Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango® Super on Bovine Lymphocytes

M. Grajciarová 1  and B. Holečková 1
  • 1 Institute of Genetics, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, , Košice, Slovakia

Abstract

This study investigated the potential genotoxic effects of the fungicide Tango® Super using methods of conventional cytogenetic analysis, fluorescence in situ hybridization (FISH) and detection of DNA fragmentation in bovine lymphocytes. After exposure of two donor cell cultures to several concentrations of fungicide (0.5, 3.0 and 15.0 mg.ml-1 for conventional cytogenetic analysis; 0.5 and 3.0 mg.ml-1 for FISH) we detected the insignificant occurrence of chromosome and chromatid breakages. In both donors we observed a significant decrease in mitotic index (MI) percentage with increasing concentrations of fungicide (P < 0.01; P < 0.001), which indicated a cytotoxic effect of the preparation. Electrophoretic analysis of DNA fragmentation in lymphocytes exposed to increasing concentrations (0.5; 1.5; 3.0; 6.0 and 15.0 mg.ml-1) of this preparation showed its ability to induce formation of fragments, which is a characteristic manifestation of the last stage of apoptosis.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Bjornlund, L., Ekelund, F., Christensen, S., Jacobsen, C. S., Kroh, P. H., Johnsen, K. , 2000: Interaction between saprotrophic fungi, bacteria and protozoa on decomposing wheat roots in soil influenced by the fungicide fenpropimorph (Corbela): a field study. Soil Biol. Biochem., 32, 967-975.

  • 2. Elmore, S., 2007: Apoptosis: A review of programmed cell death. Toxicol. Pathol., 35, 495-516.

  • 3. Galdíková, M. et al., 2014: Chromosomal aberrations induced by fungicide Tango® Super in bovine peripheral lymphocytes. Priemyselná toxikológia (Industrial Toxicology) (In Slovak). Slovak Technical University, Bratislava, 33-37.

  • 4. Garaj-Vrhovac, V., Zeljezic, D., 2002: Assessment of genome damage in a population of Croatian workers employed in pesticide production by chromosomal aberration analysis, Micronucleus assay and Comet assay. J. Appl. Toxicol., 22, 249-255.

  • 5. Matušova, M., Ďurčanska, J., 2010: Evaluation of official control of pesticide residues in foodstuffs in 2009 (In Slovak). Slovenský veterinársky časopis (Slovak Veterinary Journal), 35, 186-191.

  • 6. Pistl, J., Kovalkovičová, N., Legáth, J., Mikula, I., Holovská, V., 2004: Immunotoxicology in Veterinary Medicine (In Slovak). 1st edn., University of Veterinary Medicine, Košice, 154 pp.

  • 7. Saadat, Y. R., Saeidi, N., Zununi Vahed, S., Barzegari, A., Barar, J., 2015: An update to DNA ladder assay for apoptosis detection. Bioimpacts, 5, 25-28.

  • 8. Schmidt, F., Marx-Stoelting, P., Haider, W., Heise, T., Kneuer, C., Ladwig, M. et al., 2016: Combination effects of azole fungicides in male rats in a broad dose range. Toxicology, 355-356, 54-63.

  • 9. Schwarzbacherova, V., Wnuk, M., Lewinska, A., Potocki, L., Zebrowski, J., Koziorowski, M., 2017: Evaluation of cytotoxic and genotoxic activity of fungicide formulation Tango® Super in bovine lymphocytes. Environ. Pollut., 220, 255-263.

OPEN ACCESS

Journal + Issues

Search