Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

  • 1 Department of Molecular Biology and Biotechnology, Damascus

Summary

Enhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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  • Adam, A., Arabi, M.I.E., Idris, I. and Al-Shehadah, E. 2017. Effect of several rhizobacteria strains on barley resistance against Pyrenophora graminea under field conditions. Hellenic Plant Protection Journal, 10: 35-45.

  • Adam, A., Idris, I. and Ayyoubi, Z. 2013. In vitro Pseudomonas putida BTP1-induced systemic resistance in grapevine rootstocks against phylloxera (Daktulosphaira vitifoliae) Advances in Horticultural Science, 27(4): 137-142.

  • Adam, A., Idris, I., Khalil, N. and Houssian, K. 2016. Induced resistance in potato plants by a non-pathogenic Pseudomonas putida BTP1 against potato tuber moth (Phthorimaea operculella Zeller). Advances in Horticultural Science, 30(1): 47-52.

  • Adam, A., Makee, H. and Idris, I. 2012. The influence of a non-pathogenic Pseudomonas putida strain BTP1 on reproduction and development of grape phylloxera. Advances in Horticultural Science, 26(2): 75-80.

  • Adam, A., Ongena, M., Duby, F., Dommes, J. and Thonart, P. 2008. Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putida strain BTP1. BMC Plant Biology, 8: 113.

  • Adrees, H., Haider, M.S., Anjum, T. and Akram, W. 2019. Inducing systemic resistance in cotton plants against charcoal root rot pathogen using indigenous rhizospheric bacterial strains and chemical elicitors. Crop Protection, 115: 75-83.

  • Ahmed, B., Zaidi, A., Khan, M.S., Rizvi, A., Saif, S. and Shahid, M. 2017. Perspectives of Plant Growth Promoting Rhizobacteria in Growth Enhancement and Sustainable Production of Tomato. In. Zaidi, A. and Khan, M.S. (eds), Microbial Strategies for Vegetable Production. pp.125-149.

  • Anonymous 1988. STAT-ITCF, Programme, MICROSTA, realized by ECOSOFT 2nd Ver. Institut Technique des Cereals et des Fourrages Paris, France. Arabi, M.I.E. 2005. Inheritance of partial resistance to spot blotch in barley. Plant Breeding, 124(6): 605-607.

  • Arabi, M.I.E. and Jawhar, M. 2003. Pathotypes of Cochliobolus sativus (spot blotch) on barley in Syria. Journal of Plant Pathology, 85: 193-196.

  • Ataoglu, N., Turan, M. and Sezen, Y. 2004. Effects of phosphorus solubilizing bacteria (Bacillus megatherium) and growing media on growing performance and mineral contents of corn plant (Zea mays L.). In ‘Proceedings of the International Soil Congress on Natural Resource Management for Sustainable Development’, Erzurum, Turkey, pp. 10-18.

  • Bakker, P.A.H.M., Pieterse, C.M.J. and van Loon, L.C. 2007. Induced Systemic Resistance by Fluorescent Pseudomonas spp. Phytopathology, 97(2): 239-243.

  • Cakmakci, R., Kantar, F. and Sahin, F. 2001. Effect of N2-fixing bacterial inoculations on yield of sugar beet and barley. Journal of Plant Nutrition and Soil Science, 164: 527–531.

  • Choudhary, D.K. and Johri, B.N. 2009. Interaction of Bacillus spp. and plant-with special reference to induced systemic resistance (ISR). Microbiological Research, 164(5): 493-513.

  • Córdova-Campos, O., Adame-Álvarez, R.M., Acosta-Gallegos, J.A. and Heil, M. 2012. Domestication affected the basal and induced disease resistance in common bean (Phaseolus vulgaris). European Journal of Plant Pathology, 134: 367-379.

  • Çavuşoğlu, K. and Kabar, K. 2008. Bazı bitki büyüme düzenleyicilerinin tuzlu koşullar altındaki arpa tohumlarının çimlenmesi üzerindeki etkilerinin karşılaştırılması. Fırat University Journal of Engineering Science, 20(1): 43-55.

  • Dann, E., Diers, B., Byrum, J. and Hammerschmidt, R. 1998. Effect of treating soybean with 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH) on seed yields and the level of disease caused by Sclerotinia sclerotiorum in field and greenhouse studies. European Journal of Plant Pathology, 104: 271-278.

