Isolation and identification of Mycoplasma mycoides cluster strains from goats in Chongqing, China

Open access


In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley’s medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

1. Adehan R.K., Ajuwape A.T.P., Adetosoye A.I., Alaka O.O.: Characterization of Mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Rumin Res 2006, 63, 44-49.

2. Alexander P.G., Slee K.J., McOrist S., Ireland L., Coloe P.J.: Mastitis in cows and polyarthritis and pneumonia caused by Mycoplasma species bovine group 7. Aust Vet J 1985, 62, 135-136.

3. Al-Aubaidi J.M., Dardiri A.H., Fabricant J.: Biochemical characterization and antigenic relationship of Mycoplasma mycoides subsp. mycoides, Freundt and Mycoplasma mycoides subsp. capri (Edward) Freundt. Int J Sys Evol Microbiol 1972, 22, 155-164.

4. Bascuñana C.R., Matisson J.G., Bölske G., Johansson K.E.: Characterization of the 16S rRNA genes from Mycoplasma sp. strain F38 and development of an identification system based on PCR. J Bacteriol 1994, 176, 2577-2586.

5. Bölske G., Mattsson J.G., Bascuňana C.R., Bergström K., Wesonga H., Johanson K.E.: Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp. capripneumoniae by PCR and REA. J Clin Microbiol 1996, 34, 785-791.

6. Connole M.D., Laws L., Hart R.K.: Mastitis in cattle caused by a Mycoplasma sp. Aust Vet J 1967, 43, 157-162.

7. Costas M., Leach R.H., Mitchelmore D.L.: Numerical analysis of PAGE protein patterns and the taxonomic relationships within the “Mycoplasma mycoides cluster”. J Gen Microbiol 1987, 133, 3319-3329.

8. Damassa A.J., Wakenell P.S., Brooks D.L.: Mycoplasma of goats and sheep. J Vet Diagn Invest 1992, 4, 101-113.

9. Fischer A., Shapiro B., Muriuki C., Heller M., Schnee C., Bongcam-Rudloff E., Vilei E.M., Frey J., Jores J.: The origin of the ‘Mycoplasma mycoides cluster’ coincides with domestication of ruminants. PLoS ONE 2012, 7, e36150.

10. Gil M.C., Hermoso de Mendoza M., Rey J., Alonso J.M., Poveda J.B., Hermoso de Mendoza J.: Aetiology of caprine contagious agalactia syndrome in Extremadura, Spain. Vet Rec 1999, 144, 24-25.

11. Hernandez L., Lopez J., St-Jacques M., Ontiveros L., Acosta J., Handl K.: Mycoplasma mycoides subsp. capri associated with goat respiratory disease and high flock mortality. Can Vet J 2006, 47, 366-369.

12. Hum S., Kessell A., Djordjevic S., Rheinberger R., Hornitzky M., Forbes W., Gonsalves J.: Mastitis, polyarthritis and abortion caused by Mycoplasma species bovine group 7 in dairy cattle. Aust Vet J 2000, 78, 744-750.

13. Kidanemariama A., Gouwsb J., van Vuurenb M., Gummow B.: In vitro antimicrobial susceptibility of Mycoplasma mycoides mycoides large colony and Arcanobacterium pyogenes isolated from clinical cases of ulcerative balanitis and vulvitis in Dorper sheep in South Africa. Tydskr S Afr Vet Ver 2005, 76, 204-208.

14. Kumar P., Roy A., Bhanderi B.B., Pal B.C.: Isolation, identification and molecular characterization of Mycoplasma isolates from goats of Gujarat State, India. Vet Arhiv 2011, 81, 443-458.

15. Kumar V., Rama R., Mehra S., Rout P.K.: Isolation and characterization of Mycoplasma mycoides subspecies capri from milk of natural goat mastitis cases. ISRN Vet Sci 2013, Article ID: 593029.

16. Leach R.H., Costas M., Mitchelmore D.L.: Relationship between Mycoplasma mycoides subsp. mycoides (“large-colony” strains) and M. mycoides subsp. capri, as indicated by numerical analysis of one-dimensional SDSPAGE protein patterns. J Gen Microbiol 1989, 135, 2993-3000.

17. Li Y., Xin J., Gao Y., Zhang J., Nicholas R.A., Lin Y.: Strains of Mycoplasma mycoides subsp. mycoides small colony type isolated in China between 1953 and 1960 show close similarity to strains of the Africa/Australia cluster. Vet J 2009, 179, 137-141.

18. Li Y., Zhang J.H., Hu S.P., Wang L., Xin J.Q.: Reclassification of the four China isolated strains of the pathogen for contagious caprine pleuropneumonia. Wei Sheng Wu Xue Bao 2007, 47, 769-773.

19. Manso-Silván L., Vilei E.M., Sachse K., Djordjevic S.P., Thiaucourt F., Frey J.: Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri. Int J Syst Evol Microbiol 2009, 59, 1353-1358.

20. Monnerat M.P., Thiaucourt F., Poveda J.B., Nicolet J., Frey J.: Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp. capri. Clin Diagn Lab Immunol 1999, 6, 224-230.

21. Olsson B., Bölske G., Bergstrom K., Johansson K.E.: Analysis of caprine mycoplasmas and mycoplasma infections in goats using two-dimensional electrophoresis and immunoblotting, Electrophoresis 1990, 11, 861-869.

22. Rodwell A.W.: The protein fingerprints of mycoplasmas. Rev Infect Dis 1982, 4, 8-17.

23. Shiel M.J., Coloe P.J., Worotniuk B., Burgess G.W.: Polyarthritis in a calf associated with group 7 mycoplasma infection. Aust Vet J 1982, 59, 192-193.

24. Simmons G.C., Johnston L.A.Y.: Arthritis in calves caused by Mycoplasma sp. Aust Vet J 1963, 39, 11-14.

25. Szeredi L., Tenk M., Dan A.: Infection of two goatherds with Mycoplasma mycoides subsp. capri in Hungary, evidence of a possible faecal excretion. J Vet Med B Infect Dis Vet Public Health 2003, 50, 172-177.

26. Thiaucourt F., Bölske G.: Contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats. Rev Sci Tech 1996, 15, 1397-1414.

27. Xin J., Li Y., Nicholas R.A., Chen C., Liu Y., Zhang M.J., Dong H.: A history of the prevalence and control of contagious bovine pleuropneumonia in China. Vet J 2012, 191, 166-170.

28. Vilei E.M., Korczak B.M., Frey J.: Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be grouped into a single subspecies. Vet Res 2006, 37, 779-790.

29. Villalba E.J., Poveda J.B., Fernández A., Rodriguez J.L., Gutierrez C., Gómez-Villamandos J.: An outbreak caused by Mycoplasma mycoides species in goats in the Canary Islands. Vet Rec 1992, 130, 330-331.

Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

Journal Information

IMPACT FACTOR 2017: 0.811
5-year IMPACT FACTOR: 0.973

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.287
Source Normalized Impact per Paper (SNIP) 2017: 0.484


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 97 97 16
PDF Downloads 28 28 6