Effect of Indigenous Pseudomonas sp. and Bacillus sp. Strains on Yield and Main Chemical Growth Parameters of Radicchio

Open access

Summary

Pseudomonas sp. and Bacillus sp. belong to plant growth promoting rhizobacteria which are able to colonize the plants roots and stimulate growth. In this study, the effect of two indigenous plant growth promoting rhizobacterial strains Pseudomonas sp. Q4 and Bacillus sp. Q10 and their mixture (mix Q4+Q10) on content of the main chemical growth parameters (nitrogen, phosphorus, potassium, calcium and magnesium) and the yield of dry biomass of radicchio (Cichorium spp. var. rossa di treviso) aerial parts and root, was investigated. The study was carried out with stagnosol type of soil in pot experiments under semi-controlled conditions in the Institute of Soil Science (Belgrade), in the period from July to October in 2013. Phosphorus was determined by spectrophotometer, potassium - by flame emission photometry and total nitrogen and carbon - using elemental CNS analyzer, while calcium and magnesium were determined by AAS. The data on yield of both aerial parts and root dry biomass of radicchio showed that its treatment with Q4 and Q10 strains, as well as with their mixture, caused noticeably increase in this parameter in relation to the control, whereby the strain Q4 was more effective for aerial parts, while mix Q4+Q10 - for roots. The obtained data on the studied chemical parameters of radicchio root and aerial parts were in total accordance with their yield. Concluding, studied strains have a potential in promoting the biomass yield and main chemical growth parameters of both aerial parts and root of radicchio.

ADESEMOYE AO, OBINI M, UGOJI EO: Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtilis in three vegetables. Braz. J. Microbiol., 39(3)423-426, 2008.

BANCHIO E, BOGINO PC, ZYGADLO J, GIORDANO W: Plant growth promoting rhizobacteria improve growth and essential oil yield in Origanum majorana L. Biochem. Syst. Ecol., 36(10)766-771, 2008.

BARCACCIA G, PALLOTTINI L, SOATTIN M, LAZZARIN R, PARRINI P, LUCCHIN M: Genomic DNA fingerprints as a tool for identifying cultivated types of radicchio (Cichorium intybus L. ) from Veneto, Italy. Plant Breed., 122:178-183, 2003.

CAKMAKCI R, DONMEZ D, AYDÝN A, SAHIN F: Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol. Biochem., 38(6)1482-1487, 2006.

CATTELAN AJ, HARTEL PG, FUHRMANN JJ: Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J., 63(6)1670-1680, 1999.

CIPRIANO M, LUPATINI M, LOPES-SANTOS L, DA SILVA M, ROESCH L, DESTÉFANO S, FREITAS S, KURAMAE E, SESSITSCH A: Lettuce and rhizosphere microbiome responses to growth promoting Pseudomonas species under field conditions. FEMS Microbiol. Ecol., 92(12):fiw197, 2016.

ĆUSTIĆ M, POLJAK M, TOTH N: Effects of nitrogen upon the quality and yield of head chicory (Cichorium intybus var. foliosum). Acta Hortic., 533:401-410, 2000.

ĆUSTIĆ M, POLJAK M, ČOGA L, ĆOSIĆ T, TOTH N, PECINA M: The influence of organic and mineral fertilization on nutrient status, nitrate accumulation, and yield of head chicory. Plant Soil Environ., 49(5)218-222, 2003.

FILIPPINI MF, NICOLETTO C, SAMBO P, CAVAGNARO JB, PIMPINI F: Influence of fertilization on the growth of radicchio “Rosso di Chioggia” cultivated in two different environments. Rev. FCA UNCUYO, 43(2)111-131, 2011.

GRAY EJ, SMITH DL: Intracellular and extracellular PGPR: Commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem., 37:395-412, 2005.

ISO 11277: 2009(E): Soil quality - determination of particle size distribution in mineral soil material - method by sieving and sedimentation. International Organization for Standardization, Geneva, Switzerland, 2009.

JAKOVLJEVIĆ M, PANTOVIĆ M, BLAGOJEVIĆ S: Praktikum iz hemije zemljišta i voda. Univerzitet u Beogradu, Poljoprivredni fakultet, 1985.

JOŠIĆ D, PROTOLIPAC K, STAROVIĆ M, STOJANOVIĆ S, PAVLOVIĆ S, MILADINOVIĆ M, RADOVIĆ S: Phenazines producing Pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. Arch. Biol. Sci., Belgrade, 64(4)1495-1503, 2012.

JOŠIĆ D, DELIĆ D, RASULIĆ N, STAJKOVIĆ O, KUZMANOVIĆ D, STANOJKOVIĆ A, PIVIĆ R: Indigenous Pseudomonads from rhizosphere of maize grown on pseudogley soil in Serbia. Bulg. J. Agric. Sci., 18:197-206, 2012a.

