Appraisal of the Antioxidative Potential of Aloe Barbadensis M. on Alcohol-Induced Oxidative Stress

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Abstract

This investigation estimated the anti-oxidative potential of Aloe barbadensis gel extracts in rats against alcohol-induced oxidative stress. Thirty male albino rats (5 each per group) were included in the experiments. Group A (positive control) and B (negative control) were administered 4 mg.kg–1 body weight distilled water and 50 % alcohol respectively for 21 days. Groups C and D were administered 50 % alcohol for the first 14 days followed by co-administration of 125 mg and 250 mg.kg−1 body weight extract with alcohol respectively for the last 7 days. Groups E and F were administered distilled water for the first 14 days followed by co-administration of 125 and 250 mg.kg−1 body weight Aloe barbadensis gel extracts with distilled water respectively for the last 7 days. The administration of alcohol resulted in a significant (P < 0.05) decrease in the specific activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) levels, while cholesterol (CHO), triglycerides (TAG), nitric oxide (NO) and malondialdehyde (MDA) concentrations were significantly increased when compared to the controls. Co-mobilization with Aloe barbadensis gel extracts for 7 days significantly reversed the deleterious effects of alcohol in the treated groups when compared to the alcohol group. This study indicated that Aloe barbadensis probably possesses anti-oxidative effects against alcohol–induced oxidative stress in rats.

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  • 1. Al-Shinnawy M. S. Hassan A. R. Ismail D. A. Shahin M. A. 2014: The potential protective and therapeutic effects of aloe vera juice on malathion induced hepatotoxicity in rabbits. The Egyptian Journal of Hospital Medicine 55 146—158. DOI: 10.12816/0004500

  • 2. Awazawa M. Ueki K. Inabe K. Yamauchi T. Kaneko K. Okazaki Y. et al. 2009: Adiponectin suppresses hepatic SREBP1c expression in an AdipoR1/LKB1/AMPK dependent pathway. Biochemical and Biophysical Research Communications 382 1 51—56. DOI: 10.1016/j.bbrc.2009.02.131.

  • 3. Bashir A. Saeed B. Mujahid T. Y. Jehan N. 2011: Comparative study of antimicrobial activities of Aloe vera extracts and antibiotics against isolates from skin infections. African Journal of Biotechnology 10 19 3835—3840. DOI: 10.5897/AJB07.572.

  • 4. Brown L. A. S. Harris F. L. Ping X D. Gauthier T. W. 2004: Chronic ethanol ingestion and the risk of acute lung injury: a role for glutathione availability ? Alcohol 33 3 191—197. DOI: 10.1016/j.alcohol.2004.08.002.

  • 5. Calberg I. Mannervick B. 1985: Glutathione reductase. Method Enzymol. 113 485—490.

  • 6. Carney J. M. Starke-Reed P. E. Oliver C. N. Landum R. W. Cheng M. S. Wu J. F. Floyd R. A. 1991: Reversal of age-related increase in brain protein oxidation decrease in enzyme activity and loss in temporal and spatial memory by chronic administration of the spin-trapping compound N-tert-butyl-alpha-phenylnitrone. Proceedings of the National Academy of Sciences 88 9 3633—3636. DOI: 10.1073/pnas.88.9.3633.

  • 7. Chandan B. K. Saxena A. K. Shukla S. Sharma N. Gupta D. K. Suri K. A. et al. 2007: Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity. Journal of Ethnopharmacology 111 3 560—566. DOI: 10.1016/j.jep.2007.01.008.

  • 8. Chen L. Wang F. Sun X. Zhou J. Gao L. Jiao Y. et al. 2010: Chronic ethanol feeding impairs AMPK and MEF2 expression and is associated with GLUT4 decrease in rat myocardium. Experimental and Molecular Medicine 42 3 205—215. DOI: 10.3858/emm.2010.42.3.021.

