Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

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Abstract

Toxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

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  • [1] Borecka A. (2004): Differentiation of Toxocara spp. eggs isolated from the soil by PCR-linked RFLP. Helminthologia 41: 185–187

  • [2] Cho S. Egami M. Ohnuki H. Saito Y. Chinone S. Shichinohe K. Suganuma M. Akao N. (2007): Migration behaviour and pathogenesis of five ascarid nematode species in the Mongolian gerbil Mariones unguiculatus. J. Helminth; 81: 43–47 http://dx.doi.org/10.1017/S0022149X07212118

  • [3] Jacobs D. E. Zhu X. Gasser R. B. Chilton N. B. (1997): PCR-based methods for identification of potentially zoonotic ascaridoid parasites of the dog fox and cat. Acta Trop. 68: 191–200 http://dx.doi.org/10.1016/S0001-706X(97)00093-4

  • [4] Kennedy M. Qureshi W. F. Fraser E. M. Haswellelkins M. R. Elkins O. B. Smith H. V. (1989): Antigenic relationship between the surface exposed secreted and somatic materials of the nematode parasites Ascaris lumbricoides Ascaris suum and Toxocara canis. Clin. Exp. Immunol. 75: 493–500

  • [5] Magnaval J. F. Fabre R. Maurieres P. Charlet J. P. De Larrard B. (1991): Application of the western-blotting procedure for the immunodiagnosis of human toxocariasis. Parasite Res. 77: 687–702

  • [6] Magnaval J. F. Glickman L. T. Dorchies P. Morassin B. (2001a): Highlights of human toxocariasis. Kor. J. Parasitol. 39: 1–11

  • [7] Magnaval J. F. Berry A. Fabre R. Morassin B. (2001b): Eosinophil cationic protein as a possible marker of active human Toxocara infection. Allergy 56: 1096–1099 http://dx.doi.org/10.1034/j.1398-9995.2001.00284.x

  • [8] Obwaller A. Jensen-Jarolim E. Auer H. Huber A. Q. Kraft D. Aspöck H. (1998): Toxocara infestations in humans: symptomatic course of toxocarosis correlates significantly with levels of IgE/anti-IgE immune complexes. Parasite Immunol. 20: 311–317 http://dx.doi.org/10.1046/j.1365-3024.1998.00146.x

  • [9] Thompson D. E. Bundy D. A. P. Cooper E. S. Schantz P. M. (1986): Epidemiological characteristics of Toxocara canis zoonotic infection of children in a Caribbean community. Bull. World Health Organ. 64: 283–290

  • [10] Zhu X. Q. Jacobs D. E. Chilton N. B. Sani R. A. Cheng N. A. B. Y. Gasser R. B. (1998): Molecular characterization of a Toxocara variant from cats in Kuala Lumpur Malaysia. Parasitology 117: 155–164 http://dx.doi.org/10.1017/S0031182098002856

  • [11] Żarnowska H. Jastrzębska M. (1994): Excretorysecretory larval antigens of Toxocara canis: physico-chemical characterisation and specificity assayed by Western blot technique. Acta Parasitol. 39: 41–45

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