Expression of genes encoding IGFBPs, SNARK, CD36, and PECAM1 in the liver of mice treated with chromium disilicide and titanium nitride nanoparticles

Open access

Abstract

Objective. The aim of the present study was to examine the effect of chromium disilicide and titanium nitride nanoparticles on the expression level of genes encoding important regulatory factors (IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK/NUAK2, CD36, and PECAM1/CD31) in mouse liver for evaluation of possible toxic effects of these nanoparticles.

Methods. Male mice received 20 mg chromium disilicide nanoparticles (45 nm) and titanium nitride nanoparticles (20 nm) with food every working day for 2 months. The expression of IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK, CD36, and PECAM1 genes in mouse liver was studied by quantitative polymerase chain reaction.

Results. Treatment of mice with chromium disilicide nanoparticles led to down-regulation of the expression of IGFBP2, IGFBP5, PECAM1, and SNARK genes in the liver in comparison with control mice, with more prominent changes for SNARK gene. At the same time, the expression of IGFBP3 and CD36 genes was increased in mouse liver upon treatment with chromium disilicide nanoparticles. We have also shown that treatment with titanium nitride nanoparticles resulted in down-regulation of the expression of IGFBP2 and SNARK genes in the liver with more prominent changes for SNARK gene. At the same time, the expression of IGFBP3, IGFBP4, and CD36 genes was increased in the liver of mice treated with titanium nitride nanoparticles. Furthermore, the effect of chromium disilicide nanoparticles on IGFBP2 and CD36 genes expression was significantly stronger as compared to titanium nitride nanoparticles.

Conclusions. The results of this study demonstrate that chromium disilicide and titanium nitride nanoparticles have variable effects on the expression of IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK, CD36, and PECAM1 genes in mouse liver, which may reflect the genotoxic activities of the studied nanoparticles.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Alarifi S Ali D Alkahtani S. Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fi brosarcoma cells. Int J Nanomedicine 11 1253-1259 2016.

  • Avci NG Fan Y Dragomir A Akay YM Akay M. Investigating the infl uence of HUVECs in the formation of glioblastoma spheroids in high-throughput three-dimensional microwells. IEEE Trans Nanobioscience 14 790-796 2015.

  • Azar WJ Azar SH Higgins S Hu JF Hoffman AR Newgreen DF Werther GA Russo VC. IGFBP-2 enhances VEGF gene promoter activity and consequent promotion of angiogenesis by neuroblastoma cells. Endocrinology 12 3332-3342 2011.

  • Bach LA. Insulin-like growth factor binding proteins--an update. Pediatr Endocrinol Rev 13 521-530 2015.

  • Baxter RC. Nuclear actions of insulin-like growth factor binding protein-3. Gene 569 7-13 2015

  • Bochkov VN Philippova M Oskolkova O Kadl A Furnkranz A Karabeg E Breuss J Minchenko OH Mechtcheriakova D Hohensinner P Rychli K Wojta J Resink T Binder BR Leitinger N. Oxidized phospholipids stimulate angiogenesis via induction of VEGF IL-8 COX-2 and ADAMTS-1 metalloprotease implicating a novel role for lipid oxidation in progression and destabilization of atherosclerotic lesions. Circ Res 99 900-908 2006.

  • Bravo R Parra V Gatica D Rodriguez AE Torrealba N Paredes F Wang ZV Zorzano A Hill JA Jaimovich E Quest AF Lavandero S. Endoplasmic reticulum and the unfolded protein response: dynamics and metabolic integration. Int Rev Cell Mol Biol 301 215-290 2013.

  • Chevet E Hetz C Samali A. Endoplasmic reticulum stress-activated cell reprogramming in oncogenesis. Cancer Discov 5 586-597 2015.

  • Chu JP Yu CC Tanatsugu Y Yasuzawa M Shen YL. Non-stick syringe needles: Benefi cial eff ects of thin fi lm metallic glass coating. Sci Rep 6 31847 2016.

  • Contois LW Akalu A Caron JM Tweedie E Cretu A Henderson T Liaw L Friesel R Vary C Brooks PC. Inhibition of tumor-associated αvβ3 integrin regulates the angiogenic switch by enhancing expression of IGFBP-4 leading to reduced melanoma growth and angiogenesis in vivo. Angiogenesis 18 31-46 2015.

