Morphological and molecular genetic analysis of Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) (Nematoda, Acuariidae) from the barn owl (Tyto alba) and the common kestrel (Falco tinnunculus) in Austria

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In the framework of the biodiversity initiative and barcoding project “Austrian Barcode of Life” (ABOL) post mortem examinations of the gastro-intestinal tracts of different species of wild birds were carried out and several adult helminths were retrieved. In the gizzard of two barn owls (Tyto alba) and one common kestrel (Falco tinnuculus) acuariid nematodes belonging to the species Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) were discovered. This report illustrates the identification of this parasitic nematode by morphometric comparison and scanning electron microscopic photographs. Furthermore, genetic identification of individual parasites based on a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the nuclear 18S ribosomal RNA gene was carried out. This report constitutes the first COI-based DNA barcoding of S. (S.) laticeps and its first record in the barn owl (Tyto alba) in Austria.

Acosta, I., Hernández, S., Gutiérrez, P.N., Martínez-Cruz, M.S., Hernández, E., Buffoni, L., Martínez-Moreno, F.J. (2010): Acuaroid nematodes in the common kestrel (Falco tinnunculus) in the south of Spain. Vet. J., 183: 234 – 237. DOI: 10.1016/j.tvjl.2008.10.013

Aşti, C., Cengiz, G., Sarimehmetoğlu, H.G (2017): A case of Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) Railliet, Henry and Sisoff, 1912 (Nematoda, Acuariidae) in common kestrel (Falco tinnunculus). Ankara Üniv. Vet. Fak. Derg., 64: 65 – 68. DOI: 10.1501/Vetfak_0000002776

Anderson, R.C. (2000): Nematode Parasites of Vertebrates, their Development and Transmission. 2nd Edition, Walingford, UK, CABI Publishing, 650 pp.

Borgsteede, F.H.M., Okulewicz, A., Zoun, P.E.F., Okulewicz, J. (2003): The helminth fauna of birds of prey (Accipitriformes, Falconiformes and Strigiformes) in the Netherlands. Acta Parasitol., 48(3): 200 – 207

Chabaud, A.G. (1975): Keys to the genera of the Order Spirurida. Part 2. Spiruroidea, Habronematoidea and Acuarioidea. In: Anderson, R.C., Chabaud, A.G., Willmott, S. (Eds) CIH Keys to the Nematode Parasites of Vertebrates. Farnham Royal, UK: Commonwealth Agricultural Bureaux, pp. 29 – 58

Cram, E.B. (1927): Bird parasites of the nematode suborders Strongylata, Ascaridata, and Spirurata. Bull. U. S. Natl. Mus., 140: 1 – 465

Cramp, S., Simmons, K.E.L. (Eds) (1980): The Birds of the Western Palearctic. Volume II. Oxford, New York, USA, Oxford University Press, 695 pp.

Etchegoin, J.A., Cremonte, F., Navone, G.t. (2000): Synhimantus (Synhimantus) laticeps (Rudolphi, 1819) Railliet, Henry et Sisoff, 1912 (Nematoda, Acuariidae) parasitic in Tyto alba (Gmelin) (Aves, Tytonidae) in Argentina. Acta Parasitol., 45(2): 99 – 106

Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoeck, R. (1994): DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Marine Biol. Biotechnol., 3(5): 294 – 299.

Furmaga, S. (1957): The helminth fauna of predatory birds (Accipitres et Striges) of the environment of Lublin. Acta Parasitol., 5(13): 215 – 297

Honisch, M. (2008): Phylogenie von Nematoden einheimischer Greifvögel [Phylogeny of nematodes from birds of prey]. Doctoral Thesis, Germany, Berlin: Freie Universität Berlin, Fachbereich Veterinärmedizin (In German)

Honisch, M., Krone, O. (2008): Phylogenetic relationships of Spiruromorpha from birds of prey based on 18S rDNA. Journal of Helminthology, 82: 129 – 133. DOI: 10.1017/S0022149X08912359

Kotremba, J. (1978): Beitrag zur Helminthen- und Acanthocephalenfauna heimischer Greifvögel [Helminths and acanthocephala of endemic birds of prey]. Doctoral Thesis, Austria, Vienna: University of Veterinary Medicine, Vienna (In German)

