The mite fauna in foliage and litter of a sprayed alfalfa hay field with the acaricide-insecticide bifenthrin, was studied based on monthly samplings from foliage and litter in Central Greece between 2008–2009. Potential differentiations between this field and two adjacent alfalfa hay fields, which were not subjected to pesticide applications and were managed with different number of cuttings, were also evaluated in terms of population fluctuation over time, population density, species richness, diversity and spatial distribution. The sprayed field hosted 50 and 68 species and morphospecies in foliage and litter respectively, depicting high relative abundance of oribatid and prostigmatic mites.