Simultaneous determination of amlodipine and telmisartan from pharmaceutical products by way of capillary electrophoresis

Adriana Modroiu 1 , Gabriel Hancu 1 , Robert Alexandru Vlad 1 , Ștefana Stăcescu 1 , Ruxandra Soare 1  and Hajnal Kelemen 1
  • 1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Tîrgu Mureş, Gh. Marinescu 38, 540139 Tîrgu Mureş, Romania

Abstract

A rapid, simple and sensitive capillary zone electrophoresis method was developed for the simultaneous determination of amlodipine besylate and telmisartan, in fixed-dose combinations, through the utilization of a UV photodiode array detector. Electrophoretic parameters such as buffer concentration and pH, system temperature, applied voltage and injection parameters, were optimized in order to improve the efficiency of the separation. The best results were obtained when employing fused silica capillary (48 cm length X 50 μm ID) and 50 mM phosphate buffer electrolyte at pH 4.50, + 25 kV applied voltage, as well as 25°C system temperature. The separation was achieved in approximately 3 minutes, with a resolution of 4.90, while the order of migration was amlodipine followed by telmisartan. The analytical performance of the method was verified with regard to linearity, precision and robustness. In addition, the limit of detection and the quantification were calculated.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Alnajjar A.O.: Validation of a capillary electrophoresis method for the simultaneous determination of amlodipine besylate and valsartan in pharmaceuticals and human plasma. J. AOAC Int., 94, 498, 2011.

  • 2. Chabukswar A.R. et al.: Simultaneous HPTLC estimation of telmisartan and amlodipine besylate in tablet dosage form. Arch. Appl. Sci. Res., 2, 94, 2010.

  • 3. Chrysant S.G.: Using fixed-dose combination therapies to achieve blood pressure goals. Clin. Drug Investig., 28, 713, 2008.

  • 4. Ebeid W. et al.: Simultaneous determination of valsartan, amlodipine besylate and hydrochlorothiazide using capillary zone electrophoresis (CZE). Pharmazie, 70, 368, 2015.

  • 5. Hefnawy M.M., Sultan M., Al-Johar H.: Development of capillary electrophoresis technique for simultaneous measurement of amlodipine and atorvastatin from their combination drug formulations. J. Liq. Chrom. Rel. Technol., 32, 2923, 2009.

  • 6. Hillaert S., van de Bossche W.: Simultaneous determination of hydrochlorothiazide and several angiotensin-II-receptor antagonists by capillary electrophoresis. J. Pharm. Biomed. Anal., 31, 329, 2003.

  • 7. Hirpara K.P. et al.: UV spectrophotometric determination for simultaneous estimation of amlodipine besylate and telmisartan in combination. J. Pharm. Sci. Bio. Res., 2, 133, 2012.

  • 8. Kondawar M.S. et al.: UV spectrophotometric estimation of amlodipine besylate and telmisartan in bulk drug and dosage form by multiwavelenght analysis. Int. J. Pharm. Tech. Res., 3, 1274, 2011.

  • 9. Rajeswari A. et al.: RP-HPLC method development and validation for simultaneous estimation of amlodipine besylate and telmisartan in tablet dosage forms. Int. J. Pharm. Res. Anal., 3, 13, 2013.

  • 10. Ram C.V.: Fixed-dose triple-combination treatments in the management of hypertension. Manag. Care, 22, 45, 2013.

  • 11. Rowan C.G. et al.: Antihypertensive treatment and blood pressure control relative to hypertension treatment guidelines. Pharmacoepidemiol. Drug. Saf., 23, 1294, 2014.

  • 12. Segura J., Ruilope L.M.: Clinical utility of fixed-combination telmisartan-amlodipine in the treatment of hypertension. Integr. Blood Press. Control., 4, 27, 2011.

  • 13. Suresh Kumar G.V., Rajendraprasad Y.: Development and validation of reversed-phase HPLC method for simultaneous estimation of telmisartan and amlodipine in tablet dosage form. Int. J. Pharm. and Pharm. Sci. 2, 128, 2010.

  • 14. Thomas A.B. et al.: Simultaneous spectrophotometric estimation of amlodipine besylate and telmisartan in tablet dosage form. Int. J. Pharm. Tech. Res., 2, 1334, 2010.

  • 15. Wan X., Ma P., Zhang X.: A promising choice in hypertension treatment: Fixed-dose combinations. Asian J. Pharm. Sci., 9, 1, 2014.

OPEN ACCESS

Journal + Issues

Search