Osmo and Hydro Priming Mediated Germination Improvement Under Cold Stress Conditions in Mountain Rye (Secale Montanum)

O. Ansari 1  and F. Sharif Zadeh 2
  • 1 Agronomy and Faculty of Natural Campus, University of Tehran, Karaj, Iran
  • 2 College of Agriculture and Natural Resources, University of Tehran, Iran


Seed priming techniques have been used to increase germination, improve germination uniformity and seedling establishment under stressed conditions. Seed priming was used in rye mountain (Secale montanum) to increase seed germination and tolerance on stress exposure (Cold stress). Rye seeds were treated with water (Hydro priming) and polyetelyn glycol 6000 (Osmo priming). The effect of priming was assessed on germination characteristics under cold stress (at 3°C) for 7 days. Analyze of variance for hydro priming showed that temperature × time of priming interaction was significantly (P < 0.01) for all traits under cold stress and for osmo priming showed that Concentration of PEG × Temperature × Time of priming interaction was significantly (P < 0.01) for all traits under cold stress except seedling dry weight (SDW). For hydro priming the highest germination percentage (GP) was attained from hydro priming at 15±1°C for 16 h (70%), whereas the highest normal seedling percentage (NSP) (22.5%), germination index (GI) (8.57), seedling vigor index (SVI) (38.9) and seedling length (SL) (1.7 cm) were in the hydro priming at 10±1°C for 16 h. For osmo priming the highest germination percentage (GP) (80 and 80.5%), normal seedling percentage (NSP) (48 and 45%), seedling vigor index (SVI) (101.26 and 85.25) and seedling length (SL) (2.11 and 1.89 cm) observed in the osmo priming with concentrations of -9 and -15 bar PEG at 15±1°C for 24 h.

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  • Almansouri M., Kinet JM., Lutts S., 2001- Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil 231: 243-254.

  • Ashraf M., Foolad M.R., 2005 - Presowing seed treatment-a shotgun approach to improve germination growth and crop yield under saline and none-saline conditions, Advan. Agron 88: 223-271.

  • Bradford K.J., 1986 - Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hurt. Sci 21: 1105-1112.

  • Casenave E.C., Toselli M.E., 2007 - Hydro priming as a pre-treatment for cotton germination under thermal and water stress conditions. Seed Sci: Technol 35: 88-98.

  • Chen D., Gunawardena T.A., Naidub P.,Fukai S., Basnaykae J., 2005 - Seed treatment with gibberellic acid and glycinebetaine improves seedling emergence and seedling vigour of rice under low temperature. Seed Science and Technology 33: 471-479.

  • De Bustos A., Jouve N., 2002 - Phylogenetic relationships of the genus Secale based on the characterization of rDNA ITS sequences. Plant Syst. Evol 235: 147-154.

  • El-Tayeb M.A., 2005 - Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regul 45: 215-224.

  • Fu J.R., Lu X.H., Chen R.Z., Zhang B.Z.,Liu Z.S., Li Z.S., Cai D.Y., 1988 - Osmoconditioning of peanut Arachishypogaea L. seeds with PEG to improve vigour and some biochemical activities, Seed Sci. Technol 16: 197-212.

  • Ganji Arjenaki F., Amini Dehaghi M.,Jabbari R., 2011 - Effects of Priming on Seed Germination of Marigold (Calendula officinalis). Advances in Environmental Biology 5(2): 276-280.

  • Gao Y.P., Young L., Bonham-smith P.,Gusta L.V., 1999 - Characterization and expression of plasma and tonoplast membrane aquaporins in primed seed of Brassica napus during germination under stress conditions, Plant Mol. Biol 40: 635-444.

  • Gharib F.A., Hegazi A.Z., 2010 - Salicylic acid ameliorates germination, seedling growth, phytohormone and enzymes activity in bean (Phaseolusvulgaris L.) under cold stress. Journal of American Science 6(10): 675-683.

  • Ghassemi-Golezani K., Esmaeilpour B.,2008 - The effect of salt priming on the performance of differentially motured cucumber (Cucumissativus) seeds. Not. Bot. Hort Agrobot. Cluj- Napoca 36: 67-70.

