Cathaemasia hians infection in Black stork in Slovakia: morphological and histopathological study

Open access

Summary

Cathaemasia hians is an obligate trematode parasite of Black storks that are on the List of protected birds in Europe. In the present study, adult trematodes were isolated from the Black stork post mortem and morphological study revealed C. hians species. In total, 10 worms were found in the oesophagus and the ventriculus of the bird. Histopathological examination of the tissue samples of oesophagus, proventriculus and ventriculus was performed on paraffin sections using a set of staining procedures. The sporadic lesions were seen in the tela submucosa of oesophagus containing connective tissue mast cells, eosinophils and heterophils and some foci were surrounded by the fibrous tissue. In addition, a few inflammatory nodules had larval-like material inside, probably being of the same species. There were no visible morphological alterations in the epithelial layer of lamina propria mucosae of proventriculus, rich in goblet cells as well as in the tela submucosa. Majority of trematodes were localized in the ventriculus, where the lamina propria mucosae was damaged or disrupted sporadically. In these sites, in the tela submucosa, a various food-originated inorganic/organic material and eggs of C. hians were deposited, stimulating a weak inflammatory response. Nodules containing larvae were not observed in any of ventriculus tissue layers. This study demonstrated, for the first time, infection with adults of C. hians trematode in the Black stork nesting in Slovakia. The presence of larvae and eggs in the tissues of the upper gastrointestinal tract of bird was associated with mild inflammatory response but feeding behaviour of adult worms in the ventriculus probably contributed to the enhanced susceptibility of the lamina propria mucosae to mechanical damage by inorganic material. Although larval stages have not yet been documented in the intermediate hosts in Slovakia, our report indicates that the life cycle of C. hians might occur in Central Europe.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • ALONSO J.C. ALONSO J.A. CARRASCAL L.M. (1991): Habitat selection by foraging white storks Ciconia ciconia during the breeding season. Can. J. Zoology 69: 1957 - 1962. DOI: 10.1139/z91-270

  • CARRASCAL L.M. BAUTISTA L.M. LÁZARO E. (1993): Geographical variation in the density of the white stork Ciconia ciconia in Spain: infl uence of habitat structure and climate. Biol. Conserv. 65: 83 - 87

  • DING B.A. PIRONE A. LENZI C. XIAOMING N. BAGLINI A. ROMBOLI I. (2011). Histochemical features of the Muscovy duck small intestine during development. Tissue Cell 43: 190 - 195. DOI:10.1016/j. tice.2011.03.001

  • EDELÉNYI B. (1974): Trematodes II. Fauna Hungariae 117. Akadémiai Kiado Budapest 224

  • ENERBACK L. (1966). Mast cells in rat gastrointestinal mucosa. I. Effects of fi xation. Acta Pathol. Microbiol. Scand. 66: 289 - 302.

  • FALTÝNKOVÁ A. NAŠINCOVÁ V. KABLÁSKOVÁ L. (2008): Larval trematodes (Digenea) of planorbid snails (Gastropoda: Pulmonata) in central Europe: A survey of species and key to their identifi cation. Syst. Parasitol. 69: 155 - 178. DOI: 10.1007/s11230-007-9127-1

  • GRABDA-KAZUBSKA B. BAYSSADE-DUFOUR C. KISELIENE V. (1990): Chaemotaxy and excretory system of Echinocercaria choanophila U. Szidat 1936 a larval form of Cathaemasia hians (Rud. 1809) (Trematoda Cathemasiidae). Acta Parasitol. Pol. 35: 97 - 105

  • HENZ B.M. MAURER M. LIPPERT U. WORM M. BABINA M. (2001): Mast cells as initiators of immunity and host defense. Exp. Dermatol. 10: 1 - 10. DOI: 10.1034/j.1600-0625.2001.100101.x

  • HÖFLE U. KRONE O. BLANCO J.M. PIZZARO M. (2003): Chaunocephalus ferox in free-living white storks in Central Spain. Avian Dis. 47: 506 - 512. DOI: 10.1637/0005-2086(2003)047[0506:CFIFWS]2.0. CO:2

  • HRČKOVÁ G. VELEBNÝ S. SOLÁR P. (2010): Dynamics of hepatic stellate cells collagen types I and III synthesis and gene expression of selected cytokines during hepatic fi brogenesis following Mesocestoides vogae (Cestoda) infection in mice. Int. J. Parasitol. 40(2): 163 - 174. DOI: 10.1016/j.ijpara.2009.06.008

