Anthelmintic residues in goat and sheep dairy products

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A multiresidue method (LC-MS/MS) for determination of wide range of anthelmintics was developed. The method covered benzimidazoles: albendazole (and metabolites), cambendazole, fenbendazol (and metabolites), flubendazole (and metabolites), mebendazole (and metabolites), oxibendazole, thiabendazole (and metabolites), triclabendazole (and metabolites); macrocyclic lactones: abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin; salicylanilides: closantel, ioxynil, nitroxynil, oxyclosamide, niclosamide, rafoxanid and others: clorsulon, derquantel, imidocarb, monepantel (and metabolites), morantel, praziquantel, and pyrantel. The method was used to examine the potential presence of anthelmintics in goat and sheep milk and dairy products from the Polish market. A total of 120 samples of milk, yoghurt, cottage cheese, cream cheese, and curd were analysed. None of the samples were found positive above CCα (1-10 μg/kg) except for one cottage cheese in which traces of albendazole sulfone were detected (5.2 ug/kg) and confirmed. The results of the study showed negligible anthelmintic residues in the goat and sheep milk and dairy products and confirm their good quality.

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  • 1. Aguilera-Luiz M.M. Vidal J.L.M. Romero-González R. Frenich A.G.: Multi-residue determination of veterinary drugs in milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry. J Chromatogr A 2008 1205 10-16.

  • 2. Anastasio A. Veneziano V. Capurro E. Rinaldi L. Cortesi M.L. Rubino R. Danaher M. Cringoli G.: Fate of eprinomectin in goat milk and cheeses with different ripening times following pour-on administration. J Food Prot 2005 68 1097-1101.

  • 3. Chen D. Tao Y. Liu Z. Liu Z. Huang L. Wang Y. Yuan Z.: Development of a high-performance liquid chromatography method to monitor the residues of benzimidazoles in bovine milk. J Chromatogr B Analyt Technol Biomed Life Sci 2010 878 2928-2932.

  • 4. Commission Decision 2002/657/EC of August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off J Eur Commun 2002 L 221 8-36.

  • 5. European Food Safety Authority (2012) Report for 2010 on the results from the monitoring of veterinary medicinal product residues and other substances in live animals and animal products.

  • 6. European Food Safety Authority Scientific Opinion: Scientific Opinion on the suitability of goat milk protein as a source of protein in infant formulae and in follow-on formulae. EFSA J 2012 10 2603-2610.

  • 7. Fleming S.A. Craig T. Kaplan R.M. Miller J.E. Navarre Ch. Rongs M.: Anthelmintic resistance of gastrointestinal parasites in small ruminants. J Vet Intern Med 2006 20 4350-4444.

  • 8. Fletouris D.J. Botsoglou N.A. Psomas I.E. Mantis A.I.: Albendazole-related drug residues in milk and their fate during cheesemaking ripening and storage. J Food Prot 1998 61 1484-1488.

  • 9. Furlani R.P.Z. Dias F.F.G. Nogueira P.M. Gomes F.M.L. Tfouni S.A.V. Camargo M.C.R.: Occurrence of macrocyclic lactones in milk and yogurt from Brazilian market. Food Control 2014 48 43-47.

  • 10. Gómez Pérez M.L. Romero-González R. Martínez Vidal J.L. Garrido Frenich A.: Analysis of veterinary drug residues in cheese by ultra-high-performance LC coupled to triple quadrupole MS/MS. J Sep Sci 2013 36 1223-1230.

  • 11. Imperiale F.A. Busetti M.R. Suárez V.H. Lanusse C.E.: Milk excretion of ivermectin and moxidectin in dairy sheep: assessment of drug residues during cheese elaboration and ripening period. J Agric Food Chem 2004 52 6205-6211.

  • 12. Imperiale F.A. Mottier L. Sallovitz J.M. Lifschitz A.L. Lanusse C.E.: Disposition of doramectin milk residues in lactating dairy sheep. J Agric Food Chem 2003 51 3185-3190.

  • 13. Imperiale F. Pis A. Sallovitz J. Lisfchitz A. Busetti M. Suárez V. Lanusse C.: Pattern of eprinomectin milk excretion in dairy sheep unaffected by lactation stage: comparative residual profiles in dairy products. J Food Prot 2006 69 2424-2429.

  • 14. Jedziniak P. Olejnik M. Rola J. Szprengier Juszkiewicz T.: Determination of anthemintic residues in dairy products. Food Addit Contamin 2015 (in press).

  • 15. Kinsella B. Byrne P. Cantwell H. Mccormack M. Furey A. Danaher M.: Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLCMS/ MS and QuEChERS method. J Chromatogr B Analyt Technol Biomed Life Sci 2011 879 3707-3713.

  • 16. Tsiboukis D. Sazakli E. Jelastopulu E. Leotsinidis M.: Anthelmintics residues in raw milk. Assessing intake by a children population. Pol J Vet Sci 2013 16 85-91.

  • 17. Whelan M. Chirollo C. Furey A. Cortesi M.L Anastasio A Danaher M.: Investigation of the persistence of levamisole and oxyclozanide in milk and fate in cheese. J Agric Food Chem 2010 58 12204-12209.

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