction. The objectives of this work were: (a) to determine the effects of initiating fertilizer N application to winter wheat at different GSs on grain yield and NUE of the fertilizer N and (b) to determine whether plant population density or soil N supply potential influenced the stage at which the first N application should be applied.
Materials and methods
Two field experiments were carried out at the Teagasc Crops Research Centre, Oak Park, Carlow, Ireland (52.86° N, 6.92° W, 54 m above mean sea level): one in the 2010/11 growing season and one in the 2011/12 growing season (henceforth referred to as 2011 and 2012, respectively). The soil at both sites is a grey–brown Podzolic of the Mortarstown Series (Conry and Ryan, 1967). Both sites had been cultivated for at least 20 yr prior to the initiation of the experiments; the previous crops were winter oats and winter oilseed rape for the 2011 and 2012 experiments, respectively.
Experimental layout, design and treatments
A split-plot experimental arrangement, laid out in a randomised complete block design with four replications, was used for both experiments. To facilitate repeated destructive sampling, each split plot comprised two adjacent plots (12 m × 2.15 m), one of which was designated for destructive sampling and the other reserved for yield determination. In both seasons, each plot had 14 rows spaced 15.4 cm apart.
The main plot factor was slurry treatment; there were two levels (with and without slurry). The slurry treatment was included to simulate a higher level of soil N supply (SNS) compared to the treatment in which slurry was not applied. Cattle slurry was applied, using a tractor-drawn trailing hose tanker system, to the stubbles of the previous crop and immediately (within 1 h) incorporated by ploughing. Total N applied in the slurry (22 m3/ha containing 2.35 kg N/m3) was 51.7 kg/ha in 2011 and 2012. Sowing of the wheat crop took place within 2 d of slurry application in both seasons. To ensure that any effect of the slurry would be mainly due to its effect on SNS to the crop, P and K fertilizer was applied to the no-slurry plots to balance the estimated P and K inputs in the slurry to the slurry-treated plots.
The split-plot treatments included a factorial arrangement of N regime (five fertilizer N application timing treatments and an unfertilized control [0N]) and seed rate (high and low). Seed rate was included as a factor to give variation in plant population density. The seed rates used were 400 seeds/m2 and 100 seeds/m2, to give plant population densities sufficient to saturate the yield response to increasing seed rate and a suboptimal plant population density, respectively (Spink et al., 2000).
The N application timing treatments were defined by the GS (Zadoks et al., 1974) at which the first of a total of two applications of fertilizer N was applied. Five fertilizer N application timing treatments, where the first N fertilizer was applied at GSs 24–26, 30, 31, 32 or 37 (hereafter referred to as Ntillering, N30, N31, N32 and N37) were included (Table 1). A control treatment receiving no fertilizer N (0N) was also included. Each treatment received 70 kg N/ha in the first fertilizer application. A second application of 130 kg N/ha was made 15–27 d after the first application but not before GS 31 in the case of Ntillering and N30 to prevent excessive N applications before significant crop growth occurred in these treatments. Calcium ammonium nitrate was used as the N source. Fertilizer was applied using a carefully calibrated modified seed drill (Fiona Maskinfabrik A/S, Bogense, Syddanmark, Denmark).
Dates and growth stages at which fertilizer N was applied
|First application||Second application|
|N application timing||Date||GS1||Date||GS1|
|Ntillering||Mar 9||GS 26||Apr 11||GS 31|
|N30||Mar 28||GS 30||Apr 15||GS 31|
|N31||Apr 11||GS 31||Apr 27||GS 33|
|N32||Apr 20||GS 32||May 10||GS 39|
|N37||May 10||GS 39||May 24||GS 55|
|Ntillering||Feb 29||GS 24||Apr 4||GS 31|
|N30||Mar 23||GS 30||Apr 19||GS 32|
|N31||Apr 4||GS 31||Apr 19||GS 32|
|N32||Apr 19||GS 32||May 4||GS 32|
|N37||May 14||GS 37||May 25||GS 51|
Winter wheat (cv. Cordiale) was sown on 12 October 2010 and 14 October 2011. Herbicides, fungicides and insecticides were applied uniformly across the trials according to standard farm practice to maintain the crop free of weeds, fungal disease and insect pests. Nutrients, other than nitrogen, were applied uniformly to the experiments according to the requirements of the crop (Coulter and Lalor, 2008).
