1 Timea Hajnal Jafari, PhD, Assistant Professor,, Simonida Đurić, PhD, Assistant Professor, Dragana Stamenov, PhD, Research Associate, University of Novi Sad, Faculty of Agriculture, Department for Field and Vegetable crops, Sq. D. Obradović 8, 21 000 Novi Sad, Serbia.
2 Verica Vasić, PhD, Senior Research Associate, Institute of Lowland Forestry and Enviroment, Anton Cehov 13, 21 000 Novi Sad, Serbia.
3 Davorka Hackenberger, PhD, Faculty of Education, J. J. Strossmayer University in Osijek, Department of Biology, Cara Hadrijana, Osijek, Croatia.
The aim of this research was to investigate the microbial activity in forest soil from different sites under deciduous and coniferous trees in Serbia. One site on Stara planina was under beech trees (Fagus sp.) while another under mixture of spruce (Picea sp.) and Douglas fir (Pseudotsuga sp.). The site on Kopaonik was under mixture of beech (Fagus sp.) and spruce (Picea sp.) trees. The site on Tara was dominantly under fir (Abies sp.), beech (Fagus sp.) and spruce (Picea sp.). The total number of bacteria, the number of actinobacteria, fungi and microorganisms involved in N and C cycles were determined using standard method of agar plates. The activities of dehydrogenase and ß-glucosidase enzymes were measured by spectrophotometric methods. The microbial activity was affected by tree species and sampling time. The highest dehydrogenase activity, total number of bacteria, number of actinobacteria, aminoheterotrophs, amylolytic and cellulolytic microorganisms were determined in soil under beech trees. The highest total number of fungi and number of pectinolytic microorganisms were determined in soil under spruce and Douglas fir trees. The correlation analyses proved the existence of statistically significant interdependency among investigated parameters.
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BALDRIAN, P., ŠNAJDR, J., MERHAUTOVA, V., DOBIAŠOVA, P., CAJTHAMI, T., VALAŠKOVA, V.: Responses of the extracellular enzyme activities in hardwood forest to soil temperature and seasonality and the potential effects of climate change. Soil Biology and Biochemistry, 56: 60-68, 2013.
DAS B., ASHIS CH., GHOSH S., CHAKRABARTI K.: Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology, 35: 671-678, 2011
FROSTEGARD A., BAATH E.: The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biology and Fertility of Soils, 22: 59-65, 1996.
HAYANO K.: A method for the determination of b-glukosidase activity in soil. Soil Science and Plant Nutrition, 19: 103-108, 1973.
HÖGBERG M.N., HÖGBERG P., MYROLD D.D.: Is microbial community composition in boreal forest soils determined by pH, C-to-N ratio, the trees, or all three? Oecologia, 150: 590-601, 2007.
KAISER C., FUCHSLUENGER L., KORANDA M., GORFER M., STANGE C., KITZLER B., RASCHE F., STRAUSS J., SESSITSCH A., ZECHMEISTER-BOLTENSTERN S., RICHTER A.: Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground C allocation. Ecology, 92: 1036-1051, 2011.
KANERVA S., KITUNEN V., KIIKKILÄ O., LOPONEN J., SMOLANDER A.: Response of soil C and N transformations to tannin fractions originating from Scots pine and Norway spruce needles. Soil Biology and Biochemistry, 38: 1364–1374, 2006.
KILLHAM K.: Soil ecology. Cambridge: Cambridge University Press. p. 242, 1994.
LENHARD G.: Die dehydrogenase aktivitat das Bodeusald Mass fur die Mikroorganizmentatigk eit im Boden. Z. Pflansenern. Dung Bodenkude, 73: 1-11, 1956.
LIPSON D.A., SCHMIDT S.K.: Seasonal Changes in an Alpine Soil Bacterial Community in the Colorado Rocky Mountains. Applied Environmental Microbiology, 70 (5): 2867-2879, 2004.
MANNINEN A.M., TARHANEN S., VUORINEN M., KAINULAINEN P.: Comparing the variation of needle and wood terpenoids in Scots pine provenances. Journal of Chemical Ecology, 28: 211–227, 2002.
MENYAILO O.V., HUNGATE B.A., ZECH W.: Tree species mediated soil chemical changes in a Siberian artificial afforestation experiment: tree species and soil chemistry. Plant and Soil, 242: 171-182, 2002.
MIKOLA P. : Experiment on the rate of decomposition of forest litter. Communicationes Instituti Forestalis Fenniae, 43: 1-50, 1954.
NIEMINEN T.M., SMOLANDER A.: Forest under-storey vegetation and plant litter decomposition under three different dominant tree species. In: Räty, M., Bärlund, I., Makkonen, K., Kähkönen, M. & Esala, M. (eds.). Miten maamme makaa – Suomen maaperä ja sen tila. IV Maaperätieteiden päivien laajennetut abstraktit. Pro Terra 29: 54-55, 2006.
PRIHA O., SMOLANDER A.: Microbial biomass and activity in soil and litter under Pinus sylvestris, Picea abies and Betula pendula at originally similar field afforestation sites. Biology and Fertility of Soils, 24: 45-51, 1997.
PRIHA O., GRAYSTON S.J., HIUKKA R., PENNANEN T., SMOLANDER A.: Microbial community structure and characteristics of the organic matter in soils under Pinus sylvestris, Picea abies and Betula pendula at two forest sites. Biology and Fertility of Soils, 33: 17-24, 2001.
ŠNAJDR J., VALÁŠKOVÁ V., MERHAUTOVÁ V., HERINKOVÁ J., CAJTHAML T., BALDRIAN P.: Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercus petraea forest soil. Soil Biology and Biochemistry, 40: 2068–2075, 2008.
THALMANN A.: Zur Methodik des Bestmmung des Dehydrogenase aktivitat im Bodenmittels TTC. Landiw Forch, 21: 249-258, 1968.
THEUERL S., BUSCOT F.: Laccases: Toward disentagling their diversity and functions in relation tp soil organic matter cycling. Biology and Fertility of Soils, 46: 215-225, 2010.
TROLLDENIER G.: Plate Count Technique. In: Franz Schinner, Ellen Kandeler, Richard Ohlinger, Rosa Margesin (eds.): Methods in Soil Biology…, Germany, Springer-Verlag Berlin Heideberg: 20-26, 1996.