  • 9. Chinedu E. Arome D. Ameh F. S. 2013: A new method for determining acute toxicity in animal models. Toxicology International 20 3 224—226. DOI: 10.4103/0971-6580.121674.

  • 10. Chun-hui L. Chang-hai W. Zhi-liang X. Yi W. 2007: Isolation chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water. Process Biochemistry 42 6 961—970. DOI: 10.1016/j.procbio.2007.03.004.

  • 11. Cui Y. Ye Q. Wang H. Li Y. Yao W. Qian H. 2014: Hepatoprotective potential of Aloe vera polysaccharides against chronic alcohol-induced hepatotoxicity in mice. Journal of the Science of Food and Agriculture 94 9 1764—1771. DOI: 10.1002/jsfa.6489.

  • 12. Debnath T. Ghosh M. Lee Y. M. Nath N. C. D. Lee K. G. Lim B. O. 2018: Identification of phenolic constituents and antioxidant activity of Aloe barbadensis flower extracts. Food and Agricultural Immunology 29 1 27—38. DOI:10.1080/09540105.2017.1358254.

  • 13. Deleve L. D. Kaplowitz N. 1990: November. Importance and regulation of hepatic glutathione. In Seminars in Liver Disease Thieme Medical Publishers Inc. 10 4 251—266.

  • 14. Donohue Jr. T. M. 2007: Alcohol-induced steatosis in liver cells. World Journal of Gastroenterology 13 37 4974—4978. DOI: 10.3748/wjg.v13.i37.4974.

  • 15. Dosumu O. O. Osinubi A. A. A. Duru F. I. O. 2014: Alcohol induced testicular damage: Can abstinence equal recovery ? Middle East Fertility Society Journal 19 3 221—228. DOI: 10.1016/j.mefs.2014.01.003.

  • 16. Dupont I. Bodenez P. Berthou F. Simon B. Bardou L. G. Lucas D. 2000: Cytochrome P-450 2E1 activity and oxidative stress in alcoholic patients. Alcohol and Alcoholism 35 1 98—103. DOI: 10.1093/alcalc/35.1.98.

  • 17. Fraga C. G. Leibovitz B. E. Tappel A. L. 1988: Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radical Biology and Medicine 4 3 155—161. DOI: 10.1016/0891-5849(88)90023-8.

  • 18. Gardner C. D. Coulston A. Chatterjee L. Rigby A. Spiller G. Farquhar J. W. 2005: The effect of a plant-based diet on plasma lipids in hypercholesterolemic adults: a randomized trial. Annals of Internal Medicine 142 9 725—733. DOI: 10.7326/0003-4819-142-9-200505030-00007.

  • 19. Gidez L. I. Miller G. J. Burstein M. Slagle S. Eder H. A. 1982: Separation and quantitation of subclasses of human plasma high density lipoproteins by a simple precipitation procedure. Journal of Lipid Research 23 8 1206—1223.

  • 20. Ginsberg H. N. 1994: Lipoprotein metabolism and its relationship to atherosclerosis. The Medical Clinics of North America 78 1 1—20. DOI: 10.1016/S0025-7125(16)30174-2.

  • 21. Glew R. H. Kassam H. A. Bhanji R. A. Okorodudu A. VanderJagt D. J. 2002: Serum lipid profiles and risk of cardiovascular disease in three different male populations in northern Nigeria. Journal of Health Population and Nutrition 20 166—174.

  • 22. Hadwan M. H. Abed H. N. 2016: Data supporting the spec-trophotometric method for the estimation of catalase activity. Data in Brief 6 194—199. DOI: 10.1016/j.dib.2015.12.012.

  • 23. Halliwell B. 1987: Oxidants and human disease: some new concepts. The FASEB Journal 1 5 358—364. DOI: 10.1096/fasebj.1.5.2824268.