  • De Silva, A., Petterson, K., Rothrock, C. and Moore, J. 2000. Growth promotion of highbush blueberry by fungal and bacterial inoculants. HortScience, 35: 1228–1230.

  • De Vleesschauwer, D. and Höfte, M. 2009. Rhizobacteria-induced systemic resistance. Advances in Botanical Research, 51: 223-281.

  • Durrant, W.E. and Dong, X. 2004. Systemic acquired resistance. Annual Review of Phytopathology, 42: 185-209.

  • Ghazvini, H. and Tekauz, A. 2008. Host pathogen interactions among barley genotypes and Bipolar-is sorokiniana isolates. Plant Disease, 92: 225-233.

  • Gutiérrez-Maňero, F.J., Ramos-Solano, B., Probanza, A.n., Mehouachi, J., Tadeo, F.R. and Talon, M. 2001. The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins. Physiologia Plantarum, 111: 206-211.

  • Haas, D. and Défago, G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Reviews Microbiology, 3: 307-319.

  • Jacques, P., Hbid, C., Destain, J., Razafindralambo, H., Paquot, M., Pauw, E.D. and Thonart, P. 1999. Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design. Applied Biochemistry and Bio-technology, 77-79: 223-233.

  • Joo, G.-J., Kim, Y.-M., Kim, J.-T., Rhee, I.-K., Kim, J.-H. and Lee, I.-J. 2005. Gibberellins-Producing Rhizobacteria Increase Endogenous Gibberellins Content and Promote Growth of Red Peppers. The Journal of Microbiology, 43(6): 510-515.

  • King, E.O., Ward, M.K. and Raney, D.E. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine, 44: 301-307.

  • Kloepper, J.W., Ryu, C.M. and Zhang, S.A. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology, 94(11): 1259-1266.

  • Majeed, A., Abbasi, M.K., Hameed, S., Imran, A. and Rahim, N. 2015. Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion. Frontiers in Microbiology, 6: 198.

  • Mathre, D.E. 1990. Compendium of Barley Disease’ 2nd edn. (American Phytopathological Society Press: St. Paul, MN).

  • Meldrum, S., Ogle, H. and Platz, G. 2000. Pathotypes of Bipolaris sorokiniana on barley in Australia. In ‘Proceedings of the 9th Australian barley technical symposium’, Melbourne, Australia. Available at http://www.ncbi.nlm.gov.

  • Ongena, M., Daayf, F., Jacques, P., Thonart, P., Benhamou, N., Paulitz, T.C., Cornelis, P., Koedam, N. and Bélanger, R.R. 1999. Protection of cucumber against Pythium root rot by fluorescent Pseudomonads: predominant role of induced resistance over siderophores and antibiosis. Plant Pathol., 48(1): 66-76.

  • Ongena, M., Duby, F., Rossignol, F., Fauconnier, M.L., Dommes, J. and Thonart, P. 2004. Stimulation of the lipoxygenase pathway is associated with systemic resistance induced in bean by a nonpathogenic Pseudomonas strain. Molecular Plant-Microbe Interactions, 17(9): 1009-1018.

  • Ongena, M., Jacques, P., Delfosse, P. and Thonart, P. 2002. Unusual traits of the pyoverdin-mediated iron acquisition system in Pseudomonas putida strain BTP1. Biometals, 15: 1-13.

  • Ongena, M., Jourdan, E., Adam, A., Schafer, M., Budzikiewicz, H. and Thonart, P. 2008. Amino Acids, Iron, and Growth Rate as Key Factors Influencing Production of the Pseudomonas Putida BTP1 Benzylamine Derivative Involved in Systemic Resistance Induction in Different Plants. Microbial Ecology, 55: 280-292.

  • Orhan, E., Esitken, A., Ercisli, S., Turan, M. and Sahin, F. 2006. Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci. Hortic. 111(1): 38-43.

  • Pieterse, C.M.J., Van Wees, S.C.M., Ton, J., Van Pelt, J.A. and Van Loon, L.C. 2002. Signaling in rhizobacteria-induced systemic resistance in Arabidopsis thaliana. Plant Biol., 4(5): 535-544.

  • Pinedra, A., Zheng, S., van Loon, J., Pieterse, C. and Dicke, M. 2010. Helping plants to deal with insects: the rol of benefical soil-borne microbes. Trends in Plant Science, 15: 507-514.

