Novel method of detection of phenylpropanoids of Rhodiola roots species

Agnieszka Gryszczyńska 1 , Bogna Opala 1 , Anna Krajewska-Patan 2 , Zdzisław Łowicki 1 , Waldemar Buchwald 3 , Sebastian Mielcarek 1 , Anna Bogacz 4 , Monika Karasiewicz 1 , Dariusz Boroń 5 , Bogusław Czerny 6 ,  and Przemysław M. Mrozikiewicz 4
  • 1 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland
  • 2 Department of Pharmacology and Experimental Biology Institute of Natural Fibres and Medicinal PlantsLibelta 27 61-707 Poznań, Poland
  • 3 Team of Botany and Technology of Medicinal Plants Department of Botany, Breeding and Agricultural Technology Institute of Natural Fibres and Medicinal Plants Kolejowa 2 62-064 Plewiska/Poznań, Poland
  • 4 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland / Laboratory of Experimental Pharmacogenetics Department of Clinical Pharmacy and Biopharmacy Poznań University of Medical Sciences Św. Marii Magdaleny 14 61-861 Poznań, Poland
  • 5 Departament of Histology and Embriology Medical University of Silesia Jordana 19 41-808 Zabrze, Poland
  • 6 Department of Quality Control of Medicinal Products and Dietary Supplements Institute of Natural Fibres and Medicinal Plants Libelta 27 61-707 Poznań, Poland / Department of General Pharmacology and Pharmacoeconomics Pomeranian Medical University Żołnierska 48 70-204 Szczecin, Poland

Summary

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

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