Sample collection and analysis
Samples were taken on three occasions in each season: at GS 30 (26 March 2011, 30 March 2012), at anthesis (GS 61-65; 12 June 2011, 18 June 2012) and at maturity (9 August 2011; 13 August 2012), just prior to combine harvest. At GS 30, samples were only collected from the unfertilized treatment. All plots were sampled at anthesis and maturity. On each occasion, plants from two adjacent 0.5 m row lengths at two random locations (equivalent to a sampling area of 0.308 m2) were sampled. Roots were removed and samples were dried in a force-ventilated oven for 3 d at 70°C and dry matter (DM) yield determined.
At maturity, prior to oven-drying, the total number of ears in each sample was determined and expressed as ears/m2. Samples were then threshed, using a laboratory threshing machine, and the straw, grain and chaff were collected separately. Thousand grain weight (TGW) was determined for each sample using an electronic grain counter (Contador; Pfeuffer, Kitzingen, Germany) and expressed at 85% DM after oven-drying. The mean number of grains per ear (GPE) was calculated by dividing the total number of grains in each sample by the number of ears in each sample.
Samples were subsequently milled (< 2 mm particle size) using a laboratory grinder (SM100; Retsch, Haan, Germany) and total N concentration determined using a Leco FP 328 instrument (LECO Corporation, St. Joseph, MI, USA) calibrated using an ethylenediaminetetraacetic acid standard. Total N accumulation for each sampling date was calculated as the product of DM yield and the N concentration (grain N concentration was calculated from grain protein concentration using a conversion factor of 5.7). At harvest, the N accumulation levels for straw, chaff and grain were summed up to give the total N accumulation.
At maturity, grain yield was determined using a Deutz-Fahr combine harvester modified for plot harvesting with on-board electronic weighing equipment. A subsample of grain (~ 2 kg) was taken from each plot, and moisture and crude protein contents were determined using a near infra-red spectroscopy instrument (Infratec 1241; Foss A/S, Hillerød, Denmark). Grain yield (t/ha) was expressed at 15% moisture.
Efficiency ratios of fertilizer N use for the harvest sampling date were calculated according to Ladha et al. (2005).
Recovery efficiency of N (REN) = (UF - U0)/FN
Agronomic efficiency of N (AEN) = (YF - Y0)/FN
Physiological efficiency of N (PEN) = (YF - Y0)/(UF - U0)
where UF and U0 are the total N accumulation levels (kilograms N per hectare) and YF and Y0 are the grain yields (kilograms dry matter per hectare) in the fertilized and unfertilized treatments, respectively, and FN is the fertilizer N application rate (kilograms N per hectare).
A combined analysis of variance over the two years was carried out for each variable. Data were analysed using the MIXED procedure of SAS 9.1 (SAS, 2004). Year, seed rate, slurry application, N regime and their interactions were included as fixed effects; replication and replication × slurry were included as random effects. When significant (P < 0.05) effects of the fixed effects or their interactions were found, least-square means were separated using the Tukey–Kramer multiple comparison test.
Grain yield and yield components
Grain yield in the 0N samples did not differ significantly between the two years (Figure 1). For the fertilized treatments, yields in the Ntillering, N30 and N31 samples were significantly higher in 2011 than in 2012, while the yields in N32 and N37 did not differ significantly between years.
All fertilized treatments had significantly higher yield than the 0N sample in both years (Figure 1). N application timing had a significant effect on grain yield, although the effects of N application timing varied between years, as indicated by a significant interaction between N regime and year (Table 2). In 2011, delaying the first N application until GS 30 or GS 31 gave similar yields, compared to the yields after first N application at tillering. Delaying the first application of fertilizer N until GS 31 caused a significant reduction in yield (0.7 t/ha) compared to that obtained after first N application at GS 30. However, yield at N31 was not significantly different from that at Ntillering. Each delay in the first N application after GS 31 caused a significant reduction in yield of at least 0.9 t/ha. In 2012, similar yields were achieved irrespective of whether the first N application was at tillering, GS30, GS 31 or GS32. A significant reduction (0.9 t/ha) was observed when the first N application was delayed from GS32 to GS37 (Figure 1). N37 gave the lowest yield in both years. It was noted that tillers that had not reached maturity were prevalent in the N37 treatment, but not in any other treatment, at harvest.