  • HURNÍKOVÁ Z. HRČKOVÁ G. AGREN E. KOMOROVÁ P. FORSMAN J. CHOVANOVÁ B. MOLNÁR L. LETKOVÁ V. (2014). First fi nding of Trichinella pseudospiralis in two Tawny Owls (Strix aluco) from Sweden. Helminthologia 50(3): 190 - 197. DOI: 10.2478/s11687-014-0228-5

  • CHERNOGORENKO M.I. (1983): Larval trematodes in molluscs of the Dnieper River and its reservoirs. Kyiv: Nauk dumka 212

  • KARASKA D. (2002): Bocian čierny (Ciconia nigra). In: Danko Š. Darolová A. Krištín A. (Eds) Rozšírenie vtákov na Slovensku. Bratislava Slovakia: Veda pp. 688

  • KÖNIGOVÁ A. MOLNÁR L. HRČKOVÁ G. VÁRADY M. (2013). The fi rst report of serraspiculiasis in Great Tit (Parus major) in Slovakia. Helminthologia 50(4): 254 - 260. DOI: 10.2478/s11687-013-0138-y

  • KOSTADINOVA A. (2005): Family Cathaemasiidae Fuhrmann 1928. In: JONES A. BRAY R.A. GIBSON D.I. (Eds) Keys to the Trematoda. Volume 2. London UK: CABI Publishing and The Natural History Museum pp. 69 - 73

  • LIPTOVSZKY M. MAJOROS G. PERGE E. (2012): Cathaemasia hians in a Black Stork (Ciconia nigra) in Hungary. J. Wildl. Dis. 48(3): 809 - 811. DOI: 10.7589/0090-3558-48.3.809

  • MACKO J.K. (1960): On the fauna of plathelminthes of Black stork - Ciconia nigra. L. Biologia 7: 549 - 552

  • MERINO S. MARTINEZ J. LANZAROT P. CANO L.S. FERNANDEZ-GARCIA M. RODRIGUEZ-CAABEIRO F. (2001): Cathaemasia hians (Trematoda: Cathaemasiidae) infecting Black Stork nestlings (Ciconia nigra) from central Spain. Avian Pathol. 30: 559 - 561. DOI: 10.1080/03079450120078761

  • MEYERHOLZ D.K. GRIFFIN M.A. CASTILOW E.M. VARGA S.M. (2009). Comparison of histochemical methods for murine eosinophil detection in an RSV vaccine-enhanced infl ammation model. Toxicol. Pathology 37: 249 - 255. DOI: 10.1177/0192623308329342

  • OKULEWICZ A. SITKO J. (2012): Parasitic helminthes - probable cause of death of birds. Helminthologia 49(4): 241 - 246. DOI: 10.2478/s11687-012-0045-7

  • REEVES H.L. FRIEDMAN S.L. (2002): Activation of hepatic stellate cells - a key issue in liver fi brosis. Front. Biosci. 7: d808 - 826

  • SANTORO M. BEGLI UBERTI B. GALIERO G. DI PRISCO F. ALESSIO N.D. VENEZIANO V. (2013): Chaunocephalus ferox (Digenea: Echinostomatidae) infection associated with fatal cachexia in a white stork (Ciconia ciconia). Helminthologia 50(3): 181 - 184. DOI: 10.2478/s11687-013-0129-z

  • SEY O. (1965): Studies on endoparasitic helminths of Hungary. Acta Acad. Paedag. Civ. Pecs 9: 179 - 199

  • SCHUSTER R. SCHAFFER T. SHIMALOV V. (2002): Die Helminthenfauna einhemisher Weisstőrche (Ciconia ciconia). Berl. Munch. Tierarztl. Wochenschr. 115: 435 - 439

  • SITKO J. HENEBERG P. (2015). Composition structure and pattern of helminth assemblages associated with central European storks (Ciconiidae). Parasitol. Int. 64: 130 - 134. DOI: org/10.1016/j. parint.2014.11.004

  • TURK D.E. (1982). Symposium: The avian gastrointestinal tract and digestion. The anatomy of the avian digestive tract as related to feed utilization. Poultry Sci. 61: 1225 - 1244

  • ZHYTOVA O.P. KOROL E.M. (2012): Cathaemasia hians (Digenea Cathaemasiidae) from Planorbis planorbis (Mollusca Gastropoda) in reservoirs of central Polissya. Vest. zool. 46(4): e-42 - e-45. DOI: 10.2478/v10058-012-0029-6

Search
Journal information
Impact Factor

IMPACT FACTOR 2018: 0.731
5-year IMPACT FACTOR: 0.634

CiteScore 2018: 0.8

SCImago Journal Rank (SJR) 2018: 0.398
Source Normalized Impact per Paper (SNIP) 2018: 0.554

Target audience: researchers in the field of human, veterinary medicine and natural science
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 220 144 4
PDF Downloads 130 87 0