Efficacy of Tamarind Tamarindus indica Leaves and Mango Mangifera indica Leaves as Feed Additives on Growth, Blood Status and Resistance to Aeromonas hydrophila in Juvenile African Catfish Clarias gariepinus
This study investigated the efficacy of Tamarind Leaves (TL) and Mango Leaves (ML) with Oxytetracycline (OXY) on growth performance, haematoimmunological and disease resistance of Clarias gariepinus juveniles against Aeromonas hydrophila. Experimental diets consist of control (0%), TL2 (1%), TL3 (2%), ML4 (1%), ML5 (2%), OXY6 (30mg/kg diet), (TL+ML) 7, (TL+OXY) 8, (ML+OXY) 9 and (TL+ML+OXY) 10. The fish (3.02±0.01g) were replicated twice with 20 fish per replicate and were fed twice daily at 3% body weight of 40% crude protein for twelve weeks (8 weeks for feeding trial and 4 weeks for challenge test). Mean Weight Gain (MWG), Specific Growth Rate (SGR), Packed Cell Volume (PCV), Haemoglobin (Hb), Lymphocytes (LYM), Globulin (GLO), Amino Alanine Transferase (ALT) and Aspertate Amino Transferase (AST) contents were ascertained using standard technique. The fish were infected with A. hydrophila at 5.94 log10 CFU/ml interperitoneally and fed different diets to evaluate their Relative Percent of Survival (RPS). Data was subjected to descriptive statistics and one-way analysis of variance at P=0.05. Clarias gariepinus juveniles fed treated diets had higher growth rates than the control diet but C. gariepinus fed (TL+ML+OXY) 10 had a significantly higher MWG and SGR of 7.74±0.69 g and 0.97±0.01 g, respectively. The PCV (44.0±2.00%), Hb (14.7±2.00 g/dl), LYM (37.0±2.00), GLO (42.0 ±2.00 g/dl) were higher in the C. gariepinus fed (TL+ML+OXY) 10 than the control diet. The AST and ALT values among the treated groups were lower than the values in the control at the post-challenge test. The RPS against A. hydrophila was higher in the treated groups (100%) than in the control (0%). Fish fed tamarind and mango leaves had enhanced mean weight gain and were more resistant to A. hydrophila infection.
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Abbasi, K., Anjum, N., Sammi, S., Masud, T., Ali, S. (2011): Effect of coatings and packaging material on the keeping quality of mangoes (Mangifera indica L.) stored at low temperature. Pakistan J. Nutr., 10,129-138.
AOAC (2005): Association of Official Analytical Chemist Official methods of analysis (W. Horwit zeachtor) 18th edition A.O.A.C Washington, DC, 129-146.
Bansemir, A., Blume, M., Schröder, S., Lindequist, U. (2006): Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture, 252, 79–84.
Bello, O. S., Olaifa, F. E., Emikpe, B. O. (2014): Haematological and biochemical changes in African catfish, Clarias gariepinus fed walnut (Tetracarpidium conophorum) leaf and onion (Allium cepa) bulb residues - supplemented diets. American Journal of Experimental Agriculture. 4, 12, 1593 – 1603.
Blaxhall, P. C., Daisley, K. W. (1973): Routine haematological methods for use with fish blood. Journal of Fish Biology, 5, 771.
Boukary, A. R., Chaïbou M., Marichatou H., Vias G. (2007): Caractérisation des systèmes de production laitière et analyse des stratégies de valorisation du lait en milieu rural et périurbain au Niger: cas de la communauté urbaine de Niamey et de la com-mune rurale de Filingué. Rev. Elev. Med. Vet. Pays Trop., 60, 113-120.
Das, B. K., Pradhan, J., Sahu, S. (2009): The effect of Euglena viridis on immune response of rohu, Labeo rohita (Ham.). Fish and Shellfish Immunology, 26, 871–876.
Deng, G. C, Jiang, X. Y., Ye, X., Liu, M. Z., Xu, S. Y., Liu, L. H. (2009): Isolation, identification and characterization of Aeromonas hydrophila from Hemorrhagic Grass carp. Microbiol Chin, 36, 1170-1177.
Dipali, Y, J, Akshaya K. S., Jai S. G., Rahul C. R., Aruna M. M. (2010): Phytochemical detection and in vitro evaluation of tamarind fruit pulp for potential antimicrobial activity. International Journal of Tropical Medicine, 5, 3, 68-72.
Doughari, J. H. (2006): Antimicrobial Activity of Tamarindus indica Linn. Tropical Journal of Pharmaceutical Research, 5, 2, 597-603.
Doughari, J. H., Manzara, S. (2008): In vitro antibacterial activity of crude leaf extracts of Mangifera indica Linn. Afr J Microbiol Res., 2, 067-072.
Dubber, D., Harder, T. (2008): Extracts of Ceramium rubrum, Mastocarpus stellatus and Laminaria digitata inhibit growth of marine and fish pathogenic bacteria at ecologically realistic concentrations. Aquaculture, 274, 196–200.
Eyo, A. A. (1995): Fish feed and feeding practices in Nigeria In: Report of national Aquaculture Diagnostic Survey; May – July 1994 [Edited by Ayeni, J.S.O] National Institute of Fresh water Fisheries Research, New Bussa, Niger State Nigeria,41-51.