Tests of fixed effects for yield, components of yield, N accumulation and N efficiency ratios
|Effect||Yield||Components of yield||N accumulation||N efficiency ratio||Grain N concentration|
|N regime (N)||***||***||***||***||-||***||***||**||***||***||***|
|Seed rate (R)||***||***||NS||***||**||**||**||NS||**||*||***|
|N × Y||***||***||***||***||-||**||NS||NS||***||***||***|
|S × Y||NS||NS||*||NS||NS||NS||NS||NS||NS||NS||**|
|R × Y||NS||*||NS||**||NS||NS||NS||NS||*||*||**|
|N × S||NS||*||**||NS||-||NS||NS||NS||NS||NS||NS|
|N × R||NS||**||NS||NS||-||NS||NS||NS||NS||NS||NS|
|S × R||NS||NS||NS||*||NS||NS||NS||*||**||NS||NS|
|N × S × R||NS||NS||NS||NS||-||NS||NS||NS||NS||NS||NS|
|N × S × Y||NS||NS||NS||NS||-||NS||NS||NS||NS||NS||NS|
|N × R × Y||NS||NS||NS||***||-||NS||NS||NS||NS||NS||NS|
|S × R × Y||NS||NS||NS||NS||NS||NS||NS||NS||NS||*||NS|
|N × S × R × Y||NS||NS||NS||NS||-||NS||NS||NS||NS||NS||NS|
NS indicates non-significant results.
Correlation analysis indicated that yield differences between N application timing treatments in 2011 (i.e., excluding 0N) were due to the effects of the treatments on both GPE and TGW (Table 3). Ear density was not correlated (r = -0.20, P = 0.07) with yield in 2011. In 2012, differences in yield were due to differences in TGW and ear density, but the lower correlation coefficients indicated that the relationships were weaker than in 2011.
Effect of N regime on components of winter wheat grain yield in 2011 and 2012
|N regime||TGW (g)||GPE||Ears/m2|
|0N||50.4 bc||40.3 e||31.2 b||44.8 a||282.1 e||349.8 d|
|Ntillering||55.3 ab||40.5 e||48.0 a||45.6 a||444.2 bc||532.7 a|
|N30||55.0 a||40.2 e||50.3 a||47.4 a||429.4 c||519.3 a|
|N31||53.2 ab||40.7 de||50.0 a||48.6 a||405.0 cd||495.9 ab|
|N32||50.0 bc||39.8 e||45.6 a||49.4 a||435.1 bc||530.0 a|
|N37||46.4 dc||39.2 e||28.8 b||49.1 a||539.6 a||458.8 bc|
For each component of yield, means followed by the same letter are not significantly different.
There was a significant effect of seed rate on yield; increasing the seed rate from 100 seeds/m2 to 400 seeds/m2 gave a significant increase in mean yield of 0.36 t/ha (data not presented). No significant interaction between N regime and seed rate was detected, indicating that seed rate did not influence N regime effects (Table 2).
Slurry application had no significant effect on N accumulation in March, at anthesis or at harvest (Table 2). The higher seed rate gave significantly higher N accumulation at all three sampling dates, but the differences (9.3 kg N/ha, 9.2 kg N/ha and 8.5 kg N/ha in March, at anthesis and at harvest, respectively) were small. N accumulation was significantly greater both in March (15.6 kg N/ha in 2011 and 29.3 kg N/ha in 2012) and at anthesis in 2012 compared to the same periods in 2011, but there was no significant difference between the years at harvest.
Fertilizer addition, irrespective of timing of the first application, significantly increased N accumulation in both years at both anthesis and harvest (Figure 2). Differences were detected between the results at anthesis and harvest after different N application timings. At anthesis, the effect of N application timing was influenced by year. This reflected a greater reduction in N accumulation as the initial fertilizer N application was delayed in 2011 than in 2012 such that the difference in N accumulation between Ntillering and N37 was much greater in 2011 than in 2012.
At harvest, there was no significant interaction between N regime and any other effect in terms of N accumulation (Table 2). There was a trend towards increased N accumulation as first N application was delayed. However, there was no statistically significant difference in N accumulation at harvest between the first four N application timings; N accumulation for N37 was significantly higher than that for Ntillering (Figure 2).
Seed rate or slurry application did not influence the effect of N application timing on N accumulation at harvest.
N use efficiency
Effects of N application timing on N accumulation were mirrored in the REN, with a trend towards higher REN as the first N application was delayed, but a significant difference was only detected between N37 and Ntillering (Table 4). Effects of N application timing on REN were not significantly influenced by either seed rate or slurry application. REN did not differ significantly between years.