  • Dejeans N Barroso K Fernandez-Zapico ME Samali A Chevet E. Novel roles of the unfolded protein response in the control of tumor development and aggressiveness. Semin Cancer Biol 33 67-73 2015.

  • Ding M Bruick RK Yu Y. Secreted IGFBP5 mediates mTORC1-dependent feedback inhibition of IGF-1 signalling. Nat Cell Biol 18 319-327 2016.

  • Fu TG Wang L Li W Li JZ Li J. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37 1627-1635 2016.

  • Gokulakrishnan K Velmurugan K Ganesan S Mohan V: Circulating levels of insulin-like growth factor binding protein-1 in relation to insulin resistance type 2 diabetes mellitus and metabolic syndrome (Chennai Urban Rural Epidemiology Study 118). Metab Clin Exp 61 43-46 2012.

  • Guaita-Esteruelas S Bosquet A Saavedra P Guma J Girona J Lam EW Amillano K Borras J Masana L. Exogenous FABP4 increases breast cancer cell proliferation and activates the expression of fatty acid transport proteins. Mol Carcinog 56 208-217 2017.

  • Han J Back SH Hur J Lin YH Gildersleeve R Shan J Yuan CL Krokowski D Wang S Hatzoglou M Kilberg MS Sartor MA Kaufman RJ. ER-stress-induced transcriptional regulation increases protein synthesis leading to cell death. Nat Cell Biol 15 481-490 2013.

  • Han S Li Z Master LM Master ZW Wu A. Exogenous IGFBP-2 promotes proliferation invasion and chemoresistance to temozolomide in glioma cells via the integrin β1-ERK pathway. Br J Cancer 111 1400-1409 2014.

  • Hellewell AL Adams JC. Insider trading: Extracellular matrix proteins and their non-canonical intracellular roles. Bioessays 38 77-88 2016.

  • Hermani A Shukla A Medunjanin S Werner H Mayer D. Insulin-like growth factor binding protein-4 and -5 modulate ligand-dependent estrogen receptor-α activation in breast cancer cells in an IGF-independent manner. Cell Signal 25 1395-1402 2013.

  • Hsu HF Tsai PC Lu KC. Single-crystalline chromium silicide nanowires and their physical properties. Nanoscale Res Lett 10 50 2015.

  • Humphries B Wang Z Yang C. Th e role of microRNAs in metal carcinogen-induced cell malignant transformation and tumorigenesis. Food Chem Toxicol 98(Pt A) 58-65 2016.

  • Hwang JR Cho YJ Lee Y Park Y Han HD Ahn HJ Lee JH Lee JW. Th e C-terminus of IGFBP-5 suppresses tumor growth by inhibiting angiogenesis. Sci Rep 6 39334 2016.

  • Ilhan-Mutlu A Siehs C Berghoff AS Ricken G Widhalm G Wagner L Preusser M. Expression profi ling of angiogenesis- related genes in brain metastases of lung cancer and melanoma. Tumour Biol 37 1173-1182 2016.

  • Ingermann AR Yang YF Han J Mikami A Garza AE Mohanraj L Fan L Idowu M Ware JL Kim HS Lee DY Oh Y. Identifi cation of a novel cell death receptor mediating IGFBP-3-induced anti-tumor eff ects in breast and prostate cancer. J Biol Chem 285 30233-30246 2010.

  • Johnson MA Firth SM. IGFBP-3: a cell fate pivot in cancer and disease. Growth Horm IGF Res 24 164-173 2014.

  • Karjalainen PP Nammas W. Titanium-nitride-oxide-coated coronary stents: insights from the available evidence. Ann Med 2016.

  • Kim MS Lee DY. Insulin-like growth factor (IGF)-I and IGF binding proteins axis in diabetes mellitus. Ann Pediatr Endocrinol Metab 20 69-73 2015.

  • Kim JC Ha YJ Tak KH Roh SA Kim CW Kim TW Kim SK Kim SY Cho DH Kim YS. Complex behavior of ALDH1A1 and IGFBP1 in liver metastasis from a colorectal cancer. PLoS One 11 e0155160 2016.

