Antipruritic application of ovocystatin in atopic dermatitis in dogs - preliminary study

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Abstract

The study was an attempt to determine the possibilities of using ovocystatin, a component of a new generation product of natural origin, in local therapy of atopic dermatitis in dogs by suppressing pruritus during illness. Chicken egg cystatin was used locally in the interdigital spaces of forelimbs of dogs used in the experiment. The degree of pruritus and clinical changes in the animals were defined using CADESI-03 scale before and after the beginning of the experiment. The results obtained proved that ovocystatin may be used as a substance suppressing pruritus in atopic dermatitis.

1. Anastasi A., Brown M.A., Kembhavi A.A., Nicklin M.J., Sayers C.A., Sunter D.C., Barrett A.J.: Cystatin, a protein inhibitor of cystein proteinase. Improved purification from egg white, characterization and detection in chicken serum. Biochem J 1983, 211, 129-138.

2. Barrett A.J.: Cystatin, the egg white inhibitor of cysteine proteinase. Meth Enzymol 1981, 80, 771-778.

3. Bensignor E., Olivry T.: Treatment of localized lesions of canine atopic dermatitis with tacrolimus ointment: a blinded randomized controlled trial. Vet Dermatol 2005, 16, 52-60.

4. DeBoer D.J., Hiller A.: The ACVD task force on canine atopic dermatitis (XV): fundamental concepts in clinical diagnosis. Vet Immunol Immunopathol 2001, 81, 271-276.

5. DeRie M.A., Mainardi M.H.M., Dos J.: Lack of efficacy of topical cyclosporine A in atopic and allergic contact dermatitis. Acta Dermatol 1991, 71, 452-454.

6. Favrot C., Steffan J., Seewald W.: A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol 2010, 21, 23-30.

7. Gołąb K., Warwas M.: Chicken egg proteins- biochemical properties and applications. Adv Clin Exp Med 2005, 14, 1001-1010.

8. Gołąb K., Gburek J., Juszczyńska K., Trziszka T., Polanowski A.: Stabilization of monomeric hen’s egg white cyststin. Chem Industry 2012, 91, 741-744.

9. Gross T.L., Walder E.J., Ihrke P.J.: Subepidermal bullous dermatosis due to topical corticosteroid therapy in dogs. Vet Dermatol 1997, 8, 127-131.

10. Hill P.B., Lau P., Rybnicek J.: Development of an owner-assessed scale to measure the severity of pruritus in dogs. Vet Dermatol 2007, 18, 301-308.

11. Hill. P., Rybnicek J., Lau-Gillard P.: Correlation between pruritus score and grossly visible erythema in dogs. Vet Dermatol 2010, 21, 450-455.

12. Ikoma A., Steinhoff M., Stander S., Yosipovitch G., Schmelz M.: The neurobiology of itch. Nature Rev Neurosci 2006, 7, 534-547.

13. Machleidt W., Nagler D.K., Machleidt I.A., Stubbs M.T., Fritz H., Auerswald E.A.: Temporary inhibition of papain by hairpin loop mutants of Chicken Cystatin. Distorted binding of the loops results in cleavage of the Gly-Ala bond. FEBS Lett 1995, 361, 185-190.

14. Marsella R., Nicklin C.F., Sagilo S.: Investigation on the clnical efficacy and safety of 0.1% tacrolimus ointment (protopic) in canine atopic dermatitis: a randomized, double-blinded, placebo- controlled, cross-over study. Vet Dermatol 2004, 15, 294-303.

15. Migozuchi M., Kawaguchi K., Ohsauga Y., Ikary Y., Yanagawa A., Mizushima Y.: Cyclosporine ointment for psoriasis and atopic dermatitis. Lancet 1992, 339, 1120.

16. Nutall T., Mueller R., Bensignor E.: Efficacy of a 0.0584% hydrocortisone aceponante spray in management of canine atopic dermatitis: a randomised, double blind, placebo controlled trials. Vet Dermatol 2009, 20, 191-198.

17. Olivry T. Marsella R., Iwasaki T., Mueller R.: Validation of CADESI-03, a severity scale for clinical trials enrolling dogs with atopic dermatitis. Vet Dermatol 2007, 18, 78-86.

18. Olivry T., Mueller R., Nuttall T., Favrot C., Prelaud P.: Determination of CADESI-03 thresholds for increasing severity levels of canine atopic dermatitis. Vet Dermatol 2008, 19, 115-119.

19. Olivry T.: New diagnostic criteria for canine atopic dermatitis (Letter to the Editor). Vet Dermatol 2010, 21, 124-127

20. Olivry T., DeBoer D.J., Favrot C., Jackson H.A., Mueller R.S., Nuttall T., Prelaud P.: Treatment of canine atopic dermatitis: 2010 clinical practice guidelines from the International Task Force on Canine Atopic Dermatitis. Vet Dermatol 2010, 21, 233-248.

21. Rybnicek J., Lau-Gillard P.J., Harvey R., Hill P.B.: Further validation of a pruritus severity scale for use in dogs. Vet Dermatol 2009, 20, 115-122.

22. Saevik B.K., Bergvall K., Holm B.R., Saijonmaa- Koulumiess L.E., Hedhammar A., Larsen S., Kristensen F.: A randomized, controlled study to evaluate the steroid sparing effect of essential fatty acids supplementation in treatment of canine atopic dermatitis. Vet Dermatol 2004, 15, 137-145.

23. Trziszka T., Polanowski A., Saleh Y., Kopeć W.: Egg white isolation and its medical application. 2006, http://www.animalscience.com/uploads/additionalfiles/wpsaverona.htmL.

24. Umemoto T., Naito Y., Li M., Sazuki I., Namikawa I.: Growth inhibition of a human oral bacterium Porphyromonas gingivialis by rat cysteine proteinase inhibitor Cystatin S. Lett Appl Microbiol 1996, 23, 151-153.

25. Zerownik E., Cimerman N., Kos J., Turk V., Lohner K.: Thermal denaturatiom of human cystatin C and two of its variants.

Comparsion to chicken cystatin. Biol Chem 1997, 378, 1199-1203.

Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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