Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

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The aim of the study was to assess the distribution of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia canis in adult females and males of Ixodes ricinus and Dermacentor reticulatus ticks, inhabiting meadows near large forest complexes throughout the Lubelskie Voivodship (eastern region of Poland). Ticks were collected using the flagging method. Among 720 ticks collected, 506 were identified as D. reticulatus, and 214 as I. ricinus. DNA of B. canis and B. burgdorferi s.l. was detected in 21.3% and 0.6% of D. reticulatus ticks, respectively. In I. ricinus ticks, DNA specific to B. burgdorferi s.l. and A. phagocytophilum was detected in 5.6% and 10.3%, respectively. Co-infections of B. burgdorferi s.l. and A. phagocytophilum were found in two I. ricinus ticks. These results indicate that the Lublin region is an area at risk of tick-borne diseases of humans and animals, which must be considered in clinical practice.

1. Adaszek Ł., Winiarczyk S., Łukaszewska J.: A first case of ehrlichiosis in a horse in Poland. Dtsch Tierärztl Wochenschr 2009, 116, 330-334.

2. Adaszek Ł., Winiarczyk S.: Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland. Vet Parasitol 2008, 152, 235-241.

3. Bartosik K., Wiśniowski Ł., Buczek A.: Questing behavior of Dermacentor reticulatus adults (Acari: Amblyommidae) during diurnal activity periods in eastern Poland. J Med Entomol 2012, 49, 859-864.

4. Buczek A., Bartosik K.: Ticks (Ixodida: Ixodidae, Amblyommidae) in south-eastern Poland and their medical and epidemiological importance. Zdrow Publiczne 2011, 121, 392-397.

5. Cisak E., Wójcik-Fatla A., Stojek N., Chmielewska-Badora J., Zwoliński J., Buczek A., Dutkiewicz J.: Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from the Lublin region (eastern Poland). Ann Agric Environ Med 2006, 13, 301-306.

6. Földvári G., Márialigeti M., Solymosi N., Lukács Z., Majoros G., Kósa J.P., Farkas R.: Hard ticks infesting dogs in Hungary and their infection with Babesia and Borrelia species. Parasitol Res 2007, 101, 25-34.

7. Grzeszczuk A., Stańczak J.: Highly variable year-to-year prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks in North-Eastern Poland: A 4-year follow-up. Ann N Y Acad Sci 2006, 1078, 309-311.

8. Mizak B., Król J., Mizak Z.: Development of PCR and Nested- PCR for detection of Borrelia burgdorferi in dogs. Bull Vet Inst Pulawy 2000, 44, 11-20.

9. Siński E., Karbowiak G., Siuda K., Buczek A., Jongejan F.: Borrelia burgdorferi infection of ticks in some regions of Poland. Przegl Epidemiol 1994, 48, 461-465.

10. Siuda K.: Ticks (Acari: Ixodida) of Poland. Part II. Taxonomy and distribution. Polskie Towarzystwo Parazytologiczne, Warsaw 1993.

11. Skotarczak B., Wodecka B., Rymaszewska A., Sawczuk M., Maciejewska A., Adamska M., Hermanowska-Szpakowicz T., Świerbińska R.: Prevalence of DNA and antibodies to Borrelia burgdorferi sensu lato in dogs suspected of borreliosis. Ann Agric Environ Med 2005, 12, 199-205.

12. Sytykiewicz H., Karbowiak G., Werszko J., Czerniewicz P., Sprawka I., Mitrus J.: Molecular screening for Bartonella henselae and Borrelia burgdorferi sensu lato co-existence within Ixodes ricinus populations in central and eastern parts of Poland. Ann Agric Environ Med 2012, 19, 451-456.

13. Tokarz R., Jain K., Bennett A., Briese T., Lipkin W.I.: Assessment of polymicrobial infections in ticks in New York State. Vector Borne Zoonotic Dis 2010, 10, 217-221.

14. Wegner Z., Stańczak J., Racewicz M., Kruminis-Lozowska W., Kubica-Biernat B.: Occurrence of Borrelia spirochaetes in ticks (Acari, Ixodidae) collected in the forest areas in Olsztyn province (north central Poland). Bull Inst Marit Trop Med Gdynia 1993-1994, 44-45, 51-59.

15. Winiarczyk S., Adaszek Ł., Štefančíková A., Pet’tko B., Cislakova L., Puchalski A.: Serological investigations for borreliosis and ehrlichiosis in pig and cattle populations in the Lublin Voivodeship. Med Weter 2007, 63, 561-565.

16. Wójcik-Fatla A., Bartosik K., Buczek A., Dutkiewicz J.: Babesia microti in adult Dermacentor reticulatus ticks from eastern Poland. Vector Borne Zoonotic Dis 2012, 12, 841-843.

17. Wójcik-Fatla A., Szymańska J., Wdowiak L., Buczek A., Dutkiewicz J.: Coincidence of three pathogens (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti) in Ixodes ricinus ticks in the Lublin macroregion. Ann Agric Environ Med 2009, 16, 151-158.

18. Wójcik-Fatla A., Zając V., Cisak E., Sroka J., Sawczyn A., Dutkiewicz J.: Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland. Ann Agric Environ Med 2012, 19, 656-659.

19. Zygner W., Jaros S., Wedrychowicz H.: Prevalence of Babesia canis, Borrelia afzelii, and Anaplasma phagocytophilum infection in hard ticks removed from dogs in Warsaw (central Poland).Vet Parasitol 2008, 153, 139-142.

Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

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