Effect of N application timing on recovery (REN), agronomic (AEN) and physiological (PEN) efficiency of N use
|REN1 (kg N/kg N)||AEN (kg DM/kg N)||PEN (kg DM/kg N)|
|Ntillering||60.1 a||25.2 ab||13.3 d||41.8 a||22.2 d|
|N30||65.8 ab||27.0 a||13.8 cd||40.8 ab||21.2 d|
|N31||63.7 ab||24.0 b||13.2 d||38.2 b||20.2 d|
|N32||65.5 ab||20.1 c||12.8 d||29.6 c||20.2 d|
|N37||69.5 b||13.2 d||9.1 e||18.2 de||13.3 e|
Means for an efficiency ratio followed by the same letter are not significantly different.
Slurry addition had no significant effect on REN (Table 2). REN was significantly lower at the lower seed rate, but not at the high seed rate, where slurry was applied.
Agronomic efficiency (AEN), the increase in yield relative to that of the unfertilized control per kilogram fertilizer N applied, was significantly affected by both N regime and year, but a significant interaction between N regime and year indicated that the effect of N regime varied between the two years (Table 2). AEN was significantly higher for all N application timings, except N37, in 2011 compared to 2012, reflecting the higher yields achieved in 2011 (Table 4). In 2011, differences in AEN were small among the first three N application timings, but declined significantly with each successive delay in initial fertilizer N application after GS 31. In 2012, there was no significant difference in AEN between the first four application times, but a significant decline occurred when the first N application was delayed from GS 32 to GS 37.
The effect of N application timing on AEN was not influenced by slurry application or seed rate (Table 2). Increasing seed rate significantly increased AEN in 2012 but not in 2011. The effect of seed rate was also influenced by slurry application; slurry application reduced AEN at the low seed rate but not at the high seed rate.
Slurry application had no significant effect on PEN (Table 2). The higher seed rate gave significantly higher PEN than the low seed rate in 2012 but there was no significant effect of seed rate in 2012. The effect of seed rate on PEN was small compared to the effect of fertilizer N application or year. N regime had a significant effect on PEN, but the effect was influenced by year, as indicated by a significant interaction. This was a result of the differences in the response of PEN to delays in fertilizer N application between the years. PEN for Ntillering was 41.8 kg DM/kg N in 2011 and declined as the first N application was delayed such that PEN for N37 was 18.2 kg DM/kg N (Table 4). In 2012, PEN for Ntillering was 22.2 kg DM/kg N, not significantly different from that for N37 in 2011, but significantly lower than that for Ntillering in 2011. In 2012, unlike in 2011, reduction in PEN as a result of delaying the initial application of N was much lower, with no significant differences detected between the first four N application timings.
Grain N concentration
N regime had a significant effect on grain N concentration, but the effect varied between years (Table 2; Figure 3). Fertilizer addition, irrespective of application timing, significantly increased grain N concentration in both years. Delaying the first application of N resulted in an increase in grain N concentration in both years, but the rate of increase differed between years. In 2011, each additional delay in first N application after GS 30 caused a significant increase in grain N concentration; there was no significant difference between Ntillering and N30. In 2012, the increase in grain N concentration was less pronounced than in 2011 when the first N application was delayed from the tillering stage to GS 32.
In terms of the initial application of fertilizer N to winter wheat, this study indicated that, compared to applying fertilizer N at tillering, there was no disadvantage to delaying the first N application until GS 31 in terms of yield. However, as indicated by the significant decrease in yield as the first N application was delayed from GS 30 to GS 31 in 2011, delaying the first N application until after GS 30 can increase the chances of yield loss. These results are in agreement with those of Bodson et al. (2001) in Belgium. Schulz et al. (2015) also found that delaying N application until the early stem elongation stage had no detrimental effect on yield under high-yielding German conditions.
The absence of a yield reduction as N application was delayed from tillering to GS 31 would indicate that either there was sufficient N in the soil to support the plant’s needs during this period or that the plant was able to overcome any temporary deficiency in N that occurred during this period. N deficiency during this early part of the growing season would be most likely to interrupt the tillering process and lead to death of the younger tillers (Masle, 1981, 1985). However, the lack of significant differences in ear population density between the first three N application timings would indicate that, even if tiller death did occur, it was not of sufficient magnitude to reduce the number of ear-bearing tillers surviving to harvest or that compensatory tiller production occurred once N was applied. The ability of the wheat plant to compensate for periods of deficiency early in the season was particularly evident in 2011 when the N37 treatment had the highest ear population density, despite not receiving fertilizer until after the stem elongation phase had been completed. This suggests that even where N is withheld, the crop still retains the ability to compensate by producing additional ear-bearing tillers. However, from a practical point of view, these late-appearing tillers were still immature when the main stem of the same plants had reached maturity, which would cause difficulties for harvesting the crop at the farm level.