  • Liu C Wu J Zou MH. Activation of AMP-activated protein kinase alleviates high-glucose-induced dysfunction of brain microvascular endothelial cell tight-junction dynamics. Free Radic Biol Med 53 1213-1221 2012.

  • Manie SN Lebeau J Chevet E. Cellular mechanisms of endoplasmic reticulum stress signaling in health and disease. 3. Orchestrating the unfolded protein response in oncogenesis: an update. Am J Physiol Cell Physiol 307 C901-C907 2014.

  • Maurel M McGrath EP Mnich K Healy S Chevet E Samali A. Controlling the unfolded protein response-mediated life and death decisions in cancer. Semin Cancer Biol 33 57-66 2015.

  • Miller HC Frampton AE Malczewska A Ottaviani S Stronach EA Flora R Kaemmerer D Schwach G Pfragner R Faiz O Kos-Kudła B Hanna GB Stebbing J Castellano L Frilling A. MicroRNAs associated with small bowel neuroendocrine tumours and their metastases. Endocr Relat Cancer 23 711-726 2016.

  • Minchenko DO Minchenko OH. SNF1/AMP-activated protein kinases: genes expression and biological role. In: Protein Kinases. Book 1 Dr. Gabriela Da Silva Xavier (Ed.). InTech 41-62 2012.

  • Minchenko DO Prylutska SV Moenner M Minchenko OH Prylutskyy YuI Schutze C Ritter U. Eff ect of C60 fullerene on the expression of ERN1 signaling related genes in human astrocytes. Mat-wiss u Werkstofft ech 44 150-155 2013a.

  • Minchenko DO Spivak MY Herasymenko RM Ivanov VK Tretyakov YD Minchenko OH. Eff ect of cerium dioxide nanoparticles on the expression of selected growth and transcription factors in human astrocytes. Mat-wiss u Werkstofft ech 44 156-160 2013b.

  • Minchenko DO Kharkova AP Karbovskyi LL Minchenko OH. Expression of insulin-like growth factor binding protein genes and its hypoxic regulation in U87 glioma cells depends on ERN1 mediated signaling pathway of endoplasmic reticulum stress. Endocr Regul 49 73-83 2015.

  • Minchenko DO Kharkova AP Tsymbal DO Karbovskyi LL Minchenko OH. Eff ect of hypoxia on the expression of genes encoding insulin-like growth factors and some related proteins in U87 glioma cells without IRE1 function. Endocr Regul 50 43-54 2016.

  • Musumeci G Castorina A Magro G Cardile V Castorina S Ribatti D. Enhanced expression of CD31/platelet endothelial cell adhesion molecule 1 (PECAM1) correlates with hypoxia inducible factor-1 alpha (HIF-1α) in human glioblastoma multiforme. Exp Cell Res 339 407-416 2015.

  • Namiki T Tanemura A Valencia JC Coelho SG Passeron T Kawaguchi M Vieira WD Ishikawa M Nishijima W Izumo T Kaneko Y Katayama I Yamaguchi Y Yin L Polley EC Liu H Kawakami Y Eishi Y Takahashi E Yokozeki H Hearing VJ. AMP kinase-related kinase NUAK2 aff ects tumor growth migration and clinical outcome of human melanoma. Proc Natl Acad Sci USA 108 6597-6602 2011.

  • Nijaguna MB Patil V Urbach S Shwetha SD Sravani K Hegde AS Chandramouli BA Arivazhagan A Marin P Santosh V Somasundaram K. Glioblastoma-derived macrophage colony-stimulating factor (MCSF) induces microglial release of insulin-like growth factor-binding protein 1 (IGFBP1) to promote angiogenesis. J Biol Chem 290 23401-23415 2015.

  • Ord T Ord D Adler P Vilo J Ord T. TRIB3 enhances cell viability during glucose deprivation in HEK293-derived cells by upregulating IGFBP2 a novel nutrient defi ciency survival factor. Biochim Biophys Acta 1853 2492-2505 2015.

  • Pascual G Avgustinova A Mejetta S Martin M Castellanos A Attolini CS Berenguer A Prats N Toll A Hueto JA Bescos C Di Croce L Benitah SA. Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature 541 41-45 2017.