Fafiolu, A. O., Oso, A. O., Bangbose, A. M., Omodia, R. E., Sediq, R. M. (2006): Performance of weaner rabbits fed cottonseed cake as replacement for soyabean meal. Proceedings of the 31st Annual conference of the Nigerian Society for Animal Production. 12th–15th March, 376–379.
Garba, Y., Abubakar, A. S. (2012): Haematological Response and blood chemistry of Yankasa Rams fed Graded Levels of Tamarind (Tamarindus indica) leaves. Nigerian Journals of Basic and Applied Sciences, 20, 1, 44-48.
Gupta, C., Prakash, D., Gupta, S. (2014): Studies on the antimicrobial activity of Tamarind (Tamarindus indica) and its potential as food bio-preservative. International Food Research Journal, 21, 6, 2437-2441.
Hernandez, P., Rodriguez, P. C., Delgado, R., Walczak, H. (2007): Protective Effect of Mangifera indica L. Polyphenols on Human T Lymphocytes against Activation Induced Cell Death. Pharmacological Res., 55, 167-173.
Ibrahim, M. D, Fthi, M., Mesalhy, S., Abd El-Aty, A. M. (2010): Effect of dietary supplementation of insulin and vitamin C on the growth, haematology, innate immunity and resistance of Nile tilapia (Oreochromis niloticus). Fish and Shellfish immunology, 29, 241-246.
Isaac, A. O., Olusola, S. E., Oyelese, O. A. (2014): Microbial safety and quality of smoked Clarias gariepinus Burchell, 1822 on different concentrations of preservatives during ambient storage. International Journal of Aquaculture, 4, 21, 123 – 130.
Jadhav, V. S., Khan, S. I., Girkar, M. M., Gitte, M. J. (2006): The role of immunostimulants in fish and shrimp aquaculture. Aquatic animal health, Aquaculture Asia Magazine, July – September 2006, 24 – 27.
Jokthan, G. E., Afikwu, E. V., Olugbemi, T. S. (2003): The Utilization of Fig (Ficus thonningii) and Mango (Mangifera indica) Leaves by Rabbits. Pakistan Journal of Nutrition 2, 4, 264-266.
Lalisa, W. G. (2017): Phytochemical Screening and Antioxidant Activity of selected mango (Mangifera indica). Journal of Applied Chemistry, 10, 5, 24-28.
Lehar, J., Krueger, A. S., Avery, W., Heilbut, A. M., Johansen, L. M. (2009): Synergistic drug combinations tend to improve therapeutically relevant selectivity. Nature Biotechnology, 27, 659–666.
Mamnur, M. R., Hossain, M. S., Ali, M. F. (2013): Isolation and identification of Aeromonas hydrophila from Silver Carp and its culture environment. J. Bangladesh Agril. Univ, 11, 2, 373-376.
Olusola, S. E., Olorunfemi, B. V. (2017): Bioproductive effects of Clarias gariepinus fingerlings fed guava (Psidium guajava) leaves and drumstick (Moringa oleifera) leaves extracts supplemented diet. Journal of Applied and Tropical Agriculture, 22 (2): 156-165.
Olusola, S. E., Nwokike, C. C. (2018): Effects of dietary leaves extracts of bitter (Vernonia amygdalina) and pawpaw (Carica papaya) on the growth, feed conversion efficiency and disease resistance on juveniles Clarias gariepinus, Aquaculture Research, 49, 1858–1865.
Omitoyin, B. O. (2007): Introduction to fish farming in Nigeria, Royal bird ventures. 87.
Percival, S. S., Talcott, S. T., Chin, S. T., Mallak, A. C., Lounds-Singleton, A., Pettit-Moore, J. (2006): Neoplastic Transformation of BALB/3T3 Cells and Cell Cycle of HL-60 Cells are inhibited by mango (Mangifera indica L.) juice and mango juice extracts. The Journal of Nutr., 136, 1300-1304.
Salah El-Deen, M., Rogers, W. A. (1993): Changes in total protein and transaminase activities of grass carp exposed to Diquat. Journal of Aquatic and Animal Health 5, 280-286.
Shalaby, A. M, Khattab, Y. A., Abdel-Rahman, A. M. (2006): Effects of garlic (Allium sativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilapia. Journal of Venomous Animal Toxins including Tropical Diseases 12, 2, 172 – 201.
Sharma, A., Deo, A. D., Riteshkumar, S. T., Chanu, T. I., Das, A. (2010): Effect of Withania somnifera (L. Dunal) root as a feed additive on immunological parameters and disease resistance to Aeromonas hydrophila in Labeo rohita (Hamilton) fingerlings. Fish and Shellfish Immunology, 29, 508-512.
Zhang, G., Gong, S., Yu, D., Yuan, H. (2009): Propolis and Herbed Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker, Myxocyprinus asiaticus. Fish and Shellfish Immunology, 26, 3, 467–47.
Zheng, M., Wang, X., Templaton, L. J, Samulski, D. R., Larossa, R. A., Storz, G. (2001): Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide. Applied and Environmental Microbiology, 183, 15, 4562-70.