New chromosome characteristics of the monozoic tapeworm Caryophyllaeus laticeps (Cestoda, Caryophyllidea)

Open access


The karyotype of a caryophyllidean tapeworm Caryophyllaeus laticeps (Pallas, 1781) from the freshwater bream Abramis brama (L.) caught in the Slovak part of the River Tisa, was described and originally inspected for amount of heterochromatin and its chromosome localization. The chromosome set comprised nine metacentric and one submetacentric (No. 3) pairs (2n = 20). The chromosomes were up to 12.0 ± 2.5 μm long and the mean total length of haploid genome (TLC) reached 80.6 μm that represents one of the highest yet recorded values among tapeworms. C-banding and staining with fl uorescent dyes DAPI and YOYO1 revealed a distinct banding pattern explicitly on chromosomes with centromeric bright heterochromatin bands present on all 10 chromosome pairs; no pair showed any interstitial heterochromatin. A complete course of spermatocyte meiosis and dynamics of nucleolus formation and degradation during meiotic division was described.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • BAZSALOVICSOVÁ E. KRÁLOVÁ-HROMADOVÁ I. BRABEC J. HANZELOVÁ V. OROS M. SCHOLZ T. (2014): Confl ict between morphology and molecular data: a case of the genus Caryophyllaeus (Cestoda: Caryophyllidea) monozoic tapeworms of cyprinid fi shes. Folia Parasitol. 61 (4): 347 - 54. DOI: 10.14411/fp.2014.035

  • BOMBAROVÁ M. VÍTKOVÁ M. ŠPAKULOVÁ M. KOUBKOVÁ B. (2009): Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization. Genome 52: 897 - 903. DOI: 10.1139/G09-063

  • DOS SANTOS GUERRA M. (1986): Reviewing the chromosome nomenclature of Levan et al. Rev. Brasil. Genet. 9 (4): 741 - 743

  • FRYDRYCHOVÁ M. MAREC F. (2002): Repeated losses of TTAGG telomere repeats in evolution of beetles (Coleoptera). Genetica 115 (2): 179 - 187. DOI: 10.1023/A:1020175912128

  • HANZELOVÁ V. OROS M. BARČÁK D. MIKLISOVÁ D. KIRIN D. SCHOLZ T. (2015): Morphological polymorphism in tapeworms: redescription of Caryophyllaeus laticeps (Pallas 1781) (Cestoda: Caryophyllidea) and characterisation of its morphotypes from different fi sh hosts. Syst. Parasitol. 90 (2): 177 - 190. DOI: 10.1007/ s11230-014-9536-x

  • KRÁLOVÁ-HROMADOVÁ I. ŠTEFKA J. ŠPAKULOVÁ M. OROSOVÁ M. BOMBAROVÁ M. HANZELOVÁ V. BAZSALOVICSOVÁ E. SCHOLZ T. (2010): Intraindividual ITS1 and ITS2 ribosomal sequence variation linked with multiple rDNA loci: a case of triploid Atractolytocestus huronensis the monozoic cestode of common carp. Int. J. Parasitol. 40: 175 - 181. DOI: 10.1016/j.ijpara.2009.07.002

  • KRÁLOVÁ-HROMADOVÁ I. BAZSALOVICSOVÁ E. BOROKOVÁ S. HANZELOVÁ V. (2013): Ribosomal ITS2 structure in Caryophyllaeus laticeps and Caryophyllaeus brachycollis (Cestoda: Caryophyllidea) parasites of cyprinid fi sh. Helminthologia 50 (4): 235 - 273. DOI: 10.2478/s11687-013-0135-1

  • MACKIEWICZ J.S. (1994): Order Caryophyllidea van Beneden in Carus 1863. In KHALIL L.F. JONES A. BRAY R.A. (Eds) Keys to the Cestode Parasites of Vertebrates. CAB International Wallingford UK pp. 21 - 43. ISBN 085198 879 2

  • MEDIOUNI J. FUKOVÁ I. FRYDRYCHOVÁ R. DHOUIBI M.H. MAREC F. (2004) Karyotype sex chromatin and sex chromosome differentiation in the carob moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae). Caryologia 57: 184 - 194.

  • OROSOVÁ M. OROS M. (2012): Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea) invasive parasite of carp Cyprinus carpio. Parasitol. Res. 110: 1937 - 1944. DOI: 10.1007/s00436-011-2720-x.

  • OROSOVÁ M. MAREC F. OROS M. XI B.W. SCHOLZ T. (2010a): A chromosome study and 18S rDNA in situ hybridization of Khawia saurogobii (Cestoda: Caryophyllidea). Parasitol. Res. 106: 587 -593. DOI: 10.1007/s00436-009-1702-8

  • OROSOVÁ M. KRÁLOVÁ-HROMADOVÁ I. BAZSALOVICSOVÁ E. ŠPAKULOVÁ M. (2010b) Karyotype chromosomal characteristics of multiple rDNA clusters and intragenomic variability of ribosomal ITS2 in Caryophyllaeides fennica (Cestoda). Parasitol. Int. 59: 351 - 357. DOI: 10.1016/j.parint.2010.04.007

  • PETKEVIČIŪTĖ R. KUPERMAN B.I. (1992) Karyological investigations of Caryophyllaeus laticeps (Pallas 1781) (Cestoda: Caryophyllidea). Folia Parasitol. 39 (2): 115 - 121

  • SHAPIRO J.A. VON STERNBERG R. (2005): Why repetitive DNA is essential to genome function. Biol. Rev. 80: 1 - 24. DOI: 10.1017/ S1464793104006657

  • SUMNER A. T. (1972): A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75 (1): 304-306

  • ŠPAKULOVÁ M. OROSOVÁ M. MACKIEWICZ J.S. (2011): Cytogenetics and Chromosomes of Tapeworms (Platyhelminthes Cestoda). In ROLLINSON D. HAY S.I. (Eds) Adv. Parasitol. Vol. 74. Burlington UK: Academic Press pp. 177 - 230. ISBN: 978-0-12-385897-9

  • WACHTLER F. STAHL A. (1993): The nucleolus: a structural and functional interpretation. Micron 24: 473 - 505. DOI: 10.1016/0968-4328(93) 90026-W

Journal information
Impact Factor

IMPACT FACTOR 2018: 0.731
5-year IMPACT FACTOR: 0.634

CiteScore 2018: 0.8

SCImago Journal Rank (SJR) 2018: 0.398
Source Normalized Impact per Paper (SNIP) 2018: 0.554

Target audience: researchers in the field of human, veterinary medicine and natural science
Cited By
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 131 83 4
PDF Downloads 91 72 0