Grain yield in wheat is better related to grain number per unit area than to individual grain weight (Slafer and Andrade, 1989). Nitrogen deficiency during the stem elongation phase is more likely to result in reduced GPE by reducing spikelet number or by increasing floret abortion (Langer and Liew, 1973; Whingwiri and Kemp, 1980; McMaster, 1997). There was some evidence that this occurred in 2011 considering that the GPE was reduced when the first application of N fertilizer was withheld until GS 37. This was not evident in 2012, perhaps due to a higher supply of N from the soil, as indicated by the higher N accumulation in samples from areas where no fertilizer N was applied.
The correlation between the reduction in yield encountered as a result of delaying the first N application and a reduction in grain weight in both years, but particularly in 2011, indicated that a restriction on the supply of photosynthate to the developing grain may have been the key effect of delayed N application. Two sources of photosynthate contribute to grain filling in wheat: current photosynthate and photosynthate produced prior to anthesis and stored in the vegetative tissues, particularly in the stem (Schnyder, 1993). Stem reserves of water-soluble carbohydrate can play an important role in supplying carbohydrate to the grain during grain filling, potentially contributing over two-thirds of the final grain weight in areas where stress conditions occur (Ehdaie et al., 2008). It is possible that stem reserves were diminished where the first application of fertilizer N was progressively delayed due to reduced canopy formation, and hence reduced photosynthesis, during the pre-anthesis stage.However, stem reserves have been found to be higher on a per-stem basis at anthesis in N-deficient wheat than in adequately fertilized wheat, with a corresponding higher grain weight for the N-deficient crop at harvest (van Herwaarden et al., 1998a, 1998b).
A more likely reason could be a reduction in current photosynthesis as a result of reduced leaf area where N application was delayed. Mossedaq and Smith (1994) found that delaying N application until anthesis, compared with applying N just prior to stem extension, caused a reduction in leaf area and therefore reduced assimilation capacity. Additionally, for the N37 treatment, the presence of late-emerging tillers that were harvested before maturity, and therefore, before the grains in those ears had reached their full weight, would have contributed to the lower grain weight in that treatment.
Both experiments in this work were carried out in areas where the risk of take-all caused by Gaeumannomyces graminis was low because the previous crop was a non-host of the fungus (Hornby, 1998). Take-all causes a progressive deterioration in root function as the season progresses, which affects nutrient accumulation negatively (Schoeny et al., 2003). This could indicate that delayed N application to crops infected with takeall could lead to reduced capture of that N and consequently reduced yield. Therefore, where take-all is likely to be present, earlier application of N may be beneficial, although Werker and Gilligan (1990) found no effect of altering N application timing on take-all dynamics.
The increase in REN of fertilizer N when the first application was delayed until GS 37, compared to making the first N application at tillering, is consistent with previous findings (Recous and Machet, 1999). However, this increased recovery coincided with a decline in both AEN and PEN due to the reduction in yield that occurred when N application was delayed. Therefore, there is a trade-off between REN and both AEN and PEN. Reducing the amount of N applied at the first application at tillering or early stem extension, and hence when crop demand would have been low, may have increased the REN without reducing AEN or PEN.
The N application timing treatments in this study were defined by timing of the initial application of N in a two-split approach, with the timing of the second application dictated by the timing of the first. Therefore, the whole fertilizer N regime was shifted in time, not just the initial N application, and it could be argued that the observed treatment effects may have been due, at least in part, to differences in timing of the second application. To avoid confounding the effect of timing of the first application with the timing of the second application, single applications of fertilizer N at the various GSs could have been used. However, studies have shown that applications of high amounts of N before stem extension, as would be the case for the tillering application in this study, can lead to reduced NUE and may increase the risk of lodging and loss of N by leaching (Maidl et al., 1998; Sticksel et al., 2000). In any case, at the farm level, where high amounts of N are being applied, a multiple-split approach is normally recommended (Coulter and Lalor, 2008; Department of the Environment, Food and Rural Affairs, 2010) even though comparisons of single applications with split applications of a given amount of fertilizer N have often shown no effect, positive or negative, on grain yield (Schulz et al., 2015). Therefore, whether the observed effects were due to the timing of the first or second application would be of little concern from a practical point of view.
The non-significant interaction between N regime and seed rate for both yield and ear populations means that there was no evidence found in this study to indicate that crops with a lower plant population required earlier N applications compared to crops with high plant populations. This suggests that there is little scope to manipulate crops with low plant density using the timing of the first N application. Spink et al. (2005) also found little evidence to support alterations in the timing of initiation of N fertilization in winter wheat based on plant population despite finding that earlier N application produced more shoots per unit area.
