Biochemical aspects of KB-28 compound on physically loaded study subjects

Open access


In previous studies of actoprotective activity of 5-R-thio-tetrazolo[1,5]quinazoline derivatives in normal and complicated experimental conditions, sodium 2-(tetrazolo [1,5-c]quinazoline-5-ylthio)acetate (KB-28) was found to be the leader of the experiment. The objective of the current study was to characterize the effects of KB-28 compounds on carbohydrate and lipid exchange indices under the conditions of physical load as a possible mechanism of actoprotective effect. In the course of the experiment, the indices of carbohydrate and lipid exchange in the muscle, blood and liver of animal models were determined following a 15-day physical load course. In doing so, glucose, glycogen and total lipid concentrations were assessed. The KB-28 compound was administered daily at levels determined during the course of regular physical load normalized metabolic processes in rats. The results were then compared to a control which received intraperitoneally the equivolume 0.9% sodium chloride solution. The phenomenon of actoprotection consisted in enhancing concentrations of glycogen in skeletal muscles and liver. Compared to the control figures, this increase was 28.8% and 25.0%, accordingly. Moreover, the course of KB-28 caused a statistically significant reduction (by 32.1%) of the total serum lipid concentration in the animals under physical load. The effect may be a sign of the ability of this substance to utilize active lipolysis for improvement of the skeletal muscle performance. Having analyzed the results obtained, we can draw a conclusion that influencing the biochemical processes in the study models is one of the mechanisms of the KB-28 actoprotective effect.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Gratas-Delamarche A Derbre F Vincent S Cillard J. Physical inactivity insulin resistance and the oxidative-inflammatory loop. Free Rad Res. 2014;1:93-108.

  • 2. Nikolaidis MG Kyparos A Spanou C Paschalis V Theodorou AA Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012;215(10):1615-25. https://doi.org10.1242/jeb.067470

  • 3. Rauch HG Schonbachler G Noakes TD. Neural correlates of motor vigour and motor urgency during exercise. Sports Med. 2013;43:227-241. https://doi.org10.1007/s40279-013-0025-1

  • 4. Wilmor JH Costill DI. Physiology of sport and exercise. Illinois: Human Kinetics; 2004:726

  • 5. Lukyanchuk VD Simonova IV. Actoprotectors: pharmacology and pharmacotherapy. Pharmacol Med Toxicol. 2015;2:14-26

  • 6. Oliynyk S Oh S. The Pharmacology of Actoprotectors: Practical Application for Improvement of Mental and Physical Performance. Biomols Ther. 2012;20(5):446-56. https://doi.org10.4062/biomolther.2012.20.5.446

  • 7. Kolomoets OS Nosulenko IS Voskoboynik ОY Berest GG Kovalenko SI Trzhetsinsky SD. Actoprotective activity of 6-monosubstituted 3-R-67-dihydro-2H-[124]triazino-[23-c]quinazoline-2-ones. Curr Issues Pharm Med Sci Pract. 2016;3:59-66. https://doi.org10.14739/2409-2932.2016.3.77996

  • 8. Kolomoets OS Voskoboynik ОY Nosulenko IS Kryvoshey OV Avramenko AI Berest GG. 3’-R-spiro[cycloalkyl-1(2)(hetaryl-3 (4) 6’-[124]triazino[23-c]quinazoline]-2’(7’H)-2-оnes as a perspective class of compounds with actoprotective activity. Zaporozhye Med J. 2017;19:227-32.

  • 9. Saienko A Voloshchuk N Toziuk O Kryvoviaz O Kryvoviaz S Koval V. Assessment of MT-279 compound effect on physical endurance of rats under conditions of chronic hypokinesia. Current Issues in Pharmacy and Medical Sciences. 2017;3:134-7.

  • 10. Stepanjuk GІ Toziuk OYu Kovalenko SІ Chornoіvan NG Antipenko LM Antipenko OM inventors; National Pirogov Memorial Medical University Vinnytsya assignee. 5-R-thiotetrazolo[15-c]quinazolines which increase the physical endurance. Ukraine Patent 79229. 2013 Apr 10; Bulleten 7

  • 11. Toziuk O Kryvoviaz O Kryvoviaz S Ivko T Balicka O. Effect of 5-R-Thio-Tetrazolo[15-C]Quinazoline Derivatives on the Physical Performance in Different Types of Physical Exercise. Asian J Pharm. 2017;11(3)(Suppl):532-5.

  • 12. Antypenko OM Kovalenko SI Karpenko OV. Synthesis and hydrolytic cleavage of tetrazolo[15-c]quinazolines. Synth Commun. 2016;46:551-5.

  • 13. Toziuk O. Investigation of effect of sodium 2-(tetrazolquinazolin-5-iltio)-acetate (compound KB-28) on the metabolic processes in organism during intensive exercise. ScienceRise: Pharmaceutical Science. 2016;4:60-4.

  • 14. Toziuk O Voloshchuk N Germanyuk T Kryvoviaz O Kryvoviaz S Ivko T et. al. Characteristics of 2-(tetrazol[15-c]quinazolin-5-ylthio) acetate effect on the level of adenine nucleotides in intense physical activity. International Journal of Green Pharmacy. 2017;4:276-80.

  • 15. Contarteze RV Manchado FB Gobatto CA De Mello MA. Stress biomarkers in rats submitted to swimming and treadmill running exercises. Comp Biochem Physiol A Mol Integr Physiol. 2008;151(3):415-22.

  • 16. Hubner-Wozniak I Kosmol A Blachnio D. Anaerobic capacity of upper and lower limbs muscles in combat sports contestants. J Combat Sports and Martial. 2011;2(2):91-4.

  • 17. Chamari K Padulo J. ‘Aerobic’ and ‘Anaerobic’ terms used in exercise physiology: a critical terminology reflection. Sports Med Open. 2015;1(9):1-4.

  • 18. Kits Van Heijningen AJ Kemp A. Free and fixed glycogen in rat muscle. Biochem J. 1955;59:487-91

  • 19. Selye H. 1974. Stress without Distress. Philadelphia: Lippincott.

  • 20. Baker JS McCormick MC Robergs RA. Interaction among skeletal muscle metabolic energy systems during intense exercise. J Nutr Metab. 2010;Article ID 905612:2-13.

  • 21. Eizadi M Nazem F Zarifyan A Eghdami A Khorshidi D. The Effect of Chronic Intake of L-carnitine L-tartrate on Lipid Metabolism during Aerobic Exercise. J Kerman U Med Sci. 2010;17(2):113-20

  • 22. Ji LL Zhang Y. Antioxidant and anti-inflammatory effects of exercise: role of redox signaling. Free Rad Res. 2013;48(1):3-11.

  • 23. Pingitore A Lima G Mastorci F Quinones A Iervasi G Vassalle C. Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports. Nutrition. 2015;31(7-8):916-22.

Journal information
Impact Factor

CiteScore 2018: 0.32

SCImago Journal Rank (SJR) 2018: 0.154
Source Normalized Impact per Paper (SNIP) 2018: 0.285

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 151 151 16
PDF Downloads 86 86 18