Anti-inflammatory and analgesic activities of methanol extract of Helianthus annuus Linn. (Asteraceae) leaf

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Abstract

This study evaluate the anti-inflammatory and analgesic properties of Helianthus annuus Linn. in rats. Methanol extract of Helianthus annuus (HAE) leaf was used in this study. Formalin- and egg-albumin induced-paw edema were used to investigate the anti-inflammatory activities while acetic acid-induced writhing reflex and tail flick models were used to evaluate the analgesic properties. The doses of HAE used were 150, 300 and 600 mg/kg. Acetylsalicylic acid (ASA) was used as reference drug in the anti-inflammatory and writhing reflex models while pentazocine (reference drug) was used in tail flick model. The negative control was dosed 5% tween-20 (10 ml/kg). The HAE exhibited significant (P < 0.05) dose-dependent anti-inflammatory and analgesic activities. At 3 hour (h) post treatment, the HAE (300 mg/kg) produced 33.33% and 32.94% while ASA produced 36.36% and 35.29% reduction in paw volume in the formalin and egg-albumin induced paw edema models respectively when compared with negative control. In the acetic acid-induced writhing reflex, ASA and HAE (600 mg/kg) produced 67.89% and 35.78% reduction in the number of writhing, respectively when compared with the negative control. Pentazocine and HAE (300 mg/kg) caused 67.62% and 35.24% increase in pain reaction time when compared with the negative control. The study affirms the folkloric uses of Helianthus annuusin the management of pain and inflammation.

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  • 1. McGonagle D McDermott MF. A proposed classification of the immunological diseases. PLoS medicine 2006; 3(8): e297.

  • 2. Raicevic G Najar M Najimi M El Taghdouini A Van Grunsven LA Sokal E et al. Influence of inflammation on the immunological profile of adult-derived human liver mesenchymal stromal cells and stellate cells. Cytotherapy 2015; 17(2): 174-185.

  • 3. Heymann F Peusquens J Ludwig-Portugall I Kohlhepp M Ergen C Niemietz P et al. Liver inflammation abrogates immunological tolerance induced by Kupffer cells. Hepatology 2015; 62(1): 279-291.

  • 4. Ajayi AM Ologe MO Ben-Azu B Okhale SE Adzu B Ademowo OG. Ocimum gratissimum Linn. Leaf extract inhibits free radical generation and suppressed inflammation in carrageenan-induced inflammation models in rats. J Basic Clin Physiol Pharmacol 2017; 28(6): 531-41.

  • 5. Lee HJ Shin JS Lee KG Park SC Jang YP Nam JH et al. Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-κB and AP-1 Inactivation in Macrophages and in Endotoxic mice. Phytother Res 2017; 31(3); 475-487.

  • 6. Shahpiri Z Bahramsoltani R Farzaei MH Farzaei F Rahimi R. Phytochemicals as future drugs for Parkinson’s disease: a comprehensive review. Rev Neurosciences 2016; 27(6): 651-68.

  • 7. Onoja SO Udem SC Anaga AO. Effect of chronic administration of hydromethanol leaf extract of Helianthus annuus on Erythrocytic Profile in Normal Rats. J Nutr Ther 2017; 6(2): 59-63.

  • 8. Bashir T Zahara K Haider S Tabassum S. Chemistry pharmacology and ethnomedicinal uses of Helianthus annuus (Sunflower): A review. Pure and Applied Biology 2015; 4(2): 226-235.

  • 9. Eze VC Onoja SO Ezeja MI Eze UA. In vitro antibacterial antioxidant and phytochemical analysis of Helianthus annuus leaves extract on some bacteria causing infection. Int J Pharm Pharm Res 2015; 4(1): 93-100

  • 10. Onoja SO Anaga AO. Bioassay-guided fractionation antihyperglycemic and antioxidant properties of the methanol leaf extract of Helianthus annuus. International Journal of Pharmacognosy and Phytochemical Research 2015; 7(2): 340-346.

