Determination of chlorogenic and gallic acids by UPLC-MS/MS
, Bogna Opala
, Zdzisław Łowicki
, Anna Krajewska-Patan
, Waldemar Buchwald
, Bogusław Czerny
, Sebastian Mielcarek
, Dariusz Boroń
, Anna Bogacz
, and Przemysław M. Mrozikiewicz
1 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland
2 Department of Pharmacology and Experimental Biology Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland
3 Team of Botany and Agriculture of Medicinal Plants Department of Botany, Breeding and Agricultural Technology Institute of Natural Fibres and Medicinal Plants Kolejowa 2 62-064 Plewiska/Poznań, Poland
4 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland / Department of General Pharmacology and Pharmacoeconomics Pomeranian Medical University Żołnierska 48 70-204 Szczecin, Poland
5 Departament of Histology and Embriology Medical University of Silesia Jordana 19 41-808 Zabrze, Poland
6 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland / Laboratory of Experimental Pharmacogenetics Department of Clinical Pharmacy and Biopharmacy Poznań University of Medical Sciences Św. Marii Magdaleny 14 61-861 Poznań, Poland
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.
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