A simple procedure for the collection of vapor phase (VP) of mainstream cigarette smoke for analysis has been developed. This procedure consists of collecting the VP on a commercial charcoal trap (ORBO™-32) followed by dissolution in acetone. The acetone extract can be analyzed by a gas chromatography/mass spectrometry (GC/MS) technique. A qualitative analysis of the collected VP has been performed for 3R4F Kentucky reference cigarette, allowing the identification of 138 compounds, some compounds being present in both VP and in particulate phase (PP) of cigarette smoke. A quantitative analysis method for acrylonitrile and α-methacrylonitrile (2-methyl-2-propenenitrile) was also developed, and the level of these compounds in 15 different cigarette brands was measured. Acrylonitrile quantitation was selected since this compound in smoke poses significant health related issues. α-Methacrylonitrile quantitation was selected due to the similar structure of this compound with acrylonitrile. The analyzed cigarettes were several Kentucky reference cigarettes including 1R5F, 2R4F, 3R4F, 2R1F, and 1R3F, several King Size (KS) commercial cigarettes from the US market including Basic Non Filter (NF), Basic Ultra Lights (UL), Newport, Marlboro (Red), Marlboro Menthol, Camel Filter, Camel Lights, Camel Ultra Lights, and two herbal cigarettes, Ecstasy and Dreams. The results for acrylonitrile were in very good agreement with data reported in the literature for 2R4F and 1R5F cigarettes. The levels of α-methacrylonitrile were not previously reported. The correlation between the levels of acrylonitrile and of α-methacrylonitrile with the (wet) total particulate matter (TPM) was evaluated. Although the levels of acrylonitrile and of α-methacrylonitrile in mainstream smoke depend on the TPM values, the correlation is not very strong, indicating that the nature of the cigarette blend and possible other factors in cigarette construction also influence their levels in smoke. The collection method used in this study allows the subsequent dissolution of VP in a much smaller volume of solvent compared to other methods that use impingers, allows the use of standard GC/MS autosamplers for liquid injection and simple addition of internal standards compared to the methods that use gas bags, and allows a simple and immediate collection of VP as it leaves the Cambridge filter pad. These characteristics represent significant advantages versus other methods commonly used for VP analysis.
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1. Dube, M. F. and C. R. Green: Method for collection of smoke for analytical purposes; Recent Adv. Tob. Sci. 8 (1982) 42–102.
2. Byrd, G. D., K. W. Fowler, R. D. Hicks, M. E. Lovette, and M. F. Borgerding: Isotope dilution gas-chromatography-mass spectrometry in the deter-mination of benzene, toluene, styrene, and acrylonitrile in mainstream cigarette smoke; J. Chromatogr. 503 (1990) 359–368.
3. Dong, J. -Z., J. N. Glass, B. T. Thompson, B. F. Price, J. H. Lauterbach, and S. C. Moldoveanu: A simple technique for determining pH of whole cigarette smoke; Beitr. Tabakforsch. Int. 19 (2000) 33–48.
4. Diekmann, J., C. Biefel, and K. Rustemeier: Analysis of cigarette mainstream smoke for 1,1-dimethyl-hydrazine and vinyl acetate by gas chromatography-mass spectrometry; J. Chromatogr. Sci. 40 (2002) 509–514.
5. Houlgate, P. R., K. S. Dhingra, S. J. Nash, and W. H. Evans: Determination of formaldehyde and acetaldehyde in mainstream cigarette smoke by high-performance liquid chromatography; Analyst, 114 (1989) 355–360.
6. Dong, J. -Z., J. N. Glass, and S. C. Moldoveanu: A simple GC-MS technique for the analysis of vapor phase mainstream cigarette smoke; J. Microcol. Sep. 12 (2000) 142–152.
7. Omori, F., N. Higashi, M. Chida, Y. Sone, and S. Suhara: Internal standard-based analytical method for tobacco smoke vapor phase components; Beitr. Tabakforsch. Int. 18 (1999) 131–146.
8. Higgins C. E., W. H. Griest, and G. Olerich: Application of Tenax trapping to analysis of gas phase organic compounds in ultra-low tar cigarette smoke; J. Assoc. Off. Anal. Chem. 66 (1983) 1074–1083.
9. Hatzinikolaou, D. G., V. Lagesson, A. J. Stavridou, A. E. Pouli, L. Lagesson-Andrasko, and J. C. Stavrides: Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection; Anal. Chem. 78 (2006) 4509–4516.
10. Takanami, Y., M. Chida, H. Hasebe, Y. Sone, and S. Suhara: Analysis of cigarette smoke by an online thermal desorption system and multidimensional GC-MS; J. Chromatogr. Sci., 41 (2003) 317–322.
11. Koller, K. and C. Thomas: Puff-by-puff mainstream analysis by multiplex gas chromatography spectrometry; Beitr. Tabakforsch. Int. 19 (2001) 345–351.
12. Wagner K. A., R. Higby, and K. Stutt: Puff-by-puff analysis of selected mainstream smoke constituents in the Kentucky reference 2R4F cigarette; Beitr. Tabakforsch. Int. 21 (2005) 273–279.
13. Ye, Q.: Development of solid-phase microextraction followed by gas chromatography-mass spectrometry for rapid analysis of volatile organic chemicals in mainstream cigarette smoke; J. Chromatogr. A. 1213 (2008) 239–244.
14. Hoffmann, D., C. Patrianakos, K. D. Brunnemann, and G. B. Gori: Chemical studies on tobacco smoke. XXXVI. Chromatographic determination of vinyl chloride in tobacco smoke; Anal. Chem., 48 (1976) 47–50.
15. Moldoveanu, S., W. M. Coleman III, and J. Wilkins: Determination of benzene and toluene in exhaled cigarette smoke; Beitr. Tabakforsch. Int. 23 (2008) 106–113.
16. Moldoveanu, S. C. and V. David: Sample preparation in chromatography; Elsevier, Amsterdam, 2002, ISBN 0444503943.
17. EPA (1991): Integrated risk information system. US Environmental Protection Agency. Summary for acrylonitrile (CASRN 107–13–1), http://www.epa.gov/ncea/iris/subst/0206.htm (accessed October 2010).
18. IARC (1999): Re-evaluation of some organic chemicals, hydrazine and hydrogen peroxide; Lyon, International Agency for Research on Cancer (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Vol. 71).
19. Fennell, T. R., J. P. MacNeela, R. W. Morris, M. Watson, C. L. Thompson, and D. A. Bell: Hemoglobin adducts from acrylonitrile and ethylene oxide in cigarette smokers: effects of glutathione S-transferase T1-null and M1-null genotypes; Cancer Epidemiol. Biomarkers Prev. 9 (2000) 705–712.
20. Hoffmann D. and I. Hoffmann: Tobacco smoke components; Beitr. Tabakforsch. Int. 18 (1998) 49–52.
21. Nyska, A. and B. I. Ghanayem: Characterization of the toxicity, mutagenicity, and carcinogenicity of methacrylonitrile in F344 rats and B6C3F1 mice; Arch. Toxicol. 77 (2003) 233–242.
22. Pillsbury, H. C., C. C. Bright, K. J. O'Connor, and F. H. Irish: Tar and nicotine in cigarette smoke; J. Assoc. Off. Anal. Chem. 52 (1969) 458–462.
23. Intorp, M., S. Purkis, M. Whittaker, and W. Wright: Determination of “Hoffmann Analytes” in cigarette mainstream smoke. The Coresta 2006 Joint Experiment; Beitr. Tabakforsch. Int. 23 (2009) 161–202.
24. Chen, P. X. and S. C. Moldoveanu: Mainstream smoke chemical analyses for 2R4F Kentucky reference cigarette; Beitr. Tabakforsch. Int. 20 (2003) 448–458.