  • Hamad A., Hamada A., 2001 - Grain soaking presowing in ascorbic acid or thiamin versus the adverse effects of combined salinity and drought on wheat seedlings. in Proceedings of the 12th International Congress on Photosynthesis (Melbourne, Australia, Brisbane, Australia, August 18-23, Augustm 18-23, 2001), CSIRO Publishing S15-005.

  • Hardegree S.P., Van Vactor S.S., 2000 - Germination and emergence of primed grass seeds under field and simulatedfield temperature regimes. Annals of Botany 85: 379-390.

  • Hayat S., Fariduddin Q., Ali B., AhmedA., 2005 - Effect of salicylic acid on growth and enzyme activities of wheat seedlings. Acta Agron Hung 53: 433-437.

  • Iqbal M., Ashraf M., 2007 - Seed treatment with auxins modulates growth and ion partitioning in saltstressed wheat plants. J. Integr. Plant Biol 49: 1003-1015.

  • Khajeh-Hosseini M., Powell A.A.,Bingham I.J., 2003 - The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci. & Technol 31: 715-725.

  • Kaur S.A., Gupta K., Kaur N., 2002 - Effect of osmo and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism water deficit stress. Plant growth Regul 37: 17-22.

  • Kaya M.D., Gamze O., Atal M., Yakup C.,Ozer K., 2006 - Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur J Agron 24: 291-295.

  • McDonald M.B., 2000 - Seed priming. In: Seed Technology and Its Biological Basis (eds. M. Black and J.D. Bewley), Sheffield Acad. Press, Sheffield, England. pp. 287-326.

  • Murillo-Amador B., Lopez-Aguilar R.,Kaya C., Larrinaga-Mayoral J.,Flores-Hernandez A., 2002 - Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. J. Agronomy & Crop Science 188: 235-247.

  • Murungu F.S., Nyamugafata P., ChiduzaC., Clark L.J., Whalley W.R., 2003 - Effects of seed priming, aggregate size and soil matric potential on emergence of cotton (Gossypiumhirsutum L.) and maize (Zea mays L.). Soil and Tillage Research 74: 161-168.

  • Oram R.N., 1996 - Secale montanum - a wider role in Australia? NZ J. Agric. Res 39: 629-633.

  • Patade V.Y., Maya K., Zakwan A., 2011 - Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Res. J. Seed Sci 4 (3): 125 -136.

  • Redmann R.E., 1974 - Osmotic and specific ion effect on the germination of alfalfa. Canadian Journal of Botany 52: 803-808.

  • Robert A.B., Stephen B.M., R.H.

  • Abernethy, 1988 - Seeding competition between mountain rye. 'Hycrest' crested wheatgrass and downy brome. J. Range Manag. 41: 30-34.

  • Rouhi H.R., Aboutalebian M.A., Sharif-Zadeh F., 2011 - Effects of hydro and osmopriming on drought stress tolerance during germination in four grass species. International Journal of Agriscience, Vol 1 (2): 107-114.

  • Sadeghi H., Khazaei F., Yari L.,Sheidaei S., 2011 - Effect of seed osmopriming on seed germination behavior and vigor of soyebean (Glycine max L.). ARPN Journal of Agricultural and Biological Science, 6 (1): 39-43.

  • Sadeghian S.Y., Yavari N., 2004 - Effect of water-deficit stress on germination and early seedling growth in sugar beet. J. Agron. Crop Sci 190:138-144.

  • Salinas A.R., 1996 - Influence of Glycinemax (L.) Merrill seed quality on crop establishing and overcoming of ambiental stress. Pesquisa Agropecuaria Brasileira 31: 379-386.

  • Singh B., Usha K., 2003 - Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul 39: 137-141.

  • Tobeh A., Jamaati-e-Somarin Sh., 2012- Low temperature stress effect on wheat cultivars germination. African Journal of Microbiology Research 6(6): 1265-1269.

  • Tzortzakis N.G., 2009 - Effect of presowing treatment on seed germination and seedling vigour in endive and chicory. Hort. Sci (Prague) 36 (3): 117-125.

  • Zheng G.H., Wilen R.W., Slinkard A.E.,Gusta L.V., 1994 - Enhancement of canola seed germination and seedling emergence at low temperature by priming. Crop Science 34: 1589 -1593.


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