Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees
, and | Jan 24, 2018
Published Online: Jan 24, 2018
Page range: 40 - 46
Received: Dec 22, 2017
Accepted: Jan 09, 2018
DOI: https://doi.org/10.2478/hppj-2018-0005
Keywords
© 2018 K.M. Kasiotis et al., published by De Gruyter Open
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g bee body weight (bw) for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.