Semiquantitative Assessment of Dishevelled-3 Phosphorylation Status by Mass Spectrometry

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Abstract

The focus of this paper is the human Dishevelled 3 protein (hDvl3), an essential component of the Wnt signalling pathway that contributes to their regulation. Mass spectrometry-based analysis of hDvl3 phosphorylations induced by eight associated kinases was performed revealing several dozens of phosphorylation sites. The main outcome of this study was the description of Dvl phosphorylation “patterns” induced by individual kinases

[1] Kersten, B., Agrawal, G. K., Iwahashi, H., Rakwal, R.: Plant phosphoproteomics: A long road ahead, Proteomics, 2006 6(20), 5517-5528 DOI: 10.1002/pmic.200600232

[2] Bernatík, O., Šedová, K., Schille, C., Ganji, S. R., Cˇ ervenka, I., Trantírek, L., Schambony, A., Zdráhal, Z., Bryja, V.: Functional analysis of Dishevelled- 3 phosphorylation identifies distinct mechanisms driven by casein kinase 1 epsilon and frizzled5, J. Biol. Chem., 2014 34(289), 23520-23533 DOI: 10.1074/jbc.M114.590638

[3] Yanfeng, W. A., Berhane, H., Mola, M., Singh, J., Jenny, A., Mlodzik, M.: Functional dissection of phosphorylation of Disheveled in Drosophila, Dev. Biol., 2011 360, 132-142 DOI: 10.1016/j.ydbio.2011.09.017

[4] Klimowski, L. K., Garcia, B. A., Shabanowitz, J., Hunt, D. F., Virshup, D. M.: Site-specific casein kinase 1ε-dependent phosphorylation of Dishevelled modulates β-catenin signaling, FEBS J., 2006 273, 4594-4602 DOI: 10.1111/j.1742 4658.2006.05462.x.

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