Antibacterial activity of ethanolic extracts of some moss species

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Summary

Introduction: For centuries, mosses have been used in traditional medicine due to their antibacterial, antifungal, and antiviral activities. Objective: The present study was designed to evaluate the antibacterial activity of ethanolic extracts obtained from 12 moss species: Brachythecium albicans, Bryum argenteum, Ceratodon purpureus, Dicranum scoparium, Dryptodon pulvinatus, Orthotrichum anomalum, Oxyrrhynchium hians, Plagiomnium undulatum, Polytrichum juniperinum, P. piliferum, Schistidium crassipilum, and Syntrichia ruralis. Methods: The antimicrobial activity of extracts was investigated against three Gram(+) bacteria (Enterococcus faecalis, Staphylococcus aureus, and Streptococcus pyogenes) and two Gram(-) bacteria (Escherichia coli and Klebsiella pneumoniae), using the agar disc-diffusion method. Results: The high activity against all investigated bacteria was determined for extracts of D. pulvinatus, P. undulatum, B. argenteum, S. crassipilum, O. anomalum (mean inhibition zone: 11.3-13.1 mm) and to a lesser extent in the case of D. scoparium (8.3 mm). Extracts from P. juniperinum and P. piliferum showed activity only against Gram-positive bacteria, with an inhibition zone from 7.3 to 9.7 mm. Four species: B. albicans, C. purpureus, O. hians, and S. ruralis had not antibacterial properties. Conclusions: The obtained results indicate that mosses could be a significant source of antibacterial agents. For the first time, we presented antibacterial activity of ethanolic extracts from S. crassipilum and O. anomalum.

1. The Plant List, The Bryophytes (Mosses and liverworts). http://www.theplantlist.org/1.1/browse/B/, 2013 (accessed 25.07.2017).

2. Ochyra R, Żarnowiec J, Bednarek-Ochyra H. Census catalogue of Polish mosses. Polish Academy of Sciences, Institute of Botany, Kraków 2003:1-372.

3. Szweykowski J. An annotated checklist of Polish liverworts and hornworts. Polish Academy of Sciences, Institute of Botany, Kraków 2006:1-114.

4. Ignatov MS, Ignatova EA. Moss flora of the Middle European Russia. Vol. 1: Sphagnaceae-Hedwigiaceae. KMK Scientific Press, Moscow. Arctoa 2003; 11(suppl. 1):1-608.

5. Ignatov MS, Ignatova EA. Moss flora of the Middle European Russia. Vol. 2: Fontinalaceae- Amblystegiaceae. KMK Scientific Press, Moscow. Arctoa 2003; 11(suppl. 2):609-944.

6. Atherton IDM, Bosanquet SDS, Lawley M. Mosses and liverworts of Britain and Ireland: a field guide. British Bryological Society, 2010:1-848.

7. Ando H, Matsuo A. Applied bryology. In: Cramer J, ed. Advances in Bryology. Vaduz, West Germany: Schultze-Motel W, 1984:2-133.

8. Head KA. Natural approaches to prevention and treatment of infections of the lower urinary tract. Altern Med Rev 2008; 13(3):227-244.

9. Kurlenda J, Grinholc M. Alternative therapies in Staphylococcus aureus diseases. Acta Biochim Pol 2012; 59(2):171-184.

10. Glime JM. Economic and ethnic uses of bryophytes. Flora of North America 2007; 27:14-41.

11. Staniaszek-Kik M, Stefańska-Krzaczek E. Mszaki - małe gabaryty (nie)duży pożytek? Studia Materiały CEPL w Rogowie 2014; 16(38/1):129-135.

12. Sobolewska A, Sztanke M, Pasternak K. Składniki borowiny i jej właściwości lecznicze. Balneol Pol 2007; 2:93-98.

13. Asakawa Y. Biologically active substances from bryophytes. In: Chopra RN, Bhatla SC, eds. Bryophyte development - physiology and biochemistry. Boston, CRC Press, 1990:259-287.

14. Nikolajeva V, Liepina L, Petrina Z, Krumina G, Grube M, Muiznieks I. Antibacterial activity of extracts from some bryophytes. Adv Microbiol 2012; 2:345-353. doi:

15. Ertürk Ö, Sahin H, Ertürk EY, Hotaman HE, Koz B, Özdemir Ö. The antimicrobial and antioxidant activities of extracts obtained from some moss species in Turkey. Herba Pol 2015; 61(4):52-65. doi:

16. Singh M, Rawat AKS, Govindarajan R. Antimicrobial activity of some Indian mosses. Fitoterapia 2007; 78:156-158. doi:

17. Veljić M, Đurić A, Soković M, Ćirić A, Glamočlija J, Marin PD. Antimicrobial activity of methanol extracts of Fontinalis antipyretica, Hypnum cupressiforme, and Ctenidium molluscum. Arch Biol Sci Belgrade 2009; 61(2):225-229. doi:

18. Asakawa Y, Ludwiczuk A, Nagashima F. Phytochemical and biological studies of bryophytes. Phytochemistry 2013; 91:52-80. doi:

19. Dey A, Mukherjee A. Therapeutic potential of bryophytes and derived compounds against cancer. J Acute Dis 2015; 4(3):236-248. doi:

20. Basile A, Giordono S, Lopez-Saez JA, Cobianchi RC. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry 1999; 52:1479-1482. doi:

21. Saritas Y, Sonwa MM, Iznaguen H, Konig WA, Muhle H, Mues R. Volatile constituents in mosses (Musci). Phytochemistry 2001; 57:443-457. doi:

22. Elibol B, Ezer T, Kara R, Çelik GY, Çolak E. Antifungal and antibacterial effects of some acrocarpic mosses. Afr J Biotechnol 2011; 10(6):986-989.

23. More G, Tshikalange TE, Lall N, Botha F, Meyer JJM. Antimicrobial activity of medicinal plants against oral microorganisms. J Ethnopharmacol 2008; 119(3):473-477. doi:

24. CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, 11th ed., CLSI document M02-A11, vol. 32, no 1. Clinical and Laboratory Standards Institute, USA, 2012.

25. McFarland J. The nephelometer: an instrument for estimating the number of bacteria in suspensions for calculating the opsonic index and vaccines. J Am Med Assoc 1907; 49:1176.

26. Sabovljevic A, Sokovic M, Sabovljevic M, Grubisic D. Antimicrobial activity of Bryum argenteum. Fitoterapia 2006; 77:144-145. doi:

27. Krishnan R, Manoj GS, Murugan K. In vitro microbicidal potentiality of Targionia hypophylla L. and Bryum species - bryophytes. Int J Pharm Pharmac Sci 2012; 4(2):410-413.

28. Bodade RG, Borkar PS, Arfeen S, Khobragade CN. In-vitro screening of bryophytes for antimicrobial activity. J Med Plants 2008; 7(4):23-28.

29. Dulger B, Yayintas OT, Gonuz A. Antimicrobial activity of some mosses from Turkey. Fitoterapia 2005; 76:730-732.

30. Veljić M, Tarbuk M, Marin PD, Ćirić A, Soković M, Marin M. Antimicrobial activity of methanol extracts of some genuine mosses from Serbia. Pharmac Biol 2008; 46(12):871-875. doi:

31. Savaroglu F, Ilhan S, Filik-Iscen C. An evaluation f the antimicrobial activity of some Turkish mosses. J Med Plants Res 2011; 5(14):3286-3292.

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