Immune and oxidative parameters were evaluated as indicators of the influence of stress on the occurrence of respiratory syndrome in feedlot calves. The study was carried out on 60 2 and a half-month-old Simmentaler calves transported at feedlot. Mean daily feed consumption, daily weight gain, and behavioural activity were evaluated. Blood was collected on days 1, 3, 7, 14, 21, and 28 of feedlot. Serum NO ions, lipid peroxidation, acute phase proteins, IgG, and IgM were determined. The calves showed a decrease in feed consumption during the first 7 d of the feedlot. Sporadic stereotypies were noted. NO concentration showed a significant (P < 0.05) increase on days 7 and 14. Concentration of thiobarbituric acid reactive substances (TBARS) increased (P < 0.05) on days 1 and 3. IgM concentration was found to be considerably lower on days 14 and 28. Serum haptoglobin level showed a significant increase in stressed calves on days 1, 3, 7, and 14 of the feedlot. Based on these results, it can be suggested that stressors associated with transport and adaptation to the feedlot induce a stress reaction in calves, resulting in behavioural disorders, reduced weight gain, suppression of the humoral immunity and increased morbidity during the first weeks. These parameters seem to be crucial in evaluating the animals' health and welfare.
1. Bowland S.L., Shewen P.E.: Bovine respiratory disease: commercial vaccines currently available in Canada. Can Vet J 2000, 41, 33-48.
2. Chirase N.K., Greene L.W., Purdy C.W., Loan R.W., Auvermann B.W., Parker D.B., Walborg E.F., Stevenson D.E., Klaunig J.: Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle. Am J Vet Res 2004, 65, 860-864.
3. Council Directive 2008/119/EC of 18 December 2008 laying down minimum standards for the protection of calves. OJ L 10, 15.1.2009, p.7-13
4. Duff G.S., Galyean M.L.: Recent advances in management of highly stressed newly received feedlot cattle. J Anim Sci 2007, 85, 823-840.
5. Edwards A.J.: Respiratory disease of feedlot cattle in the central USA. Bovine Practitioner 1996, 30, 5-7.
6. Ellis J.A.: The immunology of the bovine respiratory disease complex. Vet Clinic North Am Food Anim Pract 2001, 17, 535-550.
7. Hodgson P.D., Aich P., Manuja A., Hokamp K., Roche F.M., Brinkman F.S.L., Potter A., Babiuk L.A., Griebel P.J.: Effect of stress on viral-bacterial synergy in bovine respiratory disease: novel mechanisms to regulate inflammation. Comp Funct Genom 2005, 6, 244-250.
8. Ishida N., Hobo S., Takahashi T., Nanbo Y., Sato F., Hasegawa T., Mukoyama H.: Chronological changes in superoxide-scavenging ability and lipid peroxide concentration of equine serum due to stress from exercise and transport. Equine Vet J Suppl 1999, 30, 430-433.
9. Ishiwata T., Uetake K., Eguchi Y., Tanaka T.: Seasonal and sex differences in area preference and behavior of young cattle just after long distance transport. Anim Sci J 2008, 79, 504-509.
10. Knowles T.G., Warris P.D., Brown S.N., Edwards J.E.: Effects on cattle of transportation by road for up to 31 hours. Vet Rec 1999, 145, 575-582.
11. Ledwożyw A., Michalak J., Stępień A., Kądziołka A.: The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human artheriosclerosis. Clin Chim Acta 1986, 155, 275-284.
12. Marin A., Pozza G., Gottardo F., Moro L., Stefani L., Cozzi G., Berscic M.: Administration of dexamethasone per os in finishing bulls. II. Effects on blood parameters used as indicators of animal welfare. Animal 2008, 7, 1080-1086.
13. Matsumoto K., Yobimoto K., Huong Thu Thi N., Fattah-Abdel M., Hien T.V., Watanable H.: Psychological stress - induced enhancement of brain lipid peroxidation via nitric oxide systems and its modulation by anxiolyc and anxiogenic drugs in mice. Brain Res 1999, 839, 74-84.
14. Orro T., Pohjanvirta T., Rikula U., Huovilainen A., Alasuutari S., Sihvonen L., Pelkonen S., Soveri T.: Acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus. Comp Immunol Microbiol Infect Dis 2011, 34, 23-29.
15. Rushen J., Marin A.: The motivation of non-nutritive sucking in calves, Bos taurus. Anim Behav 1995, 49, 1503-1510.
16. Snowder G.D., van Vleck L.D., Cundiff L.V., Bennett G.L., Koohmaraie M., Dikeman M.E.: Bovine respiratory disease in feedlot cattle: Phenotypic, environmental, and genetic correlations with growth, carcass, and longissimus muscle palatability traits. J Anim Sci 2007, 85, 1885-1892.
17. Uetake K., Ishiwata T., Tanaka T., Sato S.: Physiological responses of young cross-bred calves immediately after long- haul road transportation and after one week of habituation. Animal 2009, 80, 705-708.
18. Urban-Chmiel R., Hola P., Lisiecka U., Wernicki A., Puchalski A., Dec M., Wysocka M.: An evaluation of the effects of α- tocopherol and ascorbic acid in bovine respiratory disease complex occurring in feedlot calves after transport. Livest Sci 2011, 141, 53-58.
19. Urban-Chmiel R., Wernicki A., Puchalski A., Dec M.: The effect of α-tocopherol and ascorbic acid supplementation on selected immunological parameters in bovine leukocytes in vitro. Acta Vet Brno 2009, 78, 589-594.
20. Wernicki A., Urban-Chmiel R., Kankofer M., Mikucki P., Puchalski A., Tokarzewski S.: Evaluation of plasma cortisol and TBARS levels in calves after short-term transportation. Rev Med Vet 2006, 157, 30-34.
21. Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.: The influence of transport stress on the humoral immunological response of calves induced by M. haemolytica leukotoxin. Pol J Vet Sci 2003, 6, 40-45.
22. Zavy M.T., Juniewicz P.E., Phillips W.A., von Tungeln D.L.: Effects of initial restraint, weaning, and transport stress on baseline and ACTH stimulated cortisol responses in beef calves of different genotypes. Am J Vet Res 1992, 53, 551-560.