Krone, O. (2000): Endoparasites in free-ranging birds of prey in Germany. In: Lumeij, J.T., Remple, J.D., Redig, P.T., Lierz, M., Cooper, J.E. (Eds) Raptor Biomedicine III. Lake Worth, Florida, USA: Zoological Education Network, Inc., pp. 101 – 116

Kutzer, E., Frey, H., Kotremba, J. (1980): Zur Parasitenfauna österreichischer Greifvögel (Falconiformes) [Parasites of Austrian birds of prey (Falconiformes)]. Angew. Parasitol., 21: 183 – 205 (In German)

Kutzer, E., Frey, H., Nöbauer, H. (1982): Zur Parasitenfauna österreichischer Eulenvögel (Strigiformes) [Parasite fauna of Austrian owls (Strigiformes)]. Angew. Parasitol., 23: 190 – 197 (In German)

Osche, G. (1955): Bau, Entwicklung und systematische Bedeutung der Cordons der Acuariidae (Nematoda) am Beispiel von Stammerinema soricis (Tiner 1951) gen. nov. [Structure, development and systematic significance of cordons of Acuariidae (Nematode), an example of Stammerinema soricis (Tiner 1951) gen. nov.]. Z. Parasitenkd., 17(2): 73 – 92 (In German)

Perera, A., Maia, J.P.M.C, Jorge, F., Harris, D.J. (2013): Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences. J. Helminthol., 87(2): 189 – 194. DOI: 10.1017/S0022149X12000181

Rudolphi, C.A. (1819): Entozoorum synopsis cui accedunt mantissa duplex et indices locupletissimi. Berlin, Germany, Sumptibus Augusti Rücker, 811 pp.

Sanmartín, M.L., Álvarez, F., Barreiro, G., Leiro, J. (2004): Helminth fauna of falconiform and strigiform birds of prey in Galicia, Northwest Spain. Parasitol. Res., 92: 255 – 263. DOI: 10.1007/s00436-003-1042-z

Santoro, M., Tripepi, M., Kinsella, J.M., Panebianco, A., Mattiucci, S. (2010): Helminth infestation in birds of prey (Accipitriformes and Falconiformes) in Southern Italy. Vet. J., 186: 119 – 122. DOI: 10.1016/j.tvjl.2009.07.001

Santoro, M., Mattiucci, S., Nascetti, G., Kinsella, J.M., Di Prisco, F., Troisi, S., D'Alessio, N., Veneziano, V., Aznar, F.J. (2012): Helminth communities of owls (Strigiformes) indicate strong biological and ecological differences from birds of prey (Accipitriformes and Falconiformes) in Southern Italy. PLoS One, 7(12): e53375. DOI: 10.1371/journal.pone.0053375

Schreven, K., Mooijman, P., Helder, H. (2015): De parasitaire nematode Synhimantus laticeps, geïdentificeerd op basis van de 18S rDNA sequentie, in de maag van een vermagerde Sperwer Accipiter nisus [The parasitic nematode Synhimantus laticeps, identified on the basis of 18S DNA sequence, from the stomach of an emaciated Eurasian sparrwohawk (Accipiter nisus)]. De Takkeling, 23(2): 134 – 140 (In Dutch)

v. Siebold, K.T.E. (1837): Zur Entwicklungsgeschichte der Helminthen [Life history of helminths]. In: Burdach, K.F. Die Physiologie als Erfahrungswissenschaft [Physiology as empirical science]. Zweiter Band. Zweite berichtigte und vermehrte Auflage. Leipzig, Germany: Verlag Leopold Bok, pp. 183 – 213 (In German)

Smogorzhevskaya, L.A. (1990): Nematodes. Part 3. Acuarioidea. In: Sharpilo, V.P. (Ed) Fauna Ukrainy [The Fauna of Ukraine]. Volume 32. Kiev, Ukraine: Naukova Dumka, pp. 1 – 188 (In Russian)

Yamaguti, S. (1961): Systema Helminthum. Volume III. The Nematodes of Vertebrates. Part I. New York, USA, Interscience Publishers Inc., 679 pp.

Zhang, L., Brooks, D.R., Causey, D. (2004): Two species of Synhimantus (Dispharynx) Railliet, Henry and Sisoff, 1912 (Nematoda: Acuarioidea: Acuariidae), in passerine birds from the Area de Conservacion Guanacaste, Costa Rica. J. Parasitol., 90(5): 1133 – 138. DOI: 10.1645/GE-3253

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