The Impact of Copper Ions on Oxidative Stress in Garden Cress Lepidium sativum

Open access

Abstract

Normal oxygen metabolism is an endogenous source of reactive oxygen species (ROS). The source of ROS are also many environmental factors including heavy metals. In certain concentration range, the presence of ROS is necessary to maintain proper cell function. Thus, cells have many mechanisms, which role is focused on maintaining a constant concentration of ROS. Imbalance between the formation of ROS and action of a protective antioxidant system leads to oxidative stress. This may results with a damage to the structure of proteins, lipids and nucleic acids, which in turn can lead to disturbances in the functioning of the cell and even to the death. The aim of the study was to evaluate the effect of copper ions on the metabolic activity of garden cress Lepidium sativum L. The action of copper ions with different concentrations was treated seeds. After four, six and eight days after planting in the leaves of garden cress were determined the specific activity of guaiacol peroxidase (GPOX), lipid peroxidation and protein content. Additionally intake of copper ions was determined using adsorption spectrometry technique. The results revealed that the applied doses of copper ions affected the activity of guaiacol peroxidase. The highest enzyme activity was found in plant material, which was treated with dose of copper ions 1000 mg/dm3 regardless of day. In the same samples the lowest level of lipid peroxidation was found. The highest concentrations of total proteins was found in samples treated with the highest dose of copper ions. The copper content in the tested plant material is correlated with the applied dose of copper ions. Our results indicate reliable correlations between copper content and values of oxidative stress biomarkers in plant tissues.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1] Pasternak T Potters G Caubergs R Jansen MAK Complementary interactions between oxidative stress and auxins control plant growth responses at plant organ and cellular level. J Exp Bot. 2005;56:1991-2001. DOI: 10.1093/jxb/eri196.

  • [2] Raldugina GN Krasavina MS Lunkova NF Burmistrova NA. Resistance of plants to Cu stress: transgenesis. In: Ahmad P editor. Plant Metal Interaction. Emerging Remediation Techniques. Elsevier. 2016:69-114. DOI: 10.1016/B978-0-12-803158-2.00004-7.

  • [3] Stohs SJ Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med. 1995;18:321-336. DOI: 10.1016/0891-5849(94)00159-H.

  • [4] Doğanlar ZB Metal accumulation and physiological responses induced by copper and cadmium in Lemna gibba L. minor and Spirodela polyrhiza. Chem Speciat Bioavialabil. 2013;15:79-88. DOI: 10.3184/095422913X13706128469701.

  • [5] Indumathy R Aruna A. Free radical scavenging activities total phenolic and flavonoid content of Lepidium sativum (Linn.). Int J Pharm Pharm Sci. 2013;5:634-637. https://www.researchgate.net/publication/288293438_Free_radical_scavenging_activities_total_phenolic_and_flavonoid_content_of_Lepidium_sativum_Linn.

  • [6] Zia-Ul-Haq M Ahmad S Calani L Mazzeo T Del Rio D Pellegrini N et al. Compositional study and antioxidant potential of Ipomoea hederacea Jacq. and Lepidium sativum L. seeds. Molecules. 2012;17:10306-10321. DOI: 10.3390/molecules170910306.

  • [7] Zaharieva T Yamashita K Matsumoto H. Iron deficiency induced changes in ascorbate contetnt and enzyme activities related to ascorbate metabolism in cucumber roots. Plant Cell Physiol. 1999;40:273-280.

  • [8] Lowry OH Rosenbrough NJ Farr AL Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-275. http://www.jbc.org/content/193/1/265.long.

  • [9] Heath RL Packer L. Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125:189-198. DOI: 10.1016/0003-9861(68)90654-1.

  • [10] Ibrahim MM Bafeel SO. Alteration of gene expression superoxide anion radical and lipid peroxidation induces by lead toxicity in leaves of Lepidium sativum. J Anim Plant Sci. 2009;4:281-288. http://www.m.elewa.org/JAPS/2009/4.1/6.pdf.

  • [11] Rajfur M Krems P Kłos A Kozłowski R Jóźwiak MA Kříž J et al. Application of algae in active biomonitoring of the selected holding reservoirs in Swietokrzyskie Province. Ecol Chem Eng S. 2016;23(2):237-247. DOI: 10.1515/eces-2016-0016.

  • [12] iCE 3000 Series AA Spectrometers Operators Manuals. Cambridge: Thermo Fisher Scientific; 2011. http://photos.labwrench.com/equipmentManuals/9291-6306.pdf.

  • [13] Lu Y Li XR He MZ Wang ZN Tan HJ. Nickel effects on growth and antioxidative enzymes activities in desert plant Zygophyllum xanthoxylon (Bunge) Maxim. Sci Cold Arid Regions. 2010;2:436-444. DOI: 10.3724/SP.J.1226.2010.00436.

  • [14] Keser G. Effects of irrigation with wastewater on the physiological properties and heavy metal content in Lepidium sativum L. and Eruca sativa (Mill.). Environ Monit Assess. 2013;185:6209-6217. DOI: 10.1007/s10661-012-3018-x.

  • [15] Upadhyay RK Panda SK. Copper-induced growth inhibition oxidative stress and ultrastructural alterations in freshly grown warer lettuce (Pistia stratiotes L.). Comptes Rendus Biol. 2009;332:623-632. DOI: 10.1016/j.crvi.2009.03.001.

  • [16] Kanoun-Boulé M Vicente JAF Nabais C Prasad MNV Freitas H. Ecophysiological tolerance of duckweeds exposed to copper. Aquat Toxicol. 2009;91:1-9. DOI: 10.1016/j.aquatox.2008.09.009.