  • Raaijmakers, J.M., Leeman, M., Van Oorschot, M.M.P., van der Sluis, I., Schippers, B. and Bakker, P.A.H.M. 1995. Dose-response relationships in biological control of Fusarium wilt of radish by Pseudomonas spp. Phytopathology, 85(10): 1075-1081.

  • Ramamoorthy, V., Viswanathan, R., Raguchander, T., Prakasam, V. and Samiyappan, R. 2001. Induction of systemic resistance by plant growth promoting rhizobacteria in crop plants against pests and diseases. Crop Protection, 20(1): 1-11.

  • Reglinski, T.a.W., D. 2009. Induced resistance for plant disease control in disease control in crops. Oxfored UK. Wiley- Blackwell. pp.

  • Resende, M.L.V., Nojosa, G.B.A., Cavalcanti, L.S., Aguilar, M.A.G., Silva, L.H.C.P., Perez, J.O., Andrade, G.C.G., Carvalho, G.A. and Castro, R.M. 2002. Induction of resistance in cocoa against Crinipellis perniciosa and Verticillium dahlia by acibenzolar-S-methyl (ASM). Plant Pathology, 51: 621-628.

  • Rizvi, A., Zaidi, A., Khan, M.S., Saif, S., Ahmed, B. and Shahid, M. 2017. Growth Improvement and Management of Vegetable Diseases by Plant Growth-Promoting Rhizobacteria. In. Zaidi, A. and Khan, M.S. (eds), Microbial Strategies for Vegetable Production. pp.99-123. Saravanakumar, D., Vijayakumar, C., Kumar, N. and Samiyappan, R. 2007. PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protection, 26(4): 556-565.

  • Tucci, M., Ruocco, M., De Masi, L., De Palma, M. and Lorito, M. 2011. The beneficial effect of Trichoderma spp. on tomato is modulated by plant geno-type. Molecular Plant Pathology, 12: 341-354.

  • Turan, M., Ataoglu, N. and Sezen, Y. 2004. Effects of phosphorus solubilizing bacteria (Bacillus megaterium) on yield and phosphorus contents of tomato plant (Lycopersicon esculentum L.) III. In ‘Proceedings of the National Fertilizer Congress. Farming-Industry-Environment’, Tokat, Turkey, 11–13 October.

  • van Leur, J., Alamdar, M. and S., K. 1997. Effect of Cochliobolus sativus on yields of barley under experimental conditions in northern Syria. Australian Journal of Agricultural Research, 48: 1-7.

  • Van Loon, L.C. 2007. Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology, 119: 243-254.

  • Van Loon, L.C., Bakker, P.A.H.M. and Pieterse, C.M.J. 1998. Systemic resistance induced by rhizo-sphere bacteria. Annual Review of Phytopathology, 36: 453-483.

  • Van Wees, S.C.M., van der Ent, S. and Pieterse, C. 2008. Plant immun responses triggered by benefical microbes. Current Opinion in Plant Biology, 11: 443-448.

  • Vlot, A.C., Klesig, D.F. and Park, S.W. 2008. Systemic acquired resistance: the elusive signal(s). Current Opinion in Plant Biology, 11: 436-442.

  • Walters, D.R., Havis, N.D., Paterson, L., Taylor, J. and Walsh, D.J. 2011a. Cultivar effects on the expression of induced resistance in spring barley. Plant Disease, 95: 595-600.

  • Yasmin, S., Zaka, A., Imran, A., Zahid, M.A., Yousaf, S., Rasul, G., Arif, M. and Mirza, M.S. 2016. Plant Growth Promotion and Suppression of Bacterial Leaf Blight in Rice by Inoculated Bacteria. PLOS ONE, 11(8): 1-19.

  • Zadoks, J.C., Chang, T.T. and Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Research, 14: 415−421.

  • Zehnder, G., Kloepper, J., Yao, C. and Wei, G. 1997. Induction of systemic resistance in cucumber against cucumber beetles (Coleoptera: Chrysomelidae) by plant growth-promoting rhizobacteria Journal of Economic Entomology, 90(2): 391-396.

  • Zehnder, G.W., Murphy, J.F., Sikora, E.J. and Kloepper, J.W. 2001. Application of rhizobacteria for induced resistance. European Journal of Plant Pathology, 107(1): 39-50.

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