The absence of a significant effect of the pre-sowing slurry application on crop N accumulation in March, before the onset of spring growth, or at anthesis and harvest, where no fertilizer N was applied, indicates that the nitrogen contained in the slurry was of no net benefit to the crop. Poor recovery by winter cereals of N in autumn-applied slurries has been reported previously as a result of loss of the N from the soil profile during the period of low crop demand over the winter period (Smith and Chambers, 1993; Sieling et al., 1997). This lack of a slurry effect meant that it was not possible to determine objectively whether lower levels of SNS would require earlier applications of fertilizer N, to maintain yield, than situations wherein higher amounts of SNS were present. It is possible that at least some of the differences in the effect of N application timing on yield between the two years were due to differences in SNS. In 2012, when the SNS was higher than in 2011, as indicated by crop accumulation in the absence of fertilizer N, there was no difference in yield as the first N application was delayed from the tillering stage to GS 32. This may indicate that the crop did not experience sufficient levels of deficiency to affect either ear populations or GPE, the two components of yield most likely to be influenced by deficiency during that time. In 2011, when soil N supply was lower, as indicated by crop N accumulation, there was a negative effect on yield when the first N application was delayed until GS 31 compared to the result obtained on making the N application at GS 30. This may have been at least in part due to the higher soil N supply, but the differences cannot be conclusively linked to differences in soil N supply. In particular, the winter period preceding the 2012 growing season was milder and drier than that preceding the 2011 season (Anon, 2011, 2012), which may have resulted in greater amounts of N mineralised and/ or less N loss via leaching (Herlihy, 1979; White et al., 1983).
The results of these experiments indicate that, despite greater recovery of the applied fertilizer, in order to avoid yield reductions, the first N application to winter wheat should not be delayed after GS 30. Differences in yield between first N application at tillering, GS 30 and GS31 were dependent on season, with some indication that GS 30 may give the highest yield. Optimum timing of the initial application of fertilizer N was not influenced by plant population density. The work also indicated that autumn slurry application is not an effective strategy to supply N to winter wheat crops under Irish growing conditions.
This work was funded by the Teagasc Walsh Fellowship Scheme. We thank all the staff of Teagasc Crops Research Centre in Oak Park who have helped with laboratory and field work.
Anon. 2011. “Winter Weather 2011 Summary”. Met Éireann, Dublin, Ireland. Available online: http://www.met.ie/climate/monthly-weather-reports.asp [Accessed 17 September 2015].
Anon. 2012. “Winter Weather 2012 Summary”. Met Éireann, Dublin, Ireland. Available online: http://www.met.ie/climate/monthly-weather-reports.asp [Accessed 17 September 2015].
Blankenau, K., Olfs, H.W. and Kuhlmann, H. 2002. Strategies to improve the use efficiency of mineral fertilizer nitrogen applied to winter wheat. Journal of Agronomy and Crop Science 188: 146–154.
Bodson, B., Vancutsem, F. and Fallisse, A. 2001. Timing of nitrogen application to improve quality, reduce lodging and minimise disease risks for hybrid and inbred wheat. Aspects of Applied Biology 64: 193–200.
Conry, M.J. and Ryan, P. 1967. “Soils of Co. Carlow”. An Foras Taluntais, Dublin, 204 pages. Available online: https://www.teagasc.ie/media/website/environment/soil/book.pdf [Accessed 17 September 2015].
Coulter, B.S. and Lalor, S. 2008. “Major and Micro Nutrient Advice for Productive Agricultural Crops”. Teagasc, Johnstown Castle, Wexford, Ireland, 116 pages.
Cui, Z., Zhang, F., Chen, X., Dou, Z. and Li, J. 2010. In-season nitrogen management strategy for winter wheat: maximizing yields, minimizing environmental impact in an over-fertilization context. Field Crops Research 116: 140–146.
Department of the Environment, Food and Rural Affairs (DEFRA). 2010. “The Fertiliser Manual (RB209)”. Department of the Environment, Food and Rural Affairs, TSO (The Stationary Office), London, 252 pages.
Ehdaie, B., Alloush, G.A. and Waines, J.G. 2008. Genotypic variation in linear rate of grain growth and contribution of stem reserves to grain yield in wheat. Field Crops Research 106: 34–43.
Ellen, J. and Spiertz, J.H.J. 1980. Effects of rate and timing of nitrogen dressings on grain yield formation of winter wheat (T. aestivum L.). Fertilizer Research 1: 177–190.
