The Total Antibiotics test is a microbial inhibition test which has been recently introduced for the detection of antibiotics in meat. The aim of this study was to determine whether it would be suitable for the detection of coccidiostats in poultry meat. A comparison with the Premi®Test was assessed also for the suitability of the detection of coccidiostats in poultry meat. A selection of poultry meat samples of different organ parts were assessed with 14 samples from Slovakian farms that had previously been tested for coccidiostats by the Veterinary and Food Institute in Košice. In addition, another 8 samples from varied Slovakian supermarkets such as Lidl, Billa and Tesco with samples of chicken or duck meat, were tested. Each prepared sample was added to the Total Antibiotics kit tubes and incubated. The samples from all sources showed a mixture of positive and negative results for the detection of coccidiostats.
For the Premi®Test, the samples used the same extraction procedure as the Total Antibiotics, placed in Premi®Test kit tubes and incubated. The Premi®Test demonstrated a mixture of positive and negative results, as similar to the Total Antibiotics for coccidiostats in the poultry farm samples. However, the Premi Test revealed many more negative results for the supermarket sources compared to the Total Antibiotics. Therefore, based on the total number of positive results, we concluded that Total Antibiotics is more sensitive for the detection of coccidiostats in poultry meat, but depending on the source of the samples, both Total Antibiotics and Premi®Test had either similar or opposite results for the detection of coccidiostats.
If the inline PDF is not rendering correctly, you can download the PDF file here.
1. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Official Journal of the European Communities L221 8—28.
2. Commission Regulation (EU) No. 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. Official Journal of the European Communities L15 2010 1—72.
3. Council Directive 96/23/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products. Official Journal of European Union L125 1996 1—23.
4. Gondová Z. Kožárová I. Poláková Z. Maďarová M. 2014: Comparison of four microbiological inhibition tests for the screening of antimicrobial residues in the tissues of food-producing animals. Ital. J. Anim. Sci. 13 728—734.
5. Huet A. C. Bienenmann-Ploum M. Vincent U. Delahaut P. 2013: Screening methods and recent developments in the detection of anticoccidials. Anal. Bioanal. Chem. 405 7733—7751.
6. McEvoy J. D. G. 2002: Contamination of animal feeding-stuffs as a cause of residues in food: a review of regulatory aspects incidence and control. Anal. Chim. Acta 473 177−182.
7. Regulation (EC) No. 178/2002 of the European Parliament and Council of 28 January 2002 laying down the general principles and requirements of food law establishing the European Food Safety Authority and laying down procedures in matters of food safety. Official Journal of the European Union L31 2002 1—24.
8. Regulation (EC) No. 1831/2003 of the European Parliament and of the Council of 22 September 2003 on additives for use in animal nutrition. Official Journal of the European Communities L268 29—43.
9. Regulation (EC) No. 470/2009 of the European Parliament and of the Council of 6 May 2009 laying down Community procedures for the establishment of residue limits of pharmacologically active substances in foodstuffs of animal origin. Official Journal of European Union L152 2009 11—22.