  • Phillips LM Zhou X Cogdell DE Chua CY Huisinga A R Hess K Fuller GN Zhang W. Glioma progression is mediated by an addiction to aberrant IGFBP2 expression and can be blocked using anti-IGFBP2 strategies. J Pathol 239 355-364 2016.

  • Pratheeshkumar P Son YO Divya SP Wang L Turcios L Roy RV Hitron JA Kim D Dai J Asha P Zhang Z Shi X. Quercetin inhibits Cr(VI)-induced malignant cell transformation by targeting miR-21-PDCD4 signaling pathway. Oncotarget 2016.

  • Praveen Kumar VR Sehgal P Th ota B Patil S Santosh V Kondaiah P. Insulin like growth factor binding protein 4 promotes GBM progression and regulates key factors involved in EMT and invasion. J Neurooncol 116 455-464 2014.

  • Ranke MB. Insulin-like growth factor binding-protein-3 (IGFBP-3). Best Pract Res Clin Endocrinol Metab 29 701-711 2015.

  • Ritz U Nusselt T Sewing A Ziebart T Kaufmann K Baranowski A Rommens PM Hofmann A. Th e eff ect of different collagen modifi cations for titanium and titanium nitrite surfaces on functions of gingival fi broblasts. Clin Oral Investig 21 255-265 2016.

  • Rollerova E Tulinska J Liskova A Kuricova M Kovriznych J Mlynarcikova A Kiss A Scsukova S. Titanium dioxide nanoparticles: some aspects of toxicity/focus on the development. Endocr Regul 49 97-112 2015.

  • Sabin MA Russo VC Azar WJ Yau SW Kiess W Werther GA. IGFBP-2 at the interface of growth and metabolism - implications for childhood obesity. Pediatr Endocrinol Rev 8 382-393 2011.

  • Simon M Saez G Muggiolu G Lavenas M Le Trequesser Q Michelet C Deves G Barberet P Chevet E Dupuy D Delville MH Seznec H. In situ quantifi cation of diverse titanium dioxide nanoparticles unveils selective endoplasmic reticulum stress-dependent toxicity. Nanotoxicology 11 134-145 2017.

  • Sun X Gao L Chien HY Li WC Zhao J. Th e regulation and function of the NUAK family. J Mol Endocrinol 51 R15-R22 2013.

  • van Hove RP Sierevelt IN van Royen BJ Nolte PA. Titanium-nitride coating of orthopaedic implants: a review of the literature. Biomed Res Int 2015 485975 2015.

  • Wang Y Zhou XO Zhang Y Gao PJ Zhu DL. Association of the CD36 gene with impaired glucose tolerance impaired fasting glucose type-2 diabetes and lipid metabolism in essential hypertensive patients. Genet Mol Res 11 2163-2170 2012.

  • Wang J Ding N Li Y Cheng H Wang D Yang Q Deng Y Yang Y Li Y Ruan X Xie F Zhao H Fang X. Insulin-like growth factor binding protein 5 (IGFBP5) functions as a tumor suppressor in human melanoma cells. Oncotarget 6 20636-20649 2015.

  • Yang J Luo M Tan Z Dai M Xie M Lin J Hua H Ma Q Zhao J Liu A. Oral administration of nano-titanium dioxide particle disrupts hepatic metabolic functions in a mouse model. Environ Toxicol Pharmacol 49 112-118 2016.

  • Ze Y Hu R Wang X Sang X Ze X Li B Su J Wang Y Guan N Zhao X Gui S Zhu L Cheng Z Cheng J Sheng L Sun Q Wang L Hong F. Neurotoxicity and gene-expressed profi le in brain-injured mice caused by exposure to titanium dioxide nanoparticles. J Biomed Mater Res A 102 470-478 2014.

  • Zhu S Xu F Zhang J Ruan W Lai M. Insulin-like growth factor binding protein-related protein 1 and cancer. Clin Chim Acta 431 23-32 2014.

Search
Journal information
Impact Factor


CiteScore 2018: 1.27

SCImago Journal Rank (SJR) 2018: 0.411
Source Normalized Impact per Paper (SNIP) 2018: 0.441

Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 3573 2916 121
PDF Downloads 3968 3268 12