Histomorphology of Salivary Glands after Ligature and Reconstruction of Common Carotid Artery in Rabbits

Open access

Histomorphology of Salivary Glands after Ligature and Reconstruction of Common Carotid Artery in Rabbits

Introduction. In occlusive diseases of carotid arteries may be involved salivary glands which play important role in oral and general homeostasis.

Aim of the study. The purpose of this study was histomorphological evaluation of rabbit's salivary glands after regional reduction af arterial circulation and reconstruction of ligated common carotid arteries comparing with normal histological sight.

Materials and methods. The experiments were authorized by the Animal Ethics Committee of the Latvian Food and Veterinary Service. 20 male Californian rabbits weighing approximately 3 kg were used. Under intravenous general anesthesia and local anesthesia the ligature of a. carotis communis dextra was done. After 28 days 10 rabbits were sacrificed and samples were taken from all major salivary glands and histological measured acinar dimensions and acinar epithelial cell.

For rest 10 rabbits under the general anesthesia were performed revascularization of a. carotis communis dextra. After 28 days the rabbits were sacrificed with intrapulmonal injection of T61. The acini dimensions and acinar epithelial cell diameter in microns in all glands were measured except buccal glands were measured.

Results. In ischemic conditions all glands react with decrease of acinar epithelial cell diameter and also diametr of functional unit of salivary gland. After revascularisation reestablishment of acini and secretory epithelial cells was observed.

Conclusions. Rabbit's salivary glands have potential to recovery in diminished after ligation of ipsilateral common carotid artery size of acini and secretory epithelium after 4 week ischemia if the blood supply is reestabilished.

References
  • Aydin Ö, Yilmaz T, Özer F, Saraç S, Sökmensüer C., Necrotizing sialometaplasia of parotid gland: a possibility vasculitic cause // Int J Pediatric Otorhinolaryn, 2002; 64:171-174

  • Azevedo LR, Damante JH, Lara VS, Lauris JRP., Age - related changes in human sublingual glands; a post mortem study // Arch Oral Biol, 2005; 50:565-574

  • Bodner L, Baum BJ., Submandibular gland secretory function in young adult and aged rats // Comparat Biochem Physiol A: Physiol, 1984; 77:235-238

  • Brannon RB, Fowler CB, Hartman KS., Necrotizing sialometaplasia: a clinicopathologic study of sixtynine cases and review of the literature // Oral Surg Oral Med Oral Pathol, 1991; 72:317-325

  • Burgess LK, Dardick I., Cell population changes during atrophy and regeneration of rat parotid gland // Oral Surg Oral Med Oral Patho Oral Radiol Endodont, 1998; 85:699-706

  • Dayan D, Bodner L, Horowitz I., Effect of salivary gland hypofunction on the healing of extraction wounds: A histomorphometric study in rats // J Oral Maxillofac Surg, 1992; 50:354-358

  • Drummond JR, Chisholm DM. A qualitative and quantitative study of the aging human labial salivary glands // Arch Oral Biol, 1984; 29:151-155

  • Fox RI, Robinson CA, Curd JG, Kozin F, Howell FV., Sjögren's syndrome. Proposed criteria for classification // Arthritis Rheum, 1986; 29:577-585

  • Fujisava Y, Aiyama S., Histochemical and chronological analysis of mouse submandibular gland parenchyma subjected to abrupt reperfusion // Histol Histopathol, 2003; 18:871-878

  • Gaubenstock LM., Dental caries and the secretory activity of human labial minor salivary glands // Arch Oral Biol, 1995; 40:525-528

  • Greenspan JS, Daniels TE, Talal N, Sylvester AR., The histopathology of Sjögren's syndrome in labial salivary gland biopsies // Oral Surg, 1974; 37:217-229

  • Hashimoto T., Histopathological changes of rat palatine glands by ligation of palatine vesels // Meikai Univ. Dent. J. 1998; 27:33-41

  • Komesu MC, Lopes AR, Petenusci SO., Silva-Netto CR, Lopes OVP, Campos GM, Alteraçöes morfologicas da glândula submandibular do rato determinadas pelo envelhecimento // Rev Fac Odontol Ribeiräo Preto, 1986; 23:79-88

  • Liu P, Denny PA, Denny P. The effect of ageing on parenchymal cell populations in adult female mouse submandibular gland // Arch Oral Biol, 2000; 45:585-592

  • Magoshi S., Histomorphological studies on the effects of ligation of the excretory duct and the main blood vessel on the rat submandibular gland: experimental development of necrotizing sialometaplasia // Meikai Univ. Dent. J. 1998; 27:20-32

  • Moreiraa Carla Ruffeil, Azevedob Luciana Reis, Laurisd JRP, Tagae R, Damantec JH., Quantitative age-related differences in human sublingual gland // Arch Oral Biol, 2006; 51:960-966

  • Ohsava K, Ushida M, Aiyama S., Three-dimensional observation of blood vesels supplying the gland proper and capsule of the mouse submandibular gland // Ann Anat, 2006; 188:61-67

  • Scott J., Degenerative changes in the histology of the human submandibular salivary gland occuring with age // J Biol Buccales, 1977; 5:311-319

  • Scott J., Qualitative and quantitative observations on the histology of human labial salivary glands obtained post mortem // J Biol Buccale, 1980; 8:187-200

  • Scott J., Quantitative age changes in the histological structure of human submandibular salivary glands // Arch Oral Biol, 1977; 22:221-225

  • Scott J., The incidence of focal chronic inflammatory changes in human submandibular salivary glands // J Oral Pathol, 1976; 5:334-346

  • Standish SM, Shafer WG., Serial histologic effects of rat submaxillary and sublingual salivary gland duct and blood vessel ligation // J Dent Res, 1957; 36:866-879

  • Takahashi Y, Kurabuchi S, Aiyama S., Histological changes in the mouse submandibular gland subjected to parasympathetic nerve block or ischemia: comparison between chorda tympani resection and trophic vessel transection // SHIGAKU (Odontology) 1999; 86:826-841

  • Vered M, Buchner A, Haimovici E, Hiss Y, Dayan D., Focal lymphocytic infiltration in aging human palatal salivary glands: a comparative study with labial salivary glands // J Oral Pathol Med, 2001; 30:710-716

  • Waterhouse JP, Chisholm DM, Winter RB, Paterand M, Yale RS., Replace-ment of functional parenchymal cells by fat and connective tissue in human submandibular salivary glands: age related changes // J Oral Pathol, 1973; 14:16-27

  • Wilson DF., Histology of the salivary glands // In Textbook of Oral and Maxillofacial Anatomy, Histology and Embryology edited by S. R. Prabhu, Oxford: 2006;169-179

Acta Chirurgica Latviensis

The Journal of Riga Stradins University; Latvian Association of Surgeons; Latvian Association of Paediatric Surgeons

Journal Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 10 10 10
PDF Downloads 1 1 1