Implantation and pregnancy outcome of Sprague-Dawley rats exposed to pirimiphos-methyl

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Abstract

Objective. This study was designed to investigate the effect of sublethal doses (10, 60, and 120 mg/kg of pirimiphos-methyl on implantation and pregnancy in female Sprague-Dawley rats. Pirimiphos-methyl is a pesticide widely used worldwide, especially in Africa to protect food against pests and has gained widespread acceptance.

Methods. Pregnant Sprague-Dawley rats used for this study had access to food and water ad libitum and were divided into a control group and three experimental groups based on dose of chemical given. The pregnant rats were given pirimiphos-methyl orally on days 1–5, 1–7, 7–18th day of gestation and from day 1 to term. Implantation studies were carried out on days 6 and 8 of pregnancy, while the fetal parameters were ascertained on day 19 of pregnancy and at term. Serum levels of progesterone and estradiol were measured on days 6, 8 and 19 of pregnancy.

Results. Sublethal administration of pirimiphos-methyl showed decreased number of implantation sites on days 6 and 8, fetal weight, crown-to-rump length, length of umbilical cord and placenta weight (day 19), birth weight, litter size and total number (at term) in rats administered with pirimiphos-methyl when compared with control.

Conclusion. Administration of pirimiphos-methyl resulted in a reduced implantation rate due to decreased uterine receptivity caused by an imbalance in the level of estradiol and progesterone and impaired reproductive outcome during pregnancy.

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  • Baldi I Mohammed-Brahim B Brochard P Dartigues JF Salamon R. Delayed health effects of pesticides: review of current epidemiological knowledge [in French]. Rev Epidemiol Sante Publique 46 134–142 1998.

  • Berkowitz GS Obel J Deych E Lapinski R Godbold J Liu Z Landrigan PJ Wolff MS. Exposure to indoor pesticides during pregnancy in a multiethnic urban cohort. Environ Health Perspect 111 79–84 2003.

  • Barron S Foss J Riley E. The Effect of prenatal cocaine exposure on umbilical cord length in fetal rats. Neurotoxicol Teratol 13 503–506 1991.

  • Bradman A Barr DB Claus Henn BG Drumheller T Curry C Eskenazi B. Measurement of pesticides and other toxicants in amniotic fluid as a potential biomarker of prenatal exposure. Environ Health Perspect 111 1779–1782 2003.

  • Cabello G Valenzuela M Vilaxa A Duran V Rudolph I Hrepic N Calaf G. A rat mammary tumor model induced by the organophosphorous pesticides parathion and malathion possibly through acetylcholinesterase inhibition. Environ Health Perspect 109 471–479 2001.

  • Carson DD Bagchi I Dey SK Enders AC Fazleabas AT Lessey BA Koshinaga K. Embryo implantation. Dev Biol 223 217–237 2000.

  • Chahoud I Paumgartten FJR. Relationships between fetal body weight of Wistar rats at term and the extent of skeletal ossification. Braz J Med Biol Res 38 565–575 2005.

  • Dabrowski S Hanke W Polanska K Makowiec-Dabrowska T Sobala W. Pesticide exposure and birthweight: an epidemiological study in Central Poland. Int J Occup Med Environ Health 16 31–39 2003.

  • Deb K Reese J Paria BC. Methodologies to study implantation in mice. Methods Mol Med 121 9–34 2006.

  • Desforges M Sibley C. Placental nutrient supply and fetal development. Int J Dev Biol 54 377–390 2010.

  • Dey SK Lim H Das SK Reese J Paria BC Daikoku T. Wang H. Molecular cues to implantation. Endocr Rev 25 341–373 2004.

  • Eskenazi B Bradman A Castorina R. Exposure of children to organophosphate pesticides and their potential adverse health effects. Environ Health Perspect l07 409–419 1999.

  • Fenster L Coye MJ. Birthweight of infants born to Hispanic women employed in agriculture. Arch Environ Health 45 46–52 1990.

  • Furlong CE Holland N Richter RJ Bradman A Ho A Eskenazi B. PON1 status of farmworker mothers and children as a predictor of organophosphate sensitivity. Pharmacogenet Genomics 16 183–190 2006.

  • Gallo MA Lawryk NJ. Organic phosphorus pesticides. In Handbook of Pesticide Toxicology. Hayes WJJr. and Laws ER (Eds.) Academic Press New York. 1991.

  • Grether JK Harris JA Neutra R Kizer KW. Exposure to aerial malathion application and the occurrence of congenital anomalies and low birthweight. Am J Public Health 77 1009–1010 1987.

