The aim of the research was the analysis of 11 DH-R2 pepper (Capsicum annuum L.) lines, obtained in anther cultures of ‘(ATZ1 × PO)’ F1, ‘(ATZ1 × CDT)’ F1 and ‘(ATZ1 × TG)’ F1 hybrids. To determine the genetic homogeneity of anther-derived lines, the biometrical characteristics of fruits, as well as fruit colour and shape inheritance, were analysed. The biometrical analysis determined the highest phenotype uniformity in androgenic lines of ‘(ATZ1 × PO)’ F1. Based on the fruit shape and colour variation, it was possible to determine the microspore origin of androgenic diploids obtained in anther cultures of ‘(ATZ1 × TG)’ F1. Additionally, enzyme analysis of four isozymes (PGM, PGI, IDH and MDH) showed that isocitrate dehydrogenase identified anther-derived diploids of hybrids between ‘ATZ1’ and ‘TG’, as well as those between ‘ATZ1’ and ‘CDT’, lines.
