Designing primers potentially specific to Entamoeba gingivalis genes

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Abstract

Entamoeba gingivalis normally exists in the human oral cavity, namely in the gums, and brings about some specific diseases. However, it can also trigger some more serious illnesses. Among these are infections of the genital tract, acute osteomyelitis of the mandible and pulmonary abscess. Entamoeba gingivalis identification by light microscopy is difficult, hence polymerase chain reaction (PCR) is used. The contemporary primers for PCR are complement to 18S rRNA. This article informs the reader of the process that was involved in designing new primers for three genes which were thought to be present on the Entamoeba gingivalis genome, but their sequences were unknown. The newly obtained sequences of primers have better properties for identification purposes, compared to these which are currently used.

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  • 1. Bhaijee F. Bell D.: Entamoeba gingivalis in Acute Osteomyelitis of the Mandible. Case Reports in Medicine Article ID 357301 2011.

  • 2. Brown T.A. (2002). Genomes 2nd edition. Department of Biomolecular Sciences UMIST Manchester UK. Oxford: Wiley- Liss. Chapter in a book: 311.

  • 3. Derda M. et al.: Incidence of Entamoeba gingivalis in the oral cavity of students. Journal of stomatology 64 10: 784-795 2011.

  • 4. Dominguez R. Holmes K.C.: Actin structure and function. Annual Review of Biophysics 40:169-86 2011.

  • 5. Foda A.A. El-Malky M.M.: Prevalence of genital tract infection with Entamoeba gingivalis among copper T 380A intrauterine device users in Egypt. Contraception 85(1):108-12 2012.

  • 6. Jian B. et al.: Entamoeba gingivalis pulmonary abscess - diagnosed by fine needle aspiration. Cytojournal. 30 (5):12 2008.

  • 7. Kikuta N. Yamamoto A. Goto N.: Detection and identification of Entamoeba gingivalis by specific amplification of rRNA gene. Can J Microbiol. 42(12):1248-511996.

  • 8. Que X. Reed S. L.: Cysteine Proteinases and the Pathogenesis of Amebiasis. Clin Microbiol Rev. 13(2): 196-206 2000.

  • 9. Rozen S. Skaletsky H.: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 132:365-86 2000.

  • 10. Trim R.D. et al.: Use of PCR to detect Entamoeba gingivalis in diseased gingival pockets and to demonstrate its absence in healthy gingival sites. Parasitol Res. 109:857-864 2011.

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