Published Online: Jul 19, 2014
Page range: 167 - 172
Received: Nov 22, 1971
DOI: https://doi.org/10.2478/cttr-2013-0287
© Beiträge zur Tabakforschung International
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
A procedure for the automated determination of ammonia in tobacco has been developed. Ammonia is extracted from the ground tobacco sample with water and is determined with a Technicon Auto Analyser system which employs separation of the ammonia through volatilization followed by colourimetry using the phenate-hypochlorite reaction. The procedure has been applied to a variety of tobaccos containing from 0.02 to 0.5 % ammonia with an overall relative standard deviation of 2 %. The accuracy of the procedure as judged by recovery tests and by comparison to a manual distillation method is considered adequate