Fewer, D. 2002. The effect of varying nitrogen fertilization programmes on the nitrogen economy, grain yield and quality of pureline and hybrid winter wheat. MSc. thesis, The National University of Ireland, Ireland.
Fischbeck, G., Dennert, J. and Muller, R. 1990. N-Dynamik des Bodens, Ertragsbildung und Stickstoffentzug von Winterweizen bei unterschiedlicher Hohe und Verteilung der mineralischen N-Dungung. Journal of Agronomy and Crop Science 164: 297–311.
Gallagher, J.N. and Biscoe, P.V. 1978. Radiation absorption, growth and yield of cereals. The Journal of Agricultural Science, Cambridge 91: 47–60.
Grindlay, D.J.C., Sylvester-Bradley, R. and Scott, R.K. 1997. The relationship between canopy green area and nitrogen in the shoot. In: “Diagnostic Procedures for Crop N Management” (eds. G. Lemaire and I.G. Burns), INRA, Paris, pages 53–60.
Herlihy, M. 1979. Nitrogen mineralisation in soils of varying texture, moisture and organic matter. Plant and Soil 53: 255–267.
Hornby, D. 1998. “Take-All of Cereals. A Regional Perspective”. CAB International, Wallingford, UK.
Howard, D., Newman, M., Essington, M. and Percell, W. 2002. Nitrogen fertilization of conservation-tilled wheat. II. Timing of application of two nitrogen sources. Journal of Plant Nutrition 25: 1329–1339.
King, J. A., Sylvester-Bradley, R. and Rochford, A. S. H. 2001. Availability of nitrogen after fertilizer applications to cereals. The Journal of Agricultural Science, Cambridge 136: 141–157.
Ladha, J.K., Pathak, H., Krupnik, T.J., Six, J. and van Kessel, C. 2005. Efficiency of fertilizer nitrogen in cereal production: retrospects and prospects. Advances in Agronomy 87: 86–156.
Langer, R.H.M. and Liew, F.K.Y. 1973. Effects of varying nitrogen supply at different stages of the reproductive phase on spikelet and grain production and on grain nitrogen in wheat. Australian Journal of Agricultural Research 24: 647–656.
Limaux, F., Recous, S., Meynard, J.M. and Guckert, A. 1999. Relationship between rate of crop growth at date of fertiliser N application and fate of fertiliser N applied to winter wheat. Plant and Soil 214: 49–59.
Maidl, F.X., Sticksel, E., Retzer, F. and Fischbeck, G. 1998. Effect of varied N-fertilization on yield formation of winter wheat under particular consideration of mainstems and tillers. Journal of Agronomy and Crop Science 180: 15–22.
Masle, J. 1981. Elaboration du nombre d’epis d’un peuplement de blé d’hiver en situation de compétition pour l’azote. 1. Mise en évidence d’un stade critique pour la montée d’une talle. Agronomie 8: 623–632.
Masle, J. 1985. Competition among tillers in winter wheat: consequences for growth and development of the crop. In: “Wheat Growth and Modelling” (eds. W. Day and R.K. Atkin), Plenum Press, New York, pages 33–54.
McMaster, G.S. 1997. Phenology, development and growth of the wheat (Triticum aestivum L.) shoot apex: a review. Advances in Agronomy 59: 63–118.
Mossedaq, F. and Smith, D.H. 1994. Timing nitrogen application to enhance spring wheat yields in a Mediterranean climate. Agronomy Journal 86: 221–226.
Petersen, J. 2004. Crop uptake of 15N labelled fertilizer in spring wheat affected by application time. Acta Agriculturae Scandinavica, Section B Soil & Plant Science 54: 83–90.
Powlson, D.S., Hart, P.B.S., Pruden, G. and Jenkinson, D.S. 1986. Recovery of 15N-labelled fertilizer applied in autumn to winter wheat at four sites in eastern England. The Journal of Agricultural Science, Cambridge 107: 611–620.
Recous, S. and Machet, J.M. 1999. Short-term immobilisation and crop uptake of fertiliser nitrogen applied to winter wheat: effect of date of application in spring. Plant and Soil 206: 137–149.
Recous, S., Fresneau, C., Fauric, G. and Mary, B. 1988. The fate of labelled 15N urea and ammonium nitrate applied to a winter wheat crop. I. Nitrogen transformations in the soil. Plant and Soil 112: 205–214.
SAS. 2004. Version 9.1.2, SAS Institute, Cary, NC, USA.