JOŠIĆ D, ĆIRIĆ A, SOKOVIĆ M, STANOJKOVIĆ-SEBIĆ A, PIVIĆ R, LEPŠANOVIĆ Z, GLAMOČLIJA J: Antifungal activities of indigenous plant growth promoting Pseudomonas spp. from alfalfa and clover rhizosphere. Front. Life Sci., 8(2)131-138, 2015.

LUCAS GARCÍA JA, PROBANZA A, RAMOS B, RUIZ PALOMINO M, GUTIÉRREZ MAÑERO FJ: Effect of inoculation of Bacillus licheniformis on tomato and pepper. Agronomie, EDP Sciences, 24(4)169-176, 2004.

LUGTENBERG B, KAMILOVA F: Plant growth-promoting rhizobacteria. Annu Rev. Microbiol., 63:541-556, 2009.

LUNARES F, MUÑOZ-MINGARRO D, POZUELO JM, RAMOS B, BERMÚDEZ DE CASTRO F: Microbial inhibition and nitrification potential in soils incubated with Elaeagnus angustifolia L. leaf litter. Geomicrobiol. J., 11(3-4)149-156, 1994.

MILLER R: High-temperature oxidation: dry ashing. In: Handbook of Reference Methods for Plant Analysis (Y. Kalra, ed.). CRC Press, Boca Raton, Florida, pp.53-56, 1998.

MULABAGAL V, WANG H, NGOUAJINO M, NAIR M. Characterization and quantification health of beneficial anthocyanins in leaf chicory (C. intybus) varieties. Eur. Food Res. Technol., 230:47-53, 2009.

NELSON DW, SOMMERS LE: Total carbon, organic carbon, and organic matter. In: Methods of Soil Analysis, part 3 (D.L. Sparks, ed.). SSSA, Madison, Wisconsin, pp.961-1010, 1996.

ORDOOKHANI K, SHARAFZADEH S, ZARE M: Influence of PGPR on growth, essential oil and nutrients uptake of sweet basil. Adv. Environ. Biol., 5(4)672-677, 2011.

ORHAN E, ESITKEN A, ERCISLI S, TURAN M, SAHIN F: Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci. Hort., 111(1)38-43, 2006.

PETERS AM, VAN AA: Relationship between levels of sesquiterpene lactones in chicory and sensory evaluation. J. Am. Soc. Hortic. Sci., 123:326-239, 1998

PODILE AR, KISHORE GK: Plant growth-promoting rhizobacteria. In: Plant-Associated Bacteria (S.S. Gnanamanickam, ed.). Springer, Netherlands, pp.195-230, 2006.

RANGARAJAN A, INGALL B: Mulch color affects radicchio quality and yield. Hort. Science, 36(7)1240-1243, 2001.

RIEHM H: Die Ammoniumlaktatessigsäure-Methode zur Bestimmung der leichtlöslichen Phosphorsäure in Karbonathaltigen Böden. Agrochimica, 3:49-65, 1958 (in German).

SIVASAKTHI S, USHARANI G, SARANRAJ P: Biocontrol potentiality of plant growth promoting bacteria (PGPR) - Pseudomonas fluorescens and Bacillus subtilis: a review. Afr. J. Agric. Res., 9(16)1265-1277, 2014.

SRPS ISO 10390: 2007: Soil quality - determination of pH. Institute for Standardization of Serbia, Belgrade, Serbia, 2007.

VIKRAM A, KRISHNARAJ PU, HAMZEHZARGHANI H, JAGADEESH KS: Growth promotional potential of Pseudomonas fluorescens FPD-10 and its interaction with Bradyrhizobium sp. Res. J. Microbiol., 2(4)354-361, 2007.

WRB: World Reference Base for Soil Resources - International soil classification system for naming soils and creating legends for soil maps. Food and Agriculture Organization of the United Nations, Rome, Italy, http://www.fao.org/3/a-i3794e.pdf (2014).

WRIGHT RJ, STUCZYNSKI T: Atomic absorption and flame emission spectrometry. In: Methods of Soil Analysis, part 3 (D.L. Sparks, ed.). SSSA, Madison, Wisconsin, 1996.

ZDRAVKOVIĆ J, UGRINOVIĆ M, ZDRAVKOVIĆ M, ĐORĐEVIĆ S, PAVLOVIĆ S, JOŠIĆ D: In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber. Pestic. Phytomed. (Belgrade), 30(3)169-178, 2015.

Contemporary Agriculture

The Serbian Journal of Agricultural Sciences

Journal Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 385 316 18
PDF Downloads 126 114 7