Accumulation of phenolic compounds in the purple betony herb (Stachys officinalis L.) originated from cultivation

Open access

Summary

Introduction: Purple betony (Stachys officinalis L., Lamiaceae) is a perennial of versatile medicinal usage. Nowadays, in Poland betony herb is collected exclusively from wild growing plants. Decreasing number of its natural sites results in lack of the herb supply and thus, in its limited usage.

Objective: The aim of the study was to determine the effects of the age of plant and term of raw material harvest on its yield and quality in cultivation conditions.

Methods: The observations were carried out on 2- and 3-year-old plants. During vegetation the herb was collected for four times. The raw material was subjected to chemical analysis. Tannins (as pyrogallol equivalent) were determined according to Polish Pharmacopoeia, phenolic acids and flavonoids – by HPLC.

Results: The mass of herb, both in the second and third year, had increased from the beginning of vegetation up to seed setting. The highest content of tannins was found in the herb collected at the vegetative stage of plant development (2.05% in the second and 2.91% in the third year). Four phenolic acids (chlorogenic, ferulic, caffeic and rosmarinic acids) and five flavonoid compounds (orientin, luteolin-7-glucoside, apigenin-7-glucoside, apigenin-3-glucoside, apigenin) were identified in the obtained raw materials. In these groups, the dominant compounds were caffeic acid and apigenin. The highest content of caffeic acid was found at the beginning of plant vegetation, whereas apigenin – at the stage of full blooming and seed setting.

Conclusion: In cultivation conditions, purple betony produces high mass of herb which may be used as a valuable raw material in herbal industry.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Kobzar AYa Nikov GK. Flavonoids of the epigal part of Betonica officinalis. Khim Prir Soedin 1987; 5:636-637.

  • 2. Dušek K Dušková E Smékalová K. Variability of morphological characteristic and content of active substances in Betonica officinalis L. in the Czech Republic. Agriculture (Pol’nohospodarstvo) 2009; 2:102-110.

  • 3. Kostrakiewicz-Gierałt K. The impact of different habitat conditions on the variability of wild populations of a medicinal plant Betonica officinalis L. Ecol Balk 2015; 7(1):51-61.

  • 4. Dušek K Dušková E Smékalová K. Betonica officinalis L. in the Czech Republic. I. Variability of morphological characteristic. Herba Pol 2010; 56(2):7-20.

  • 5. Imbrea I Butnariu M Nicolin A Imbrea F Prodan M. Valorising the species Stachys officinalis (L.) Trevis. from South-Western Romania. Res J Agric Sci 2011; 43(2):198-203.

  • 6. Vundać VB Pfeifhofer HW Brantner AH Males Z Plazibat M. Essential oils of seven Stachys taxa from Croatia. Biochem Syst and Ecol 2006; 34:875-881. doi: http://dx.doi.org/10.1016/j.bse.2006.04.010

  • 7. Marin PD Grayer RJ Grujic-Jovanovic S Kite GC Veitch NC. Glycosides of tricetin methyl ethers as chemosystematic markers in Stachys subgenus Betonica. Phytochem 2004; 65:1247-1253. doi: http://dx.doi.org/10.1016/j.phytochem.2004.04.014

  • 8. Strzelecka H Kowalski J. Encyklopedia zielarstwa i ziołolecznictwa. Wydawnictwo Naukowe PWN Warszawa 2000:74.

  • 9. Adams M Berset C Kessler M Hamburger M. Medicinal herbs for the treatment of rheumatic disorders – A survey of European herbals from the 16th and 17th century. J Ethnopharmacol 2009; 121:343-359. doi: http://dx.doi.org/10.1016/j.jep.2008.11.010

  • 10. Vundać VB Brantner AH Plazibat M. Content of polyphenolic constituents and antioxidant activity of some Stachys taxa. Food Chem 2007; 104:1277-1281. doi: http://dx.doi.org/10.1016/j.foodchem.2007.01.036

  • 11. Leporatti ML Ivancheva S. Preliminary comparative analysis of medicinal plants used in traditional medicine of Bulgaria and Italy. J Ethnopharmacol 2003; 87:123-142. doi: http://dx.doi.org/10.1016/S0378-8741(03)00047-3

  • 12. Jäger AK Gauguin B Andersen A Gudiksen L. Screening of plants used in Danish folk medicine to treat epilepsy and convulsions. J Ethnopharmacol 2006; 105:294-300. doi: http://dx.doi.org/10.1016/j.jep.2005.10.015

  • 13. Dušek K Dušková E Smékalová K. Betonica officinalis L. in the Czech Republic. II. Seed production and quality and variability of total polyphenols content. Herba Pol 2010; 56(3):15-23.

  • 14. Grochowski W Grochowski A. Leśne grzyby owoce zioła. Zbiór i wykorzystanie. Warszawa. Państwowe Wydawnictwo Rolnicze i Leśne 1994:174-175.

  • 15. Buchwald W Dedio I. Evaluation of raw material of Betonica officinalis L. from cultivation. Herba Pol 1998; 44(4):403-408.

  • 16. Buchwald W Czapska A. Tannins and phenolic acids in herb of domestic species of Betonica L. and Stachys L. genera. Herba Pol 1995; 41(4):198-202.

  • 17. Polish Pharmacopoeia VIII. Warszawa. Polskie Towarzystwo Farmaceutyczne 2008.

  • 18. Howke RG. A comparative study of cultivated Stachys. Plant Evaluation Notes 2005; Chicago Botanic Garden RE5875:1-4.

  • 19. Hajdari A Mustafa B Franz CH Novak J. Total flavonoids total phenolics and antioxidant activity of Betonica officinalis L. from Kosovo. Acta Hort 2010; 860:75-80. doi: http://dx.doi.org/10.17660/ActaHortic.2010.860.8

  • 20. Kobzar AYa. Phytochemical study of Betonica officinalis. I. Isolation of biologically active substances from the epigeal part of the plant. Khim Prir Soedin 1986; 2:223-224.

  • 21. Kobzar AYa. Phytochemical study of Betonica officinalis. II Acids from the epigeal part of Betonica. Khim Prir Soedin 1986; 2:239-240.

  • 22. Vundać VB Maleš Ż Plazibat M Golja P Cetina-Čižmek B. HPTLC determination of flavonoids and phenolic acids in some Croatian Stachys taxa. J Planar Chromat 2005; 18:269-273. doi: http://dx.doi.org/10.1556/JPC.18.2005.4.3

Search
Journal information
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 274 136 2
PDF Downloads 138 88 0