  • 24. Hu S. Yin S. Jiang X. Huang D. Shen G. 2009: Melatonin protects against alcoholic liver injury by attenuating oxidative stress inflammatory response and apoptosis. European Journal of Pharmacology 616 1—3 287—292. DOI: 10.1016/j.ejphar.2009.06.044.

  • 25. Johnsen-Soriano S. Genovés J. M. Romero B. García-Delpech S. Muriach M. Sancho-Tello M. et al. 2007: Chronic ethanol feeding induces oxidative stress in the rat retina: treatment with the antioxidant ebselen. Archivos de la Sociedad Espanola de Oftalmologia 82 12 757—762.

  • 26. Josias H. H 2008: Composition and applications of Aloe vera leaf gel. Molecules 13 8 1599—1616. DOI: 10.3390/molecules13081599.

  • 27. Kukielka E. Dicker E. Cederbaum A. I. 1994: Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment. Archives of Biochemistry and Biophysics 309 2 377—386. DOI: 10.1006/abbi.1994.1127.

  • 28. Lakshmi P. T. V. Rajalakshmi P. 2011: Identification of phyto components and its biological activities of Aloe vera through the gas chromatography-mass spectrometry. Int. Res. J. Pharm. 2 5 247—249.

  • 29. Lampe M. A. Burlingame A. L. Whitney J. Williams M. L. Brown B. E. Roitman E. Elias P. M. 1983: Human stratum corneum lipids: characterization and regional variations. J. Lipid Res. 24 2 120—130.

  • 30. Lee H. I. McGregor R. A. Choi M. S. Seo K. I. Jung U. J. Yeo J. et al. 2013: Low doses of curcumin protect alcohol-induced liver damage by modulation of the alcohol metabolic pathway CYP2E1 and AMPK. Life Sciences 93 18—19 693—699. DOI: 10.1016/j.lfs.2013.09.014.

  • 31. Li S. Y. Gomelsky M. Duan J. Zhang Z. Gomelsky L. Zhang X. Epstein P. N. Ren J. 2004: Overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene prevents acetaldehyde-induced cell injury in human umbilical vein endothelial cells role of ERK and p38 mitogen-activated protein kinase. Journal of Biological Chemistry 279 12 11244—11252. DOI: 10.1074/jbc.M308011200.

  • 32. Lichtenstein A. H. Appel L. J. Brands M. Carnethon M. Daniels S. Franch H. A. et al. N. 2006: Diet and lifestyle recommendations revision 2006: a scientific statement from the American Heart Association Nutrition Committee. Circulation 114 1 82—96. DOI: 10.1161/CIRCULATIONAHA.106.176158.

  • 33. Lieber C. S. 2004: Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol 34 1 9—19. DOI: 10.1016/j.alcohol.2004.07.008.

  • 34. Marklund S. Marklund G. 1974: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47 3 469—474. DOI: 10.1111/j.1432-1033.1974.tb03714.x.

  • 35. McKim S. E. Gäbele E. Isayama F. Lambert J. C. Tucker L. M. Wheeler M. D. et al. 2003: Inducible nitric oxide synthase is required in alcohol-induced liver injury: studies with knockout mice. Gastroenterology 125 6 1834—1844. DOI: 10.1053/j.gastro.2003.08.030.

  • 36. Moron M. S. Depierre J. W. Mannervik B. 1979: Levels of glutathione glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects 582 1 67—78. DOI: 10.1016/0304-4165(79)90289-7.

  • 37. National Research Council 2006: Guide for the Care and Use of Laboratory Animals 8th edn. The National Academic Press Washington DC 41—103.

  • 38. Naveena Bharath B. K. Selvasubramanyam 2011: Antitumor activity of Aloe vera against Ehrlich ascitis carcinoma in Swiss albino mice. International Journal of Pharma and Bioscience 2 2 400—409.

  • 39. Nguyen K. H. Lee J. H. Nyomba B. G. 2012: Ethanol causes endoplasmic reticulum stress and impairment of insulin secretion in pancreatic β-cells. Alcohol 46 1 89—99. DOI: 10.1016/j.alcohol.2011.04.001.

