Principal Component Analysis of Chlorophyll Content in Tobacco, Bean and Petunia Plants Exposed to Different Tropospheric Ozone Concentrations

Open access


Three plant species were assessed in this study - ozone-sensitive and -resistant tobacco, ozone-sensitive petunia and bean. Plants were exposed to ambient air conditions for several weeks in two sites differing in tropospheric ozone concentrations in the growing season of 2009. Every week chlorophyll contents were analysed. Cumulative ozone effects on the chlorophyll content in relation to other meteorological parameters were evaluated using principal component analysis, while the relation between certain days of measurements of the plants were analysed using multivariate analysis of variance. Results revealed variability between plant species response. However, some similarities were noted. Positive relations of all chlorophyll forms to cumulative ozone concentration (AOT 40) were found for all the plant species that were examined. The chlorophyll b/a ratio revealed an opposite position to ozone concentration only in the ozone-resistant tobacco cultivar. In all the plant species the highest average chlorophyll content was noted after the 7th day of the experiment. Afterwards, the plants usually revealed various responses. Ozone-sensitive tobacco revealed decrease of chlorophyll content, and after few weeks of decline again an increase was observed. Probably, due to the accommodation for the stress factor. While during first three weeks relatively high levels of chlorophyll contents were noted in ozone-resistant tobacco. Petunia revealed a slow decrease of chlorophyll content and the lowest values at the end of the experiment. A comparison between the plant species revealed the highest level of chlorophyll contents in ozone-resistant tobacco.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Barret L.A. Bunce N.J. Gillespie T.J.: Estimation of tropospheric ozone production using concentrations of hydrocarbons and NOx and a comprehensive hydrocarbons reactivity parameter. Journal of Photochemistry and Photobiology A 113 (1998) 1-8.

  • 2. Borowiak K. Drzewiecka K. Zbierska J. Goliński P. Malicka M. Andrzejewska B.: Effect of tropospheric ozone on two white clover (Trifolium repens L. cv. ‘Regal’) clones with different ozone sensitivity exposed at rural area of Wielkopolska Region Archives of Environmental Protection 37 (2011a) 13-24.

  • 3. Borowiak K. Jusik S. Zbierska J.: Canonical Correspondence Analysis (CCA) as a tool for the interpretation of bioindication plants response to ambient air pollution Fresenius Environmental Bulletin 20(9) (2011b) 2264-2270.

  • 4. Della Torre G. Ferranti F. Lupattelli M. Pocceschi N. Figoli A. Nali C. Lorenzini G.: Effects of ozone on morpho-anatomy and physiology of Hedera helix Chemosphere 36 (1998) 651-656.

  • 5. Fowler D.: Ground-level ozone in the 21st century: future trends impacts and policy implications. London the Royal Society 2008.

  • 6. Greszta J. Gruszka A. Kowalkowska M.: Wpływ imisji na ekosystem. Katowice Wydawnictwo Naukowe Śląsk 2002.

  • 7. Hiscox J.D. Israelstam G.F.: A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57 (1978) 1332-1334.

  • 8. Laurence J.A. Amundson R.G. Friend A.L. Pell E.J. Temple P.J.: Allocation of carbon in plants under stress: An analysis of the ROPIS experiments. Journal of Environmental Quality 23 (1994) 412-417.

  • 9. Lejeune M Caliński T.: Canonical analysis applied to multivariate analysis of variance. Journal of Multivariate Analysis 72 (2000) 100-119.

  • 10. Long S.P. Naidu S.L.: Effects of oxidants at the biochemical cell and physiological level with particular reference to ozone in: Air pollution and plant life eds. J.N.B. Bell M. Treshow Chichester John Wiley and Sons 2004 69-89.

  • 11. Morgan P.B. Ainsworth E.A. Long S.P.: How does elevated ozone impact soybean? A meta-analysis of photosynthesis growth and yield. Plan Cell and Environment 26 (2003) 1317-1328.

  • 12. Raskin I.: Role of salicylic acid in plants. Annual Review of Plant Physiology and Plant Molecular Biology 43 (1992) 439-463.

  • 13. Saitanis C.J. Riga-Karandinos A.N. Karandinos M.G.: Effects of ozone on chlorophyll and quantum yield of tobacco (Nicotiana tabacum l.) varieties. Chemosphere 42 (2001) 945-953.

  • 14. Scebba F. Soldatini G. Ranieri A.: Ozone differentially affects physiological and biochemical response of two clover species; Trifolium repens and Trifolium pratense. Environmental Pollution 123 (2003) 209-216.

  • 15. Welfare K. Flowers T.J. Taylor G. Yeo A.R.: Additive and antagonistic effects of ozone and salinity on the growth ion content and gas exchange of five varieties of rice Oryza sativa L. Environmental Pollution 92 (1996) 257-266.

  • 16. Zbierska J. Borowiak K.: Wybrane reakcje zewnętrzne i wewnętrzne tytoniu szlachetnego na obecność ozonu troposferycznego. Zeszyty Naukowe AR Kraków - seria Inżynieria Środowiska 25 (2004) 377-388.

Journal information
Cited By
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 198 82 2
PDF Downloads 128 72 10