Immunohistochemical Study of the Stromal Cells in the Lactating Bovine Mammary Gland

Open access


The bovine mammary gland is a special gland characterized by high secretory activity. During lactation the cellular and fibrous components of the interstitial tissue septa are exposed to store accumulated secretory products. The aim of this study was to find and study the cells in the stroma of the bovine lactating mammary gland. For this purpose, the immunohistochemical methods and antibodies against the smooth muscle actin, vimentin, and desmin were used. The myoepithelial cells (MEC) which stained with smooth muscle actin (SMA), were found supporting the secretory units and the intralobular ducts. Coexpression of the SMA and desmin were found in the smooth muscle cells of the blood vessels. The fibroblasts (myofibroblasts) and free cells positive to vimentin were located in the connective tissue septa. The results of this study on the mammary glands indicated that smooth muscle cells (SMC) were altered in the lactating mammary gland, with additional cells such as fibroblasts (myofibroblasts) participated in the storage and after milk let-down they allowed the mammary glands to return to their original state.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Adriance M. C. Inman J. L. Petersen O. W. Bissell M. J. 2005: Myoepithelial cells: good fences make good neighbors. Breast Cancer Res. 7 190—197.

  • 2. Alkafafy M. Rashedb R. Helalc A. 2011: Immunohistochemical studies on the bovine lactating mammary gland (Bos taurus). Acta Histochemica 114 87—89.

  • 3. Destexhe E. Lespagnard L. Degeyter M. Heymann R. Coignoul F. 1993: Immunohistochemical identification of myoepithelial epithelial and connective tissue cells in canine mammary tumors. Vet. Pathol. 30 146—154.

  • 4. Faraldo M. M. Taddei-De La Hosseraye I. Teulière J. 2006: Mammary gland development: role of basal myoepithelial cells. J. Soc. Biol. 200 193—198.

  • 5. Foschini M. P. Scarpellini F. Gown A. M. Eusebi V. 2000: Differential expression of myoepithelial markers in salivary sweat and mammary glands. Int. J. Surg. Pathol. 8 29—37.

  • 6. Gould V. E. Koukoulis G. K. Jansson D. S. Nagle R. B. Franke W. W. Moll R. 1990: Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal hyperplastic and neoplastic breast. Amer. J. Pathol. 137 1143—1155.

  • 7. Gulbahar M. Y. Guvenc T. Yarim M. Kabak Y. B. Sozgen Y. 2007: Mammary fibroadenoma in a lamb. J. Vet. Sci. 8 423—425.

  • 8. Ingman W. V. Wyckoff J. Gouon-Evans V. Condeelis J. Pollard J. W. 2006: Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland. Dev. Dynam. 235 3222—3229.

  • 9. Jiang W. G. Grimshaw D. Martin T. A. Davies G. Parr C. Watkins G. Abounader R. Laterra J. Mansel R. E. 2003: Reduction of stromal fibroblast-induced mammary tumor growth by retroviral ribozyme transgenes to hepatocyte growth factor/scatter factor and its receptor c-MET. Clin. Cancer Res. 9 4274—4281.

  • 10. Lazard D. Sastret X. Frid M. G. Glukhova M. A. Thiery J. P. Koteliansky V. E. 1993: Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue. Proc. Natl. Acad. Sci. 90 999—1003.

  • 11. Marettová E. Maretta M. Legáth J. 2007: Immunohistochemical detection of smooth muscle actin in the sheep mandibular and parotid salivary gland. Folia Veterinaria 51 122—125.

  • 12. Michalczyk A. Brown R. W. Collins J. P. Ackland M. L. 2001: Lactation affects expression of intermediate filaments in human breastepithelium. Differentiation 67 41—49.

  • 13. Moisan E. Girard D. 2006: Cell surface expression of intermediate filament proteins vimentin and lamin B1 in human neutrophil spontaneous apoptosis. J. Leukocyte Biol. 79 489—498.

  • 14. Mor-Vaknin N. Punturieri A. Sitwala K. Markovitz D. M. 2003: Vimentin is secreted by activated macrophages. Nat. Cell. Biol. 5 59—63.

  • 15. Niveditha S. R. Bajaj P. 2003: Vimentin expression in breast carcinomas. Indian J. Pathol. Microbiol. 46 579—584.

  • 16. Nico B. Ennas M. G. Crivellato E. Frontino A. Mangieri D. De Giorgis M. Roncali L. Ribatti D. 2004: Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2. Microvasc. Res. 68 13—19.

  • 17. O’Brien J. Lyons T. Monks J. Lucia M. S. Wilson R. S. Hines L. Schedin P. 2010: Alternatively activated macrophages and collagen remodeling characterize the postpartum involuting mammary gland across species. Amer. J. Pathol. 176 1241—1255.

  • 18. Powell D. W. Mifflin R. C. Valentich J. D. Crowe S. E. Saada J. I. West A. B. 1999: Myofibroblasts. I. Paracrine cells important in health and disease. Am. J. Physiol. 277 C1—C19.

  • 19. Rabanal R. M. Else R. W. 1994: Immunohistochemical localisation of cytokeratin and vimentin intermediate filament proteins in canine mammary tumours. Res. Vet. Sci. 56 225—233.

  • 20. Radu E. Regalia T. Ceafalan L. Andrei F. Cretoiu D. Popescu L. M. 2005: Cajal-type cells from human mammary gland stroma: phenotype characteristics in cell culture. J. Cell. Mol. Med. 9 748—751.

  • 21. Rangdaeng S. Truong L. D. 1991: Comparative immunohistochemical staining for desmin and muscle-specific actin. A study of 576 cases. Am. J. Clin. Pathol. 96 32—45.

  • 22. Richardson K. C. 2009: Contractile tissues in the mammary gland with special reference to myoepithelium in the goat. J. Mammary gland Biol. Neoplasia 14 223—242.

  • 23. Schedin P. Keely P. J. 2011: Mammary gland ECM remodeling stiffness and mechanosignaling in normal development and tumor progression. Cold Spring Harb. Perspect. Biol. 1 3 a003228.

  • 24. Seidal T. 2007: Immunoreactivity to desmin in secretory epithelium of eccrine sweat glands. Histopathology 18 89—91.

  • 25. Warburton M. J. Monaghan P. Ferns S. A. Hughes C. M. Rudland P. S. 1983: Distribution and synthesis of type V collagen in the rat mammary gland. J. Histochem. Cytochem. 31 1265—1273.

  • 26. Xu B. deWaal R. M. Mor-Vaknin N. Hibbard C. Markovitz D. M. Kahn M. L. 2004: The endothelial cell-specific antibody PAL-E identifies a secreted form of vimentin in the bood vasculature. Mol. Cell Biol. 24 9198—9206.

Journal information
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 170 117 2
PDF Downloads 123 86 4