  • 40. Padmanabhan P. Jangle S. N. 2014: Hepatoprotective activity of herbal preparation (HP-4) against alcohol induced hepatotoxicity in mice. Int. J. Appl. Sci. Biotech. 2 1 50—58. DOI: 10.3126/ijasbt.v2i1.9346.

  • 41. Pal S. Bhattacharjee A. Mukherjee S. Bhattacharya K. Mukherjee S. Khowala S. 2014: Effect of Alocasia indica tuber extract on reducing hepatotoxicity and liver apoptosis in alcohol intoxicated rats. BioMed Res. Int. 2014 10 pp. DOI: 10.1155/2014/349074.

  • 42. Pandanaboina S. C. Kondeti S. R. Rajbanshi S. L. Kunala P. N. Pandanaboina S. Pandanaboina M. M. Wudayagiri R. 2012: Alterations in antioxidant enzyme activities and oxidative damage in alcoholic rat tissues: Protective role of Thespesia populnea. Food Chemistry 132 1 150—159. DOI: 10.1016/j.foodchem.2011.10.046.

  • 43. Rotruck J. T. Pope A. L. Ganther H. E. Swanson A. B. Hafeman D. G. Hoekstra W. 1973: Selenium: biochemical role as a component of glutathione peroxidase. Science 179 4073 588—590. DOI: 10.1126/science.179.4073.588.

  • 44. Saljooghianpour M. Javaran T. A. 2013: Identification of phytochemical components of aloe plantlets by gas chromatography-mass spectrometry. African Journal of Biotechnology 12 49 6876—6880. DOI: 10.5897/AJB11.4307.

  • 45. Sathyaprabha G. Kumaravel S. Ruffina D. Praveenkumar P. 2010: A comparative study on antioxidant proximate analysis antimicrobial activity and phytochemical analysis of Aloe vera and Cissus quadrangularis by GC-MS. Journal of Pharmacy Research 3 12 2970—2973.

  • 46. Scott R. B. Reddy K. S. Husain K. Schlorff E. C. Rybak L. P. Somani S. M. 2000: Dose response of ethanol on anti-oxidant defense system of liver lung and kidney in rat. Patho-physiology 7 1 25—32. DOI: 10.1016/S0928-4680(99)00034-6.

  • 47. Sild E. Hõrak P. 2009: Nitric oxide production: an easily measurable condition index for vertebrates. Behavioral Ecology and Sociobiology 63 6 959—966. DOI: 10.1007/s00265-009-0710-0.

  • 48. Szabo G. Bala S. 2010: Alcoholic liver disease and the gut-liver axis. World Journal of Gastroenterology: WJG 16 11 1321—1329. DOI: 10.3748/wjg.v16.i11.1321.

  • 49. Tajoda H. N. Kurian J. C. Bredenkamp M. B. 2013: Reduction of cholesterol and triglycerides in volunteers using lemon and apple. Int. J. Humanit. Soc. Sci. 3 18 60—64.

  • 50. Van der Gaag M. S. Van Tol A. Vermunt S. H. F. Scheek L. M. Schaafsma G. Hendriks H. F. J. 2001: Alcohol consumption stimulates early steps in reverse cholesterol transport. Journal of Lipid Research 42 12 2077—2083.

  • 51. Vogt B. L. Richie Jr. J. P. 2007: Glutathione depletion and recovery after acute ethanol administration in the aging mouse. Biochemical Pharmacology 73 10 1613—1621. DOI: 10.1016/j.bcp.2007.01.033

  • 52. Zima T. Fialová L. Mestek O. Janebová M. Crkovská J. Malbohan I. et al. 2001: Oxidative stress metabolism of ethanol and alcohol-related diseases. Journal of Biomedical Science 8 1 59—70. DOI: 10.1007/BF02255972.

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