Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

Open access


In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS). The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Agrawal N. et al.: Determination of Paroxetine in Blood and Urine Using Micellar Liquid Chromatography with Electrochemical Detection. J. Chromatogr. Sci. 52 1217 2014.

  • 2. Belal F. et al.: Micellar HPLC and Derivative Spectrophotometric Methods for the Simultaneous Determination of Fluconazole and Tinidazole in Pharmaceuticals and Biological Fluids. J. Chromatogr. Sci. 52 298 2014.

  • 3. Carda-Broch S. et al.: Analysis of Urine Samples Containing Cardiovascular Drugs by Micellar Liquid Chromatography with Fluorimetric Detection. J. Chromatogr. Sci. 37 93 1999.

  • 4. Enga A.T.W. Henga M.Y. Ong E.S.: Evaluation of surfactant assisted pressurized liquid extraction for the determination of glycyrrhizin and ephedrine in medicinal plants. Anal. Chim. Acta 583 289 2007.

  • 5. Evans R.L. Siitonen P.H.: Determination of Caffeine and Sympathomimetic Alkaloids in Weight Loss Supplements by High- Performance Liquid Chromatography. J. Chromatogr. Sci. 46 61 2008.

  • 6. Gil-Agusti M. et al.: Use of Micellar Mobile Phases for the Chromatographic Determination of Clorazepate Diazepam and Diltiazem in Pharmaceuticals J. Chromatogr. Sci. 38 521 2000.

  • 7. Hadjmohammadi M.R. Kamel K.: Optimization of the Separation of Quinolines in Micellar Liquid Chromatography by Experimental Design and Regression Models. Chin. J. Chem. 26 2197 2008.

  • 8. Hammouda M.E.A. et al.: Simultaneous Determination of Enalapril and Hydrochlorothiazide in Pharmaceutical Preparations Using Microemulsion Liquid Chromatography. J. Chromatogr. Sci. 53 90 2015.

  • 9. Jia C.-P. Feng F.: Optimization of the Separation and Determination of Nitidine and Chelerythrine in Zanthoxylum nitidum by High- Performance Liquid Chromatography with Fluorescence Detection. J. Chromatogr. Sci. 52 164 2014.

  • 10. Martin L. et al.: Fluorescence quenching of β -carboline alkaloids in micellar media. A study to select the adequate surfactant to use in analytical techniques. Luminescence 20 152 2005.

  • 11. Rizk M.S. et al.: Development and Validation of a Stability-Indicating Micellar Liquid Chromatographic Method for the Determination of Timolol Maleate in the Presence of Its Degradation Products. doi:10.1093/chromsci/bmu075

  • 12. Ruiz-Angel M.J. et al.: Improvement of Peak Shape and Separation Performance of b-Blockers in Conventional Reversed-Phase Columns Using Solvent Modifiers. J. Chromatogr. Sci. 41 350 2003.

  • 13. Venugopal K. et al.: Development and Validation of an Ion-Pairing RP-HPLC Method for the Estimation of Gatifloxacin in Bulk and Formulations. J. Chromatogr. Sci. 45 220 2007

Journal information
Impact Factor

CiteScore 2018: 0.32

SCImago Journal Rank (SJR) 2018: 0.154
Source Normalized Impact per Paper (SNIP) 2018: 0.285

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 400 343 5
PDF Downloads 342 326 5