Use of modified McMaster method for the diagnosis of intestinal helminth infections and estimating parasitic egg load in human faecal samples in non-endemic areas

Open access

Abstract

A modified McMaster method has been used for the diagnosis and estimating helminth egg load in human faecal samples obtained from random consecutive patients in the areas non-endemic for helminth infections (Slovak Republic, North West Russia). Both positive and negative findings were in a 100 % concordance to those obtained with a reference method accepted in clinical diagnostic laboratories (microscopy of the native stool smear). The McMaster method was efficient in detecting nematode eggs in patients’ stool samples with egg load varying from very low (15–60 epg for T. trichiura) to moderate (1650–4500 epg for A. lumbricoides). Therefore, this method may be successfully (and with a better technical feasibility) used for the diagnosis of intestinal helminth infections in non-endemic areas, with further quantitative analysis of the sample when required.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1] Bekele T. (2002): Epidemiological studies on gastrointestinal helminths of dromedary (Camelus dromedarius) in semi-arid lands of eastern Ethiopia. Vet. Parasitol. 105(2): 139–152 http://dx.doi.org/10.1016/S0304-4017(01)00583-0

  • [2] Bell W. J. Nassif S. (1971): Comparison of pyrantel pamoate and piperazine phosphate in the treatment of ascariasis. Am. J. Trop. Med. Hyg. 20(4): 584–588

  • [3] Cebra C. K. Stang B. V. (2008): Comparison of methods to detect gastrointestinal parasites in llamas and alpacas. J. Am. Vet. Med. Assoc. 232(5): 733–741 http://dx.doi.org/10.2460/javma.232.5.733

  • [4] Coles G. C. Bauer C. Borgsteede F. H. M. Geerts S. Klei T. R. Taylor M. A. Waller P. J. (1992): World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44(1): 35–44 http://dx.doi.org/10.1016/0304-4017(92)90141-U

  • [5] Cringoli G. Rinaldi L. V. Veneziano V. Capelli G. Scala A. (2004): The influence of flotation solution sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Vet. Parasitol. 123(1): 121–131 http://dx.doi.org/10.1016/j.vetpar.2004.05.021

  • [6] Dunn A. Keymer A. (1986): Factors affecting the reliability of the McMaster technique. J. Helminthol. 60(4): 260–262 http://dx.doi.org/10.1017/S0022149X00008464

  • [7] Flohr C. Tuyen L. N. Lewis S. Tan Minh T. Campbell J. Britton J. Williams H. Tinh Hien T. Farrar J. Quinnel R. J. (2007): Low efficacy of mebendazole against hookworm in Vietnam: two randomized controlled trials. Am. J. Trop. Med. Hyg. 76(4): 732–736

  • [8] Giannetto S. Brianti E. Poglayen G. Sorgi C. Capelli G. Pennisi M. G. Coci G. (2007): Efficacy of oxfendazole and fenbendazole against tortoise (Testudo hermanni) oxyurids. Parasitol. Res. 100(5): 1069–1073 http://dx.doi.org/10.1007/s00436-006-0361-2

  • [9] Groth T. (1990): Some methodological aspects on quality assurance in laboratory medicine. Upsala J. Med. Sci. 95(3): 265–273 http://dx.doi.org/10.3109/03009739009178602

  • [10] Rinaldi L. Russo T. Schioppi M. Pennacchio S. Cringoli G. (2007): Passalurus ambiguus: new insights into copromicroscopic diagnosis and circadian rhythm of egg excretion. Parasitol. Res. 101(2): 557–561 http://dx.doi.org/10.1007/s00436-007-0513-z

  • [11] Marshall W. J. (2000): Clinical Chemistry: Fourth edition. Mosby Publ. Edinburgh et al.

  • [12] Meyer-Lucht Y. Sommer S. (2005): MHC diversity and the association to nematode parasitism in the yellow-necked mouse (Apodemus flavicollis). Mol. Ecol. 14(7): 2233–2243 http://dx.doi.org/10.1111/j.1365-294X.2005.02557.x

  • [13] Sargent R. G. Dotterer T. D. Savory A. M. Lee P. R. (1975): A clinical evaluation of the efficacy of mebendazole in the treatment of trichuriasis. South. Med. J. 68(1): 38–40

  • [14] Schad S. J. Ganzhorn J. U. Sommer S. (2004): Parasite burden and constitution of major histocompatibility complex in the Malagasy mouse lemur Microcebus murinus. Evolution 59(2): 439–450

  • [15] Seivwright L. J. Redpath S. M. Mougeot F. Watt L. Hudson P. J. (2004): Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagopus scoticus. J. Helminthol. 78(1): 69–76 http://dx.doi.org/10.1079/JOH2003220

  • [16] Stephenson L. S. Latham M. C. Kurz K. M. Kinoti S. N. Brigham H. (1989): Treatment with a single dose of albendazole improves growth of Kenyan schoolchildren with hookworm Trichuris trichiura and Ascaris lumbricoides infections. Am. J. Trop. Med. Hyg. 41(1): 78–87

  • [17] Thienpont D. Brugmans J. Abadi K. Tanamal S. (1969): Tetramizole in the treatment of nematode inections in man. Am. J. Trop. Med. Hyg. 18(4): 520–525

Search
Journal information
Impact Factor

IMPACT FACTOR 2018: 0.731
5-year IMPACT FACTOR: 0.634

CiteScore 2018: 0.8

SCImago Journal Rank (SJR) 2018: 0.398
Source Normalized Impact per Paper (SNIP) 2018: 0.554

Target audience: researchers in the field of human, veterinary medicine and natural science
Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 192 122 4
PDF Downloads 90 62 4