Antifungal Effect of Selected Essential Oils on Malassezia pachydermatis Growth

P. Váczi 1 , E. Čonková 1 , D. Marcinčáková 1  und Z. Sihelská 1
  • 1 Institute of Pharmacology, Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, 041 81, Košice, Slovakia


The antifungal activities of 14 selected essential oils (at the concentrations of 0.5 %, 5 %, and 30 %) against the yeast Malassezia pachydermatis (18 isolates and one reference strain) were investigated. The isolates of M. pachydermatis were obtained from swabs of external ear canals of healthy dogs using sterile swabs. The determination of the efficacy was based on a modified disc diffusion method (CLSI M44-A2). The best antifungal efficacy (100 %) was shown by clove, cinnamon and oregano at the concentration of 30 %; less significant efficacy was shown at the concentration of 5 % (38 %, 33 % and 5 %, respectively). Satureja inhibited the growth of Malassezia (efficacy of 16 %) only at the concentration of 30 %. Bergamot, lavender, juniper, cedar, sage, tea-tree, grapefruit, pine, chamomile and yarrow essential oils were not able to form inhibition zones as defined in the methodology used (greater or equal to 15 mm) in all concentrations used. Therefore, according to the interpretation criterion, they were considered ineffective. In all cases, the concentration of 0.5 % was not effective against the growth of Malassezia yeasts.

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  • 1. Batra, R., Boekhout, T., Guého, E., Cabañes, F. J., Dawson, T. L., Jr., Gupta, A. K., 2005:Malassezia Baillon, emerging clinical yeasts. FEMS Yeast Res., 5, 1101—1113.

  • 2. Cabañes, F. J., Theelen, B., Castellá, G., Boekhout, T., 2007: Two new lipid-dependent Malassezia species from domestic animals. FEMS Yeast Res., 7, 1064—1076.

  • 3. Cabañes, F. J., Veja, S., Castellá, G., 2011:Malassezia cuniculi sp. nov., a novel yeast species isolated from rabbit skin. Med. Mycol., 49, 40—48.

  • 4. Cabañes, F. J., Coutinho, S. D., Puig, L., Bragulat, M. R., Castellá, G., 2016: New lipid-dependent Malassezia species from parrots. Rev. Iberoam. Micol., 33, 92—99.

  • 5. Carson, C. F., Hammer, K. A, Riley, T. V., 2006:Melaleuca alternifolia (Tea Tree) Oil: a Review of antimicrobial and other medicinal properties. Clin. Microbiol. Rev., 19, 50—62.

  • 6. Cavaleiro, C., Pinto, E., Goncalves, M. J., Salgueiro, L., 2006: Antifungal activity of Juniperus essential oils against dermatophyte, Aspergillus and Candida strains. J. Appl. Microbiol., 100, 1333—1338.

  • 7. Chiavassa, E., Tizzani, P., Peano, A., 2014:In vitro antifungal susceptibility of Malassezia pachydermatis strains isolated from dogs with chronic and acute otitis externa. Mycopathologia, 178, 315—319.

  • 8. Chuang, P. H., Lee, C. W., Chou, J. Y., Murugan, M., Shieh, B. J., Chen, H. M., 2007: Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam. Biores. Technol., 98, 232—236.

  • 9. Clinical and Laboratory Standards Institute, 2009: M44-A2. Method for antifungal disc diffusion susceptibility testing of yeast. Approved Guideline, 2nd edn., 1—23.

  • 10. Edris, A. E., 2007: Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: A review. Phytotherapy Res., 21, 308—323.

  • 11. Ferhout, H., Bohatier, J., Guillot, J., Chalchat, J. C., 1999: Antifungal activity of selected essential oils, cinnamaldehyde and carvacrol against Malassezia furfur and Candida albicans. J. Essent. Oil Res., 11, 119—129.

  • 12. Gaitanis, G., Magiatis, P., Hantschke, M., Bassukas, I. D., Velegraki, A., 2012: The Malassezia genus in skin and systemic diseases. Clin. Microbiol. Rev., 25, 106—141.

  • 13. Gaitanis, G., Velegraki, A., 2006: Verifiable single nucleotide polymorphisms of the internal transcribed spacer 2 region for the identification of 11 Malassezia species. J. Dermatol. Sci., 43, 214—217.

  • 14. Guillot, J., Bond, R., 1999:Malassezia pachydermatis: A review. Med. Mycol., 37, 295—306.

  • 15. Hammer, K. A., Carson, C. F., Riley, T. V., 2000:In vitro activities of ketoconazole, econazole, miconazole and Melaleuca alternifolia (Tea Tree) oil against Malassezia species. Antimicrob. Agents Chemother., 44, 467—469.

  • 16. Kalemba, D., Kunicka, A., 2003: Antibacterial and antifungal properties of essential oils. Cur. Med. Chem., 10, 813—829.

  • 17. Kaneko, T., Makimura K., Abe, M., Shiota, R., Nakamura, Y., Kano, R., et al., 2007: Revised culture-based system for identification of Malassezia species. J. Clin. Microbiol., 45, 3737—3742.

  • 18. Kim, J. Y., Olivry, T., Son, W. G, 2012: Characteristics of Malassezia pachydermatis isolated from dogs and antifungal effect of essential oils. J. Vet. Clin., 29, 141—147.

  • 19. Mauldin, E. A., Scott, D. W., Miller, W. H., Jr., Smith, C. A., 1997:Malassezia dermatitis in the dog — A retrospective histopathological and immunopathological study of 86 cases (1990—1995). Vet. Dermatol., 7, 191—202.

  • 20. Nardoni, S., Mugnaini, L., Pistelli, L., Leonardi, M., Sanna, V., Perrucci, S., et al., 2014: Clinical and mycological evaluation of an herbal antifungal formulation in canine Malassezia dermatitis. J. Mycol. Med., 24, 234—240.

  • 21. Pistelli, L., Mancianti, F., Bertoli, A., Cioni, P. L., Leonardi, M., Pisseri, F., et al., 2012: Antimycotic activity of some aromatic plants essential oils against canine isolates of Malassezia pachydermatis: An in vitro assay. The Open Mycol. J., 6, 17—21.

  • 22. Raut, J. S., Karuppayil, S. M., 2014: A status review on the medicinal properties of essential oils. Ind. Crops. Prod., 62, 250—264.

  • 23. Rosato, A., Vitali, C., Gallo, D., Balenzano, L., Mallamaci, R., 2008: The inhibition of Candida species by selected essential oils and their synergism with amphotericin B. Phytomed., 15, 635—638.

  • 24. Rusenova, N., Parvanov, P., 2009: Antimicrobial activities of twelve essential oils against microorganisms of veterinary importance. Trakia J. Sci., 7, 37—43.

  • 25. Váczi, P., Čonková, E., Marcinčáková, D., Sihelská, Z., 2016: Effectiveness of essential oils on Malassezia pachydermatis. Folia veterinaria, 59, 12—17.

  • 26. Verma, R. K., Chaurasia, L., Kumar, M., 2011: Antifungal activity of essential oils against selected building fungi. Indian J. Nat. Prod. Resour., 2, 448—451.

  • 27. Weiler, C. B., Kunz de Jesus, F. P., Nardi, G. H., Loreto, E. S., Santurio, J. M., Coutinho S. D. A., et al., 2013: Susceptibility variation of Malassezia pachydermatis to antifungal agents according to isolate source. Braz. J. Microbiol., 44, 174—178.


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