  • [17] Srivastava S Mishra S Tripathi RD Dwivedi S Gupta DK. Copper-induced oxidative stress and responses of antioxidants and phytochelatins in Hydrilla verticillata (L.f) Royale. Aquatic Toxicol. 2006:80:405-415. DOI: 10.1016/j.aquatox.2006.10.006.

  • [18] Rolli NM Suvarnaknandi SS Mulgund GS Ratageri RH Taranath TC. Biochemical responses and accumulation of cadmium in Spirodela polyrhiza. J Environ Biol. 2010;31:529-532. http://www.jeb.co.in/journal_issues/201007_jul10/paper_23.pdf.

  • [19] Cuypers A Koistnen KM Kokko H Kärenlampi S Auriola S Vangronsveld J. Analysis of bean (Phaseolus vulgaris L.) proteins affected by copper stress. J Plant Physiol. 2005:162:383-392. DOI: 10.1016/j.jplph.2004.07.018.

  • [20] Mishra S Srivastava S Tripathi RD Kumar R Seth CS Gupta DK. Lead detoxification by Coontail (Ceratophyllum dermersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere. 2006:65:1027-1039. DOI: 10.1016/j.chemosphere.2006.03.033.

  • [21] Blokhina O Virolainen E Fagerstedt KV Antioxidants oxidative damage and oxygen deprivation stress: a review. Annal Botany. 2003;91:179-194. DOI: 10.1093/aob/mcf118.

  • [22] Passardi F Longet D Penel C Dunand C. The class III peroxidase multigenic family in rice and its evolution in land plants. Phytochemistry. 2004;65(13):1879-1893. DOI: 10.1016/j.phytochem.2004.06.023.

  • [23] Singh S Singh S Ramachandran V Eapen S. Copper tolerance and response of antioxidative enzymes in axenically grown Brassica juncea (L.) plants. Ecotoxicol Environ Safety. 2010;73:1975-1981. DOI: 10.1016/j.ecoenv.2010.08.020.

  • [24] Mourato MP Martins LL Camposa-Andrada MP. Physiological responses of Lupinus luteus to different copper concentrations. Biol Plantarium. 2009;53:105-111. https://link.springer.com/content/pdf/10.1007%2Fs10535-009-0014-2.pdf.

  • [25] Cuypers A Vangronsveld J Clijsters H. Peroxidases in roots and primary leaves of Phaseolus vulgaris copper and zinc phytotoxicity: a comparison. J Plant Physiol. 2002;159:869-876. DOI: 10.1078/0176-1617-00676.

  • [26] Karimi P Khavari-Nejad RA Niknam V Ghahremaninejad F Najafi F. The effects of excess copper on antioxidative enzymes lipid peroxidation proline chlorophyll and concentration of Mn Fe and Cu in Astragalus neo-mobayenii. Sci World J. 2012;2012:1-6. DOI: 10.1100/2012/615670.

  • [27] Meng Q Zou J Zou J Jiang W Liu D. Effect of Cu2+ concentration on growth antioxidant enzyme activity and malondialdehide content in garlic (Allium sativum L.). Acta Biol Cracoviensia Series Botan. 2007;49(1):95-101. http://www2.ib.uj.edu.pl/abc/pdf/49_1/12meng.pdf.

  • [28] Morales JML Rodriguez-Monroy M Sepúlveda-Jiménez G. Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress. Acta Soc Bot Pol. 2012;81:193-201. DOI: 10.5586/asbp.2012.019.

  • [29] Hu C Zhang L Hamilton D Zhou W Yang T Zhu D. Physiological responses induced by copper bioaccumulation in Eichhornia crassipes (Mart.). Hydrobiologia. 2007;579:211-218. DOI: 10.1007/s10750-006-0404-9.

  • [30] Monferrán MV Sánchez Agudo JA Pignata ML Wunderlin DA. Copper-induced response of physiological parameters and antioxidant enzymes in the aquatic macrophyte Potamogeton pusillus. Environ Pollut. 2009;157:2550-2576. DOI: 10.1016/j.envpol.2009.02.034.

  • [31] Fidalgo R Azenha M Silve AF de Sousan A Santiago A Ferraz P et al. Copper-induced in Solanum nigrum L. and antioxidant defense system responses. Food Energy Security. 2013;2:70-80. DOI: 10.1002/fes3.20.

  • [32] Hejazi-Mehrizi M Shariatmadari H Khoshgoftarmanesh AH Dehghani F. Copper effects on growth lipid peroxidation and total phenolic content of rosemary leaves under salinity stress. J Agr Sci Technol. 2012;14(1):205-212. https://www.researchgate.net/publication/260423986_Copper_Effects_on_Growth_Lipid_Peroxidation_and_Total_Phenolic_Content_of_Rosemary_Leaves_under_Salinity_Stress.

  • [33] Seliga H. Antioxidative activity of copper in root nodules of yellow lupin plants. Acta Physiol Plant. 1999;21:427-431.

  • [34] Szczodrowska A Kulbat K Smolińska B Leszczyńska J. Accumulation of metal ions in selected plants from Brassicaceae and Lamiaceae families. Biotechnol Food Sci. 2016;80:29-42. http://www.bfs.p.lodz.pl.

Search
Journal information
Impact Factor

IMPACT FACTOR 2018: 1.467
5-year IMPACT FACTOR: 1.226

CiteScore 2018: 1.47

SCImago Journal Rank (SJR) 2018: 0.352
Source Normalized Impact per Paper (SNIP) 2018: 0.907

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 349 165 26
PDF Downloads 204 123 18