Schnyder, H. 1993. The role of carbohydrate storage and redistribution in the source-sink relations of wheat and barley during grain filling – a review. New Phytologist 123: 233–245.
Schoeny, A., Devienne-Barret, F., Jeuffroy, M.H. and Lucas, P. 2003. Effect of take-all root infections on nitrate uptake in winter wheat. Plant Pathology 52: 52–59.
Schulz, R., Makary, T., Hubert, S., Hartung, K., Gruber, S., Donath, S., Döhler, J., Weiß, K., Ehrhart, E., Claupein, W., Piepho, H.P., Pekrun, C. and Müller, T. 2015. Is it necessary to split nitrogen fertilization for winter wheat? On-farm research on Luvisols in SouthWest Germany. The Journal of Agricultural Science, Cambridge 153: 575–587.
Sieling, K., Günther-Borstel, O. and Hanus, H. 1997. Effect of slurry application and mineral nitrogen fertilization on N leaching in different crop combinations. The Journal of Agricultural Science, Cambridge 128: 79–86.
Slafer, G.A. and Andrade, F.H. 1989. Genetic improvement in bread wheat (Triticum aestivum) yield in Argentina. Field Crops Research 21: 289–296.
Smith, K.A. and Chambers, B.J. 1993. Utilizing the nitrogen content of organic manures on farms – problems and practical solutions. Soil Use and Management 9: 105–111.
Spink, J.H., Semere, T., Sparkes, D.L., Whaley, J.M., Foulkes, M.J., Clare, R.W. and Scott, R.K. 2000. Effect of sowing date on optimum plant density of winter wheat. Annals of Applied Biology 137: 179–188.
Spink, J.H., Berry, P., Theobald, C., Sparkes, D., Wade, A.P. and Roberts, A. 2005. “The Effect of Location and Management on the Target Drilling Rate for Winter Wheat”. HGCA Project Report No. 361. Available online: https://cereals.ahdb.org.uk/media/272086/pr361-pg-1-32.pdf [Accessed 17 September 2015].
Sticksel, E., Maidl, F.X., Retzer, F., Dennert, J. and Fischbeck, G. 2000. Efficiency of grain production of winter wheat as affected by N fertilisation under particular consideration of single culm sink size. European Journal of Agronomy 13: 287–294.
Sylvester-Bradley, R., Stokes, D.T., Scott, R.K. and Willington, V.B.A. 1990. A physiological analysis of the diminishing responses of winter wheat to applied nitrogen. 2. Evidence. Aspects of Applied Biology 25: 289–300.
Sylvester-Bradley, R., Stokes, D.T. and Scott, R.K. 2001. Dynamics of nitrogen capture without fertilizer: the baseline for fertilizing winter wheat in the UK. The Journal of Agricultural Science, Cambridge 136: 15–33.
van Herwaarden, A.F., Richards, R.A., Farquhar, G.D. and Angus, J.F. 1998a. Haying-off, the negative grain yield response to nitrogen fertiliser. I. Biomass, grain yield and water use. Australian Journal of Agricultural Research 49: 1067–1081.
van Herwaarden, A.F., Angus, A.F., Richards, R.A. and Farquhar G.D. 1998b. Haying-off, the negative grain yield response to nitrogen fertiliser. II. Carbohydrate and protein dynamics. Australian Journal of Agricultural Research 49: 1083–1093.
Werker, A.R. and Gilligan, C.A. 1990. Analysis of the effects of selected agronomic factors on the dynamics of the take-all disease of wheat in field plots. Plant Pathology 39: 161–177.
Whingwiri, E.E. and Kemp, D.R. 1980. Spikelet development and grain yield of the wheat ear in response to applied nitrogen. Australian Journal of Agricultural Research 31: 637–647.
White, R.E., Wellings, S.R. and Bell, J.P. 1983. Seasonal variations in nitrate leaching in structured clay soils under mixed land use. Agricultural Water Management 7: 391–410.
Zadoks, J.C., Chang, T.T. and Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Research 44: 415–421.
Zebarth, B.J. and Rosen, C.J. 2007. Research perspective on nitrogen BMP development for potato. American Journal of Potato Research 84: 3–18.
GS, growth stage.
GS, growth stage.
GPE, grains per ear;
TGW, thousand grain weight;
REN, recovery efficiency of N;
AEN, agronomic efficiency of N;
PEN, physiological efficiency of N.
r is the Pearson correlation coefficient between the component of yield for the respective year and grain yield for the N application timing treatments (excluding 0N).
P indicates the significance of the correlation.
RE data are the mean of 2011 and 2012.