The color of Oriental tobaccos was organoleptically assayed, and high performance liquid chromatography (HPLC) of polyphenols was performed. The major tobacco polyphenols (chlorogenic acid, its isomers, and rutin), as well as scopoletin and kaempferol-3-rutinoside were quantified. HPLC polyphenol profiles were processed by pattern recognition method (PRM), and the values of indexes of similarity (Is,%) between the cultivars studied were determined. It was shown that data from organoleptic color assessment and from PRM based on HPLC profiles of polyphenols of the cultivars studied are largely compatible. Hence, PRM can be suggested as an additional tool for objective color evaluation and classification of Oriental tobacco.
If the inline PDF is not rendering correctly, you can download the PDF file here.
1. Apostolova E. S. Dagnon and A. Edreva: Character-ization of Virginia tobaccos by chemometric methods; Beitr. Tabakforsch. Int. 20 (2002) 1–6.
2. Benavides A. I. Recasens T. Casero and J. Puy: Che-mometric analyses of “Golden Smoothee” apples treated with two preharvest calcium spray strategies in the growing season; J. Sci. Food Agr. 8 (2001) 943–952.
3. Bulgarian state standard 9271–85: Processed and ferm-ented Bulgarian Oriental tobacco; General Agency of Standardisation Sofia 1985.
4. Cantagrel R.: Application de l'analyse statistique multidimensionnelle à la différenciation des huiles essen-tielles de lavandes et de lavandins [Application of stati-stical multidimensional analysis to the differentiation of essential oils of lavenders (lavandes and lavandins)]; Parfums Cosmétiques Arômes 61 (1985) 73–76.
5. Carratala M. C. Garcia-Lopez V. Berenguer-Navarro and N. Grane-Teruel: New contribution to the che-mometric characterization of almond cultivars on the basis of their fatty acid profiles; J. Agr. Food Chem. 46 (1998) 963–967.
6. Court W.A.: High-performance reversed-phase liquid chromatography of naturally occurring phenolic com-pounds; J. Chromatogr. 130 (1977) 287–291.
7. Court W.A. M.R. Binns and J.G. Hendel: Examination of the influence of curing and stalk position on the phenolic constituents of flue-cured tobacco; Tob. Sci. 27 (1983) 51–55.
8. Court W.A. and J.G. Hendel: Phenolic constituents of flue-cured tobacco at different stages of plant growth; Tob. Sci. 29 (1985) 73–76.
9. Court W.A. J.G. Hendel and R. Poce: Fractionation of flue-cured tobacco samples using Sep-Pak car-tridges; Tob. Sci. 35 (1991) 59–62.
10. Dimov N. A. Tsoutsoulova and R. Stojanov: Pattern recognition methods for discrimination of essential oils (rose oils) by their gas chromatograms; Perfumer & Flavorist 12 (1987) 45–48.
11. Forina M. and E. Tiscorina: Pattern recognition methods in the prediction of Italian olive oils by their fatty acid content; Annali di Chimica 72 (1982) 143–146.
12. Garcia M. D. Barreiro and M. Ruiz-Altisent: Devel-opment of a virtual expert for color classification of tobacco leaves. Validation against human experts; in: Sensoral 98 – International Workshop on Sensing Qua-lity of Agricultural Products Cemagref Editions Montpellier France; Actes de Colloque Technology-Agronomy Section 1 2000 p. 3086.
13. Georgiev Ch.: Karchene i filizene na sortovete Har-manli 163 i Krumovgrad 988 [Topping and suckering of varieties Harmanli 163 and Krumovgrad 988]; Bulg. Tobacco 6 (1977) 30.
14. Grabuloski T. and Z. Taseska: Study of the correlation between the color of tobacco and the polyphenol content of Prilep tobacco; Tutun (Tobacco) 3–4 (1977) 227–291.
15. Kallianos A.G.: Phenolics and acids in leaf and their relationship to smoking quality and aroma; Rec. Adv. Tob. Sci. 2 (1976) 61–79.
16. Lulov K.: Niakoi biologichni osobenosti i agro-technicheski iziskvania na sort Nevrokop 261 [Some biological characteristics and agrotechnical reguirement for variety Nevrocop 261]; Bulg. Tobacco 4 (1972) 21-24.
17. Noble A.C.: Sensory and instrumental evaluation of wine aroma; in: Analysis of foods and beverages Academic Press Inc. N.Y. 1978 pp. 203-228.
19. Smeeton B. and M. Edwards: The influence of geno-type and curing on the polyphenol content of flue-cured tobacco polyphenols; Inf. Bull. CORESTA 3 (1993) 80.
20. Snook M.E. and O.T. Chortyk: An improved extraction-HPLC method for tobacco polyphenols; Tob. Sci. 26 (1982) 25-29.
21. Stotesbury S.J.: A method for the determination of polyphenols in tobacco by HPLC its application to the analysis of various tobacco types and to the analysis of smoke; Inf. Bull. CORESTA 3 (1993) 21.