Iron (Fe) and magnesium (Mg) deficiency in human diets is a widespread problem observed in various regions of the world. Insufficient Fe uptake results in the development of iron dependent anaemia and depressed physical and intellectual performance. In turn Mg deficiency is associated with alterations in neuromuscular and cardiovascular systems. An emerging alternative to traditional supplementation of these elements in the form of pills, liquids or effervescent tablets, is introduction of fortified food products. In present study we show that preincubation of soybean seeds in Fe and Mg solutions leads to elevated content of these elements in the seedlings. Importantly the pretreatment did not affect germination rate, seedlings growth or, with an exception of Fe supplementation at highest concentration, antioxidant capacity. The obtained results indicate that preincubation of seeds in Fe and Mg solutions may be a promising method of obtaining enriched soybean sprouts.
 Dev S, Babitt JL. Overview of iron metabolism in health and disease. Hemodial Inter. 2017;21:S6-S20. DOI: 10.1111/hdi.12542.
 Benoist B, McLean E, Egli I, Cogswell M. Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia. WHO Library Cataloguing-in-Publication Data. 2008. ISBN: 9789241596657 (NLM classification: WH 155).
 Hruby A, McKeown NM. Magnesium deficiency. What is our status. Nutr Today. 2008;51:121-128. DOI: 10.1097/NT.0000000000000158.
 DiNicolantonio JJ, O’Keefe JH, Wilson W. Subclinical magnesium deficiency: a principal driver of cardiovascular disease and public health crisis. Open Heart. 2018;5:e000668. DOI: 10.1136/openhrt-2017-000668.
 Rude KR. Magnesium deficiency: A cause of heterogenous disease in humans. J Bone Mineral Res. 1998;13:749-758. DOI: 10.1359/jbmr.19220.127.116.119.
 Valavanidis A. Dietary supplements: beneficial to human health or just peace of mind? A critical review on the issue of benefit/risk of dietary supplements. Pharmakeftiki. 2016;28:69-92.
 Pachón H, Kancherla V, Handforth B, Tyler V, Bauwens L. Folic acid fortification in wheat flour: A cost-effective public health intervention to prevent birth defects in Europe. Nutr Bull. 2013;38:201-209. DOI: 10.1111/nbu.12023.
 Martorell R, de Romaña DL. Components of successful staple food fortification programs: lessons from Latin America. Food Nutr Bull. 2017;38:384-404. DOI: 10.1177/0379572117707890.
 Berner LA, Keast DR, Bailey RL, Dwyer JT. Fortified food are major contributors to nutrient intakes in diets of US children and adolescence. J Acad Nutr Diet. 2014;114:1009-1022. DOI: 10.1016/j.jand.2013.10.012.
 Bouis HE, Salzman A. Improving nutrition through biofortification: A review of evidence form HarvestPlus, 2003 through 2016. Glob Food Sec. 2017;12:49-58. DOI: 10.1016/j.gfs.2017.01.009.
 Frontasyeva MV. Neutron activation analysis in the life sciences. PEPAN. 2011;42:332-378. DOI: 10.1134/S1063779611020043.
 Pavlov SS, Dmitriev AY, Frontasyeva MV. Automation system for neutron activation analysis at the reactor IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Russia. J Radioanal Nucl Chem. 2016; 309:27-38. DOI: 10.1007/s10967-016-4864-8.
 Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol. 1995;28:25-30. DOI: 10.1016/S0023-6438(95)80008-5.
 Vasconcelos MW, Gruissem W, Bhullar NK. Iron biofortification in the 21st century: setting realistic targets, overcoming obstacles, and new strategies for healthy nutrition. Curr Opin Biotech. 2017;44:8-15. DOI: 10.1016/j.copbio.2016.10.001.
 Fischer T, Byerlee D, Edmeades G. Crop Yields and Global Food Security. ACIAR monograph no. 2014;158:xxii-634. Australian Centre for International Agricultural Research. ISBN: 9781925133066.
 Ghani M, Kulkarni KP, Song JT, Shannon JG, Lee J-D. Soybean sprouts: A review of nutrient composition, health benefits and genetic variation. Plant Breed Biotech. 2016;4:398-412. DOI: 10.9787/PBB.2016.4.4.398.
 Blicharska E, Komsta Ł, Koncjan R, Gumieniczek A, Kloc A, Kaźmierczak J. Determination of microelements in sprouts grown on metal-enriched solutions by ion chromatography. Acta Chromat. 2014;26:739-747. DOI: 10.1556/AChrom.26.2014.4.14.
 Przybysz A, Wrochna M, Małecka-Przybysz M, Gawrońska H, Gawroński SW. The effect of Mg enrichment of vegetable sprouts on Mg concentration, yield and ROS generation. J Sci Food Agric. 2016;96:3469-3476. DOI: 10.1002/jsfa.7530.
 Przybysz A, Wrochna M, Małecka-Przybysz M, Gawrońska H, Gawroński SW. Vegetable sprouts enriched with iron: effects on yields, ROS generation and antioxidant system. Scientia Horticul. 2016;203:110-117. DOI: 10.1016/j.scienta.2016.03.017.
 De Oliveira AP, Naozuka J. Effects of iron enrichment of adzuki bean (Vigna angularis) sprouts on elemental translocation, concentration of proteins, distribution of Fe-metalloproteins and Fe bioaccessibility. J Braz Chem Soc. 2017;28:1937-1946. DOI: 10.21577/0103-5053.20170034.
 Zielińska-Dawidziak M, Siger A. Effect of elevated accumulation of iron and ferritin on the antioxidants content in soybean sprouts. Eur Food Res Tech. 2012;234:1005-1012. DOI: 10.1007/s00217-012-1706-y.
 Reis S, Pavia I, Carvalho A, Moutinho-Pereira J, Correia C, Lima-Brito J. Seed priming with iron and zinc in bread wheat: effects in germination, mitosis and grain yield. Protoplasma. 2018;4:1179-1194. DOI: 10.1007/s00709-018-1222-4.
 Tie M, Sun J, Gao Y, Yao Y, Wang T, Zhong H, et al. Identification and quantification of seleno-amino acids in mung bean sprouts by high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Eur Food Res Technol. 2018;244:491-500. DOI: 10.1007/s00217-017-2967-2.