Greenhouse study on the influence of natural biostimulators and fertilizers on improving bean plants growth and microbial activity in oil-polluted soil


The presence of oil hydrocarbons and salts in soils has as consequence the poor growth of plants due to the low availability of nutrients caused by unappropriate water regime, increased soil toxicity and the deterioration of soil structure. The present research has as main purpose the improvement of the bean plants (cultivar UNIDOR) growth and to stimulate microbial activity in rhizosphere using various natural stimulators and fertilizers (AMALGEROL,VERMIPLANT, POCO, IGUANA and FORMULEX) in greenhouse experiments on oil-polluted soil from Icoana farm, Olt county. The total counts of microorganisms (heterotrophic aerobic bacteria and filamentous fungi) were estimated by dilution plate method. The global microbial activity was measured as soil respiration by substrate-induced respiration method. Total bean plants biomass accumulation significantly increased under the influence of natural stimulators and fertilizers added (excepting IGUANA) when compared to untreated control. The best results were recorded for VERMIPLANT. Natural products induced activation of physiological activities of soil microbiota reflected in increased values of CO2 released by respiration, lower levels of colonization with phytopathogenic species, the domination of fluorescent pseudomonads, actinomycetes and cellulolytic fungi, too. The particular aspect of paper circular chromatograms reflected qualitative differences between rhizosphere soils determined by the effect of treatments with natural stimulators and fertilizers.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Nduka JK, Umeh LN, Okerulu IO, Umedum LN, Okoye HN. Utilization of different microbes in bioremediation of hydrocarbon contaminated soils stimulated with inorganic and organic fertilizers. J Pet Environ Biotechnol 2012; 3: 116. doi:

    • Crossref
    • Export Citation
  • 2. Abatenh E, Gizav B, Tsegaye Z, Wassie M. Application of microorganisms in bioremediation-review. J Environ Microbiol 2017; 1(1): 2-7.

  • 3. Bergey DH, Holt JG. Bergey’s manual of determinative bacteriology 9. Williams & Wilkins Eds., Baltimore 1994.

  • 4. Domsch KH, Gams W. Fungi in agricultural soils. T&A Constable Ltd. Edinburg, London 1970.

  • 5. Watanabe T. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species 2nd ed. 2002; CRC PRESS.

  • 6. Matei S. Determination of soil respiration and microbial biomass. In: Dumitru M, Manea A(coord.). Methods of chemical and microbiological analysis (utilized in soil monitoring system), (in Romanian). Ed. SITECH, Craiova 2011; p. 283-288.

  • 7. Matei GM, Matei S. Preliminary study of microbial communities in soil contaminated with oil hydrocarbons from Icoana. Sci Papers Agron 2017; 60(1):85-90. Ed. „Ion Ionescu de la Brad”, Iași,

  • 8. Petre S, Pele M, Draghici EM, Postamentel M. Influence of fertilizers on cucumber fruit quality. Rev Chim 2016; 67(7):1360-1362.

  • 9. Gomez Sanchez E., Solis Oba MM, Delgado Flores J, Montalvo Aguillar KH, Solis Oba A. Evaluation of the use of organic fertilizers in lettuce (Lactuca sativa) crop. J Biotechnol 2018; 280S:S82.

  • 10. Drăghici EM, Scarlat V., Pele M, Postamentel M, Somăcescu C. Usage of perlite in polluted sandy soils for potato crop. Rev Chim 2016; 67(11):12281-2286.

  • 11. Nisha P, Nayana M, Viji V. Degradation studies on diesel oil using bacterial consortium isolated from oil polluted soil. Adv Biotech 2013; 13 (2)

  • 12. Tuleva BK, Ivanov RG, Christova NE. Biosurfactant production by a new Pseudomonas putida strain. Z Naturforsch 2002; 57:356-360.

  • 13. Waogu LA, Onyeze GOC, Nwabueze RN. Degradation of diesel oil in a polluted soil in using Bacillus subtilis. Afr J Biotechnol 2008; 7(12): 1939-1943.

  • 14. Santhini K, Myla J, Sajani S, Usharani G. Screening of Micrococcus sp. from oil contaminated soil with reference to bioremediation. Bot Res Int 2009; 2(4): 248-252.

  • 15. Patowary K, Patowary R, Kalita MC, Deka S. Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Front in Microbiol 2017; 8: 279.

  • 16. Iordache O, Popa G, Dumitrescu I, Rodino S, Matei A, Cornea CP, Diguta C, Varzaru E, Ionescu I. Evaluation of decolorization abilities of some textile dyes by fungal isolates. Ind Text 2016; 67(2):181-188.

  • 17. Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Barbetti MJ, Li H, Woo SL, Lorito M. A novel role for Trichoderma secondary metabolites in the interaction with plants. Physiol Mol Pl Pathol 2008; 72: 80-86.

  • 18. Viterbo A, Landau U, Kim S, Chernin L, Chet I. Characterization of ACC deaminase from the biocontrol and plant growth-promoting agent Trichoderma asperellum T203. Fems Microbiol Lett 2010; 305: 42-48.

  • 19. Vinale F, Nigro M, Sivasithamparam K, Flematti G, Ghisalberti EL, Ruocco M, Varlese S, Marra R, Lanzuise S, Eid A, Woo SL, Lorito M. Harzianic acid: a novel siderophore from Trichoderma harzianum. Fems Microbiol Lett 2013; 347: 123-129.

  • 20. Vinale F, Strakowska J, Mazzei P, Piccolo A, Marra R, Lombardi N, Manganiello G, Pascale A, Woo SL, Lorito M. Cremenolide, a new antifungal, 10-member lactone from Trichoderma cremeum with plant growth promotion. Nat Prod Res 2016; 30: 2575-2581.

  • 21. Oancea F, Răuț I, Șesan T, Doni M, Popescu M, Zamfiropol-Cristea V, Jecu L. Trichoderma strains as plant biostimulants in high residues farming systems. In: Trichoderma spp. - applications in agriculture and horticulture, Chapter 7, Ed University, Bucharest 2017; p. 336-383.

  • 22. Kumar DS, Kumar PS, Rajendran NM, Kumar VU, Anbuganapathi G. Evaluation of Vermicompost maturity using scanning electron microscopy and paper chromatography analysis. J Agr Food Chem 2014; 62: 2738-2741.


Journal + Issues