  • 11. Dwivedi A Sharma GN Kaushik AY. Evaluation of Helianthus annuus L. leaves extract for antidiarrheal and antihistaminic activity. Int J Res Ayurveda Pharm 2015; 6(1): 118-124.

  • 12. Ogunwande IA Flamini G Cioni PL Omikorede O Azeez RA Ayodele AA et al. Aromatic Plants growing in Nigeria: Essential Oil Constituents of Cassia alata (Linn.) Roxb. and Helianthus annuus L. Rec Nat Prod 2010; 4(4): 211-217

  • 13. Macías FA Lopez A Varela RM Molinillo JM Alves PL Torres A. Helivypolide G. A novel dimeric bioactive sesquiterpene lactone. Tetrahedron lett 2004; 45(35): 6567-6570.

  • 14. Ezeja MI Omeh YN Onoja SO Ukaonu IH. Anti-inflammatory and antioxidant activities of the methanolic leaf extract of Cissus aralioides. Am J Pharmacol Sci 2015; 3(1): 1-6.

  • 15. Onoja SO Ezeja MI Omeh YN Onwukwe BC Antioxidant anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf Alexandria J. Med. 2017; 53: 207-213.

  • 16. Evans WC. Trease and Evans Pharmacognosy 16th edition Saunders Elsevier Edinburgh 2009; 87-89.

  • 17. Onoja SO Anaga AO. Evaluation of the antidiabetic and antioxidant potentials of methanolic leaf extract of Helianthus annuus L. on alloxan-induced hyperglycemic rats. Comp Clin Patho 2014; 23: 1565-73.

  • 18. Carrillo W Spindola H Ramos M Recio I Carvalho JE. Anti-Inflammatory and Anti-Nociceptive Activities of Native and Modified Hen Egg White Lysozyme. J Med Food 2016; 19(10): 978-82.

  • 19. Krishnaraju AV Rao CB Sundararaju D Sengupta K Trimurtulu G. Anti-inflammatory activity of Vitex leucoxylon L. bark extracts against Freund’s complete adjuvant induced arthritis in Sprague Dawley rat. Am J Infect Dis 2009; 5: 68-73.

  • 20. Sofowora EA. Medicinal plants and traditional medicine in Africa. 3rd ed. Nigeria: Spectrum books Ltd. 2008.

  • 21. Vogel HG. Editor. Drug discovery and evaluation: Pharmacological assays. 2nd ed. Berlin: Springer; 2002

  • 22. Rang HP Dale MM Ritter JM Moore RK. Pharmacology. 6th Edition. Edinburgh: Elsevier Churchill Livingstone. 2009.

  • 23. Rashidian A Mehrzadi S Ghannadi AR Mahzooni P Sadr S Minaiyan M. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: a light microscopic evaluation. J Integr Med 2014; 12(2): 115-20.

  • 24. Shelmadine BD Bowden RG Moreillon JJ Cooke MB Yang P Deike E et al. A Pilot Study to Examine the Effects of an Anti-inflammatory Supplement on Eicosanoid Derivatives in Patients with Chronic Kidney Disease. J Altern Complement Med 2017; 23(8): 632-8.

  • 25. Ahmad SF Attia SM Bakheet SA Zoheir KM Ansari MA Korashy HM et al. Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines. Inflammation 2015; 38(2): 846-57.

  • 26. Yang EJ Hyun KH Kim H Kim MJ Lee NH Hyun CG. Acanthopanax koreanum roots inhibit the expression of pro-inflammatory cytokines inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages. Orient J Chem 2016; 32(1): 29-35.

  • 27. Murakami M Kudo I. Recent advances in molecular biology and physiology of the prostaglandin E2-biosynthetic pathway. Prog Lipid Res 2004; 43: 3–35.

  • 28. Wheeler-Jones CPD Farrar C Garonna E. Protease-activated receptors cyclo-oxygenases and pro-angiogenic signalling in endothelial cells. Biochem Soc Trans 2009; 37: 1179–83

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