The first systematic research on canine dirofilariosis in Slovakia started in February 2007. In total 287 dogs of various age, sex, breeds, and utilization from southern Slovakia were examined within the study until September 2007. Microfilariaemia was detected in 99 (34.5 %) blood samples. Histochemical staining and PCR approach were used for Dirofilaria species identification. Dirofilaria repens was confirmed in all infected dogs; it occurred in 92 (32.1 %) dogs as a single infection and in 6 (2.1 %) dogs in mixed infection with Dirofilaria immitis. For the first time both species were detected in Eastern Slovakia. Outdoor keeping and age higher 3 years become evident as important risk factors. Male individuals and dogs with long hair were significantly more frequently infected when compared with females and short hair breeds.
The present study revealed the occurrence of highly endemic area of dirofilariosis in Slovakia and certified that climatic conditions in the country are favourable for development of the parasite and for further spread of this zoonotic infection.
If the inline PDF is not rendering correctly, you can download the PDF file here.
 Anderson R. C. (2000): Nematode parasites of vertebrates their development and transmission. 2nd Edition CABI Publishing Wallingford UK 650 p.
 Cancrini G. Sun Yanchang Della Torre A. Coluzzi M. (1988): Influenza della temperatura sullo sviluppo larvale di Dirofilaria repens in diverse specie di zanzare. Parassitologia 30: 38
 Cringoli G. Rinaldi L. Veneziano V. Capelli G. (2001): Prevalence survey and risk analysis of filariosis in dogs from the Mt. Vesuvius area of southern Italy. Vet. Parasitol. 102: 243–252 http://dx.doi.org/10.1016/S0304-4017(01)00529-5
 Dobešová R. Svobodová Z. Svobodová V. (2007): Dirofilariosis in dogs-the actual situation in the Czech Republic. In Genchi C. Rinaldi L. Cringoli G. (Eds.): Mappe Parassitologie — Dirofilaria immitis and Dirofilaria repens in dog and cat and human infection 8:198
 Fortin J. F. Slocombe J. O. D. (1981): Temperature requirements for the development of Dirofilaria immitis in Aedes triseriatus and Ae. vexans. Mosq. News 41: 625–633
 Fok É. Kiss G. Majoros G. Jacsó O. Farkas R. Gyurkovszky M. (2007): Preliminary results of an epidemiological survey on dirofilariosis of dogs and cats in Hungary. In Genchi C. Rinaldi L. Cringoli G. (Eds.): Mappe Parassitologie-Dirofilaria immitis and Dirofilaria repens in dog and cat and human infection 8: 195
 Genchi C. Solari Basano G. Bandi C. Di Sacco B. Venco L. Vezzoni A. Cancrini G. (1995): Factors influencing the spread of heartworms in Italy: interaction between Dirofilaria immitis and Dirofilaria repens. Proceedings of Heartworm Symposium’ 95. American Heartworm Society Baravia IL. 65–71
 Genchi C. Rinaldi L. Cascone C. Mortarino M. Cringoli G. (2005): Is heartworm disease really spreading in Europe? Vet. Parasitol. 133(2): 137–148 http://dx.doi.org/10.1016/j.vetpar.2005.04.009
 Georgieva D. Kirkova Z. Ivanov A. (2001): A study on the incidence and diagnostic of dirofilariosis (heartworm disease) in carnivores. Bulgarian J. Vet. Med. 4: 231–236
 Jallili N. Kobzová D. Novák I. Dubinský P. Babál P. (2007): The first case of human dirofilariosis in Slovakia (in Slovak with English abstract). Acta Tropica 5: 75–78
 Knott J. (1939): A method for making microfilarial surveys on day blood. Trans. R. Soc. Trop. Med. Hyg. 33: 191 http://dx.doi.org/10.1016/S0035-9203(39)90101-X
 Magi M. Marroncini A. Sassetti M. (1989): Distribution of canine filariasis in Tuscany. Sel. Vet. 30: 1185–1187
 Montoya J. A. Morales M. Ferrer O. Molina J. M. Corbera J. A. (1998): The prevalence of Dirofilaria immitis in Gran Canaria Canary Islands Spain (1994–1996). Vet. Parasitol. 75: 221–226 http://dx.doi.org/10.1016/S0304-4017(97)00175-1
 Peribanez M. A. Lucientes J. Arce S. Morales M. Castillo J. A. Gracia M. J. (2001): Histochemical differentiation of Dirofilaria immitis Dirofilaria repens and Acanthocheilonema dracunculoides microfilariae by staining with a commercial kit Leucognost-SP®. Vet. Parasitol. 102: 173–175 http://dx.doi.org/10.1016/S0304-4017(01)00516-7
 Poglayen G. Martini M. Bomben L. Roda R. (1996): An update of the occurrence of canine heartworm disease in northern Italy. Vet. Res. Commun. 20: 303–307 http://dx.doi.org/10.1007/BF00366535
 Rishniw M. Barr S. C. Simpson K. W. Frongillo M. Franz M. Dominquez Alpizar J. L. (2006): Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet. Parasitol. 135(3): 303–314 http://dx.doi.org/10.1016/j.vetpar.2005.10.013
 Scala A. Atzori F. Varcasia A. Grippa G. Genchi C. (2004): Filariosis in Sardinia: epidemiological updating (1998–2004). Atti SISVET 58: 120–122
 Statsoft Inc: STATISTICA v. 6 2001 http://www.statsoft.com
 Svobodová Z. Svobodová V. Genchi C. (2007): Dirofilaria repens infection in dogs in the Czech Republic. In Genchi C. Rinaldi L. Cringoli G. (Eds.): Mappe Parassitologie — Dirofilaria immitis and Dirofilaria repens in dog and cat and human infection 8: 199
 Svobodová V. Svobodová Z. Beladičová V. Valentová D. (2005): First case of canine dirofilariosis in Slovakia: a case report. Vet. Med. Czech. 50(11): 510–512
 Webber W. A. F. Hawking F. (1955): Experimental maintenance of Dirofilaria repens and D. immitis in dogs. Exp. Parasitol. 4: 143–164 http://dx.doi.org/10.1016/0014-4894(55)90007-2