  • Guney M Oral B Demirin H Take G Giray SG Altuntas I Mungan T. Fallopian damage induced by organophosphate insecticide methyl parathion and protective effect of vitamins E and C on ultrastructural changes in rats. Toxicol Ind Health 23 428–439 2007.

  • Hamid HY Zakaria MA Yimer N Meng GY Haron AW Mustapha NM. Effects of elevated ambient temperature on embryo implantation in rats. Afri J Biotechnol 11 6624–6632 2012.

  • Hayes J Laws E. Handbook of Pesticide Toxicology. Academic Press San Diego. 1991.

  • Holland N Furlong C Bastaki M Richter R Bradman A Huen K. Paraoxonase polymorphisms haplotypes and enzyme activity in Latino mothers and newborns. Environ Health Perspect 114 985–999 2006.

  • Iranloye B Oyeusi K. Alada A. Effect of aqueous extract of Phyllanthus amarus leaf on implantation and pregnancy in rats. Niger J Physiol Sci 25 63–66 2010.

  • Iranloye BO Oyesola TO. The organophosphate - pirimiphos-methyl alters estrous cycle and hormone concentrations in female Sprague-Dawley rats. World Journal of Pharmaceutical and Life Sciences 4 207–211 2018.

  • Koch D Lu C Fisker-Andersen J Jolley L Fenske R. Temporal association of children’s pesticide exposure and agricultural spraying: report of a longitudinal biological monitoring study. Environ Health Perspect 110 829–833 2002.

  • Krakowiak P Smith EN de BG Lydon-Rochelle MT. Risk factors and outcomes associated with a short umbilical cord. Obstet Gynecol 103 119–27 2004.

  • Lesage J Del-Favero F Leonhardt M Louvart H Maccari S Vieau D Darnaudery M. Prenatal stress induces intrauterine growth restriction and programmes glucose intolerance and feeding behaviour disturbances in the aged rat. J Endocrinol 181 291–296 2004.

  • Mendoza-Rodriguez CA Merchant-Larios H Segura-Valdez ML Moreno-Mendoza N Cruz ME Arteaga-Lopez P Camacho-Arroyo I Dominguez R Cerbon M. c-fos and estrogen receptor gene expression pattern in the rat uterine epithelium during the estrous cycle. Mol Reprod Dev 64 379–388 2003.

  • Ngoula F Watcho P Dongmo MC Kenfack A Kamtchouing P Tchoumboue J. Effects of pirimiphos-methyl (an organophosphate insecticide) on the fertility of adult male rats. Afr Health Sci 7 3–9 2007.

  • Olaya C Bernal JE. Clinical associations to abnormal umbilical cord length in Latin American newborns. J Neonatal Perinatal Med 8 251–256 2015.

  • Paria BC Huet H Dey SK. Blastocyst’s state of activity determines the “window” of implantation in the receptive mouse uterus. Proc Natl Acad Sci U S A 90 10159–10162 1993.

  • Pope CN. Organophosphorous pesticides: do they all have the same mechanism of toxicity? J Toxicol Environ Health B Crit Rev 2 161–181 1999.

  • Recio R Ocampo-Gomez G Moran-Martinez J Borja-Aburto V Lopez-Cervantes M Uribe M Torres-Sanchez L Cebrian ME. Pesticide exposure alters follicle-stimulating hormone levels in Mexican agricultural workers. Environ Health Perspect 113 1160–1163 2005.

  • Sanborn MD Cole D Abelsohn A Weir E. Identifying and managing adverse environmental health effects: 4. Pesticides Can Med Assoc J 166 1431–1436 2002.

  • Tamura H Maness SC Reishman K Dorman DC Gray LE Gaido KW. Androgen receptor antagonism by the organophosphate insecticide fenilrothion. Toxicol Sci 60 56–62 2001.

  • Thomas D Petitti D Goldhaber M Swan S Rappaport E Hertz-Picciotto I. Reproductive outcomes in relation to malathion spraying in the San Francisco Bay area 1981–1982. Epidemiology 3 32–39 1992.

  • Wang H Dey SK. Roadmap to embryo implantation: clues from mouse models. Nat Rev Genet 7 185–199 2006.

  • Xiang H Nuckols JR Stallones LA. Geographic information assessment of birth weight and crop production patterns around mother’s residence. Environ Res 82 160–167 2000.

  • Zhao LH Cui XZ Yuan HJ Liang B Zheng LL Liu YX Luo MJ. Tan JH. Restraint stress inhibits mouse implantation: temporal window and the involvement of HB-EGF estrogen and progesterone. PLoS One 8 e80472 2013.

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