A Modelling Approach to Develop Machine Smoking Protocols Reflecting Human Puffing Behaviour for Conventional Cigarettes

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Our objective was to develop machine-smoking protocols (i.e. puff volume, puff duration and puff frequency) reflecting the distribution of human smoking behaviour as a complement to the International Standard (ISO) protocol. For this purpose, a modelling approach based on smoking topography and excretion data of urinary nicotine metabolites from clinical studies in the UK and Japan was used.

Three smoking protocols (LOW, MEDIUM and HIGH) were based on the 10th percentile, the mean and the 90th percentile of the puff volumes obtained from topography data. The corresponding puff durations were estimated by linear regression analysis of the puff duration/puff volume relationship. Finally, puff frequency was estimated using a predictive model assuming that the nicotine yield is a linear function (i.e. proportional) to the amount of air actually drawn through the burning part of the cigarette. This approach was termed ‘nicotine proportion method‘ where the puff frequency is changed iteratively until it equals the corresponding nicotine uptake measured in human smokers during a clinical study. The assumption was verified by measuring the nicotine yield in the laboratory on three modelled protocols for four conventional cigarettes. In 10 out of 12 cases, a very good agreement was observed between the estimated nicotine yields obtained with our modelling approach and the measured nicotine yields obtained from smoking machines in the laboratory.

Although smoking virtually identical cigarettes, smoking protocols based on topography and excretion data of urinary nicotine metabolites from the UK clinical study were different than those based on data from the study performed in Japan. This may be explained by different smoking behaviour between these two populations of smokers.

1. Ahijevych, K., H. Wee, and J. Clarke: Levels of cigarette availability and exposure in black and white women and efficient smokers; Pharmacol. Biochem. Behav. 77 (2004) 685–693.

2. Ashton, H., R. Stepney, and J.W. Thompson: Self titration by cigarette smokers; Brit. Med. J. 2 (1997) 357–360.

3. Baker, R.R.: The development and significance of standards for smoking-machine methodology; Beitr. Tabakforsch. Int. 20 (2002) 23–41.

4. Benowitz, N.L. and P. Jacob: Daily intake of nicotine during cigarette smoking; Clin. Pharmacol. Ther. 35 (1984) 499-504.

5. Benowitz, N.L. and P. Jacob: Nicotine and carbon monoxide intake from high- and low-yield cigarettes; Clin. Pharmacol. Ther. 36 (1984) 264-270.

6. Bridges, R.B., J.G. Combs, J.W. Humble, J.A. Turbek, and NJ. Haley: Puffing topography as a determinant of smoke exposure; Pharmacol. Biochem. Behav. 37 (1990) 29-39.

7. Bridges, R.B., J.W. Humble, J.A. Turbek, and S.R. Rehm: Smoking history, cigarette yield and smoking behavior as determinants of smoke exposure; Eur J Respir. Dis. 69 (1986) 129-137.

8. Byrd, G.D., R.A. Davis, W.S. Caldwell, J.H. Robinson, and J.D. deBethizy: A further study of FTC yield and nicotine absorption in smokers; Psychopharma-cology 139 (1997) 291-299.

9. Byrd, G.D., J.H. Robinson, W.S. Caldwell, and J.D. deBethizy: Comparison of measured and FTC-pre-dicted nicotine uptake in smokers; Psychopharmaco-logy 122 (1995) 95-103.

10. Clark, P.I., S. Gautam, and L.W. Gerson: Effect of menthol cigarettes on biochemical markers of smoke exposure among black and white smokers; Chest 110 (1996) 1194-1198.

11. Counts, M.E., M.J. Morton, S.W. Laffoon, R.H. Cox, and P.J. Lipowicz: Smoke composition and predicting relationships for international commercial cigarettes smoked with three machine-smoking conditions; Regul. Toxicol. Pharmacol. 41 (2005) 185-227.

12. Dwyer, R.W., P. Chen, and R. Wasyk:. A mathema-tical scheme for calculating flows and pressure drops in lit and unlit cigarettes; Beitr. Tabakforsch. Int. 19 (2001) 189-203.

13. Gori, G.B. and C.J. Lynch: Analytical cigarette yields as predictors of smoke bioavailability; Regul. Toxicol. Pharmacol. 5 (1985) 314-326.

14. Hammond, D., G.T. Fong, K.M. Cummings, and A. Hyland: Smoking topography, brand switching, and nicotine delivery: results from an in vivo study; Cancer Epidemiol. Biomarkers Prev. 14 (2005) 1370-1375.

15. Hammond, D., G.T. Fong, K.M. Cummings, R.J. O'Conner, G.A. Giovino, and A. McNeill: Cigarette yields and human exposure: A Comparison of alternative testing regimens; Cancer Epidemiol. Biomarkers Prev. 15 (2006) 1495-1501.

16. Hearn, J. and F. Yang: Evaluation of CReSSMicroTM smoking measurements devices; Communication to S. Wrenn, R. Lee and S. Yang., personal communication, Philip Morris USA, 2004.

17. International Organization for Standardization: Routine analytical cigarette-smoking machine - Defi-nitions and standard conditions; ISO 3308, 1991.

18. Jarvis, M.J., R. Boreham, P. Primatesta, C. Feyer-abend, and A. Bryant: Nicotine yield from machine-smoked cigarettes and nicotine intakes in smokers: Evidence from a representative population survey; J. Natl. Cancer Inst. 93 (2001) 134-138.

19. Kassel, J.D., J.E. Greenstein, D.P. Evatt, M.C. Wardle, M.C. Yates, J.C. Veilleux, and T. Eissenberg: Smoking topography in response to denicotinized and high-yield nicotine cigarettes in adolescent smokers; J. Adolesc. Health 40 (2007) 54–60.

20. Kolonen, S., J. Tuomisto, P. Puustinen, and M.M. Airaksinen: Smoking behavior in low-yield cigarette smokers and switchers in the natural environment; Pharmacol. Biochem. Behav. 40 (1991) 177–180.

21. Kozlowski, L.T., W.S. Rickert, M.A. Pope, J.C. Robinson, and R.C. Frecker: Estimating the yield to smokers of tar, nicotine and carbon monoxide from the lowest yield ventilated filter cigarettes; Brit. J. Addict. 77 (1982) 159–165.

22. Moody, P.M.: The relationships of quantified human smoking behavior and demographic variables; Soc. Sci. Med. 14 A (1980) 49–54.

23. Nil, R. and K. Bättig: Separate effects of cigarette smoke yield and smoke taste on smoking behavior; Psychopharmacology 99 (1989) 54–59.

24. Pérez-Stable, E., B. Herrera, P.I. Jacob, and N.L. Benowitz: Nicotine metabolism and intake in black and white smokers; J. Am. Med. Assoc. 280 (1998) 152–156.

25. Pillsbury, H.C.: Review of the Federal Trade Commission: Method for determining cigarette tar and nicotine yield; NCI Expert Committee, U.S. Department of Health and Human Services, National Institutes of Health, Monograph 7, Bethesda, MD, 1996.

26. Pillsbury, H.C., C.C. Bright, R.J. O'Conner, and F.W. Irish: Tar and nicotine in cigarette smoke; J. Assoc. Off. Anal. Chem. 52 (1969) 458–462.

27. Rickert, W.S. and J.C. Robinson: Estimating the hazards of less hazardous cigarettes, II: Study of cigarette yields of nicotine, carbon monoxide, and hydrogen cyanide in relation to levels of cotinine, carboxyhemoglobin and thiocynate in smokers; J Toxicol. Environ. Health 7 (1981) 391–403.

28. Roethig, H.J., R.D. Kinser, R.W. Lau, R.A. Walk, and N. Wang: Short-term exposure evaluation of adult smokers switching from conventional to first-generation electrically heated cigarettes during controlled smoking; J. Clin. Pharmacol. 45 (2005) 133–145.

29. Russell, M.A.H., M.J. Jarvis, R. Iyer, and C. Feyerabend: Relation of nicotine yield of cigarettes to blood concentrations in smokers; Brit. Med. J. 280 (1980) 972–976.

30. Rustemeier, K. and J.J. Piadé: Determination of nicotine in mainstream and sidestream cigarette smoke; in; Analytical determination of nicotine and related compounds and their metabolites, edited by J. W. Gorrod and P.J.I. Peyton, Elsevier, Amsterdam, 1999, pp. 489–530.

31. Strasser, A.A., R.L. Ashare, L.T. Kozlowski, and W.B. Pickworth: The effect of filter vent blocking and smoking topography on carbon monoxide levels in smokers; Pharmacol. Biochem. Behav. 82 (2005) 320–329.

32. Watson, C., J. McCraw, G. Polzin, D. Ashley, and D. Barr: Development of a method to assess cigarette smoke intake; Environ. Sci. Technol 38 (2004) 248–253.

33. Woodman, G., S.P. Newman, D. Pavia, and S.W. Clarke: Response and acclimatisation of symptomless smokers on changing to a low tar, low nicotine cigarette; Thorax 42 (1987) 336–341.

34. Woodward, M. and H. Tunstall-Pedoe: Self-titration of nicotine: Evidence from the Scottish Heart Health Study; Addiction 88 (1993) 821–830.

35. Zacny, J.P. and M.L. Stitzer: Cigarette brand-switching: Effects on smoke exposure and smoking behavior; J. Pharmacol. Exp. Ther